Statistical classification of multivariate flow cytometry data analyzed by manual gating: Stem, progenitor, and epithelial marker expression in nonsmall cell lung cancer and normal lung

Cytometry Part A

Volumn 83 A, Issue 1, 2013, Pages 150-160

  Normolle, Daniel P ^a,b   Donnenberg, Vera S ^b,c,d   Donnenberg, Albert D ^b,c,d  

^a UNIVERSITY OF PITTSBURGH GRADUATE SCHOOL OF PUBLIC HEALTH   (United States)

^b UNIVERSITY OF PITTSBURGH CANCER INSTITUTE   (United States)

^c UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^d MCGOWAN INSTITUTE FOR REGENERATIVE MEDICINE   (United States)

Author keywords

Diagonal linear discriminant analysis; Elastic net; Lasso; Multivariate analysis; Nonsmall cell lung cancer; Normal lung; P > n problem; Random forest; Stem cells

Indexed keywords

BIOLOGICAL MARKER; CD133 ANTIGEN; GLYCOPROTEIN; HERMES ANTIGEN; KERATIN; LEUKOCYTE ANTIGEN; PEPTIDE; STEM CELL FACTOR RECEPTOR; TUMOR MARKER;

ADENOCARCINOMA; ARTICLE; COMPARATIVE STUDY; EPITHELIUM CELL; FLOW CYTOMETRY; HUMAN; LUNG; LUNG NON SMALL CELL CANCER; LUNG TUMOR; METABOLISM; METHODOLOGY; PATHOLOGY; SENSITIVITY AND SPECIFICITY; STEM CELL;

ADENOCARCINOMA; ANTIGENS, CD; ANTIGENS, CD44; BIOLOGICAL MARKERS; CARCINOMA, NON-SMALL-CELL LUNG; EPITHELIAL CELLS; FLOW CYTOMETRY; GLYCOPROTEINS; HUMANS; KERATINS; LUNG; LUNG NEOPLASMS; PEPTIDES; PROTO-ONCOGENE PROTEINS C-KIT; SENSITIVITY AND SPECIFICITY; STEM CELLS; TUMOR MARKERS, BIOLOGICAL;

EID: 84871656505     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22240     Document Type: Article

References (12)

1. Linderman MD, Bjornson Z, Simonds EF, Qiu P, Bruggner R, Sheode K, Meng TH, Plevritis SK, Nolan GP. CytoSPADE: High-Performance Analysis and Visualization of High-Dimensional Cytometry Data. Bioinformatics 2012; 28: 2400-2401.
2. Qiu P, Simonds EF, Bendall SC, Gibbs KDJr, Bruggner RV, Linderman MD, Sachs K, Nolan GP, Plevritis SK. Extracting a cellular hierarchy from high-dimensional cytometry data with SPADE. Nat Biotechnol 2011; 29: 886-891.
3. Bashashati A, Brinkman RR. A survey of flow cytometry data analysis methods. Adv Bioinfo 2009: 584603.
4. Hokanson JA, Rosenblatt JI, Leary JF. Some theoretical and practical considerations for multivariate statistical cell classification useful in autologous stem cell transplantation and tumor cell purging. Cytometry 1999; 36: 60-70.
5. Donnenberg VS, Landreneau RJ, Pfeifer ME, Donnenberg AD. Flow cytometric determination of stem/progenitor content in epithelial tissues: An example from non-small lung cancer and normal lung. Cytometry A 2013; 83A: 141-149.
6. Zimmerlin L, Donnenberg VS, Pfeifer ME, Meyer EM, Peault B, Rubin JP, Donnenberg AD. Stromal vascular progenitors in adult human adipose tissue. Cytometry A 2010; 77A: 22-30.
7. Donnenberg VS, Donnenberg AD, Zimmerlin L, Landreneau RJ, Bhargava R, Wetzel RA, Basse P, Brufsky AM. Localization of CD44 and CD90 positive cells to the invasive front of breast tumors. Cytometry B Clin Cytom 2010; 78B: 287-301.
8. R Development Core Team. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing; 2008.
9. Efron B. Bootstrap Methods: Another Look at the Jackknife. Ann Stat 1979; 7: 1-26.
10. Woo T, Okudela K, Mitsui H, Yazawa T, Ogawa N, Tajiri M, Yamamoto T, Rino Y, Kitamura H, Masuda M. Prognostic value of CD133 expression in stage I lung adenocarcinomas. Int J Clin Exp Pathol 2010; 4: 32-42.
11. Kajstura J, Rota M, Hall SR, Hosoda T, D'Amario D, Sanada F, Zheng H, Ogorek B, Rondon-Clavo C, Ferreira-Martins J, et al. Evidence for human lung stem cells. N Engl J Med 2011; 364: 1795-1806.
12. Al-Hajj M, Wicha MS, Benito-Hernandez A, Morrison SJ, Clarke MF. Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci USA 2003; 100: 3983-3988.