A novel flow cytometric assay for rapid detection of extended-spectrum beta-lactamases

Clinical Microbiology and Infection

Volumn 19, Issue 1, 2013, Pages

  Faria Ramos, I ^a   Espinar, M J ^a,b   Rocha, R ^a   Santos Antunes, J ^a,b   Rodrigues, A G ^a   Canton R ^c,d   Pina Vaz, C ^a,b  

^a University of Porto   (Portugal)

^b HOSPITAL DE SÃO JOÃO   (Portugal)

^c HOSPITAL RAMÓN Y CAJAL   (Spain)

^d CSIC   (Spain)

Author keywords

Drug susceptibility testing; Enterobacteriaceae; ESBLs; Flow cytometry

Indexed keywords

BETA LACTAMASE AMPC; BIS (1,3 DIBUTYLBARBITURIC ACID); CARBAPENEMASE; CEFOTAXIME; CEFTAZIDIME; CEPHALOSPORIN; CLAVULANIC ACID; EXTENDED SPECTRUM BETA LACTAMASE; FLUORESCENT DYE; OXACILLINASE; TRIMETHINE OXONOL; UNCLASSIFIED DRUG;

ACCURACY; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; BACTERIUM ISOLATE; CELL VIABILITY; COLONY FORMING UNIT; CONTROLLED STUDY; ENZYME ASSAY; ESCHERICHIA COLI; FLOW CYTOMETRY; FLUORESCENCE; KLEBSIELLA PNEUMONIAE; MEMBRANE DEPOLARIZATION; MEMBRANE PERMEABILITY; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAPID TEST;

BACTERIA (MICROORGANISMS); ENTEROBACTERIACEAE;

EID: 84871623767     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2012.03986.x     Document Type: Article

References (25)

1. Pitout JD. Infections with extended-spectrum β-lactamase-producing Enterobacteriaceae: changing epidemiology and drug treatment choices. Drugs 2010; 70: 313-333.
2. Schwaber MJ, Carmeli Y. Mortality and delay in effective therapy associated with extended-spectrum beta-lactamase production in Enterobacteriaceae bacteraemia: a systematic review and meta-analysis. J Antimicrob Chemother 2007; 60: 913-920.
3. Pfaller MA, Segreti J. Overview of the epidemiological profile and laboratory detection of extended-spectrum beta-lactamases. Clin Infect Dis 2006; 4: S153-S163.
4. Dallenne C, Da Costa A, Decré D et al. Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae. J Antimicrob Chemother 2010; 65: 490-495.
5. Stuart JC, Dierikx C, Naiemi NA et al. Rapid detection of TEM, SHV and CTX-M extended-spectrum b-lactamases in Enterobacteriaceae using ligation-mediated amplification with microarray analysis. J Antimicrob Chemother 2010; 65: 1377-1381.
6. Pitout JD, Hossain A, Hanson ND. Phenotypic and molecular detection of CTX-M-beta lactamases produced by Escherichia coli and Klebsiella spp. J Clin Microbiol 2004; 42: 5715-5721.
7. Shapiro HM. Multiparameter flow cytometry of bacteria: implications for diagnostics and therapeutics. Cytometry 2001; 43: 223-226.
8. Barbosa J, Costa-de-Oliveira S, Rodrigues AG et al. Optimization of a flow cytometry protocol for detection and viability assessment of Giardia lamblia. Travel Med Infect Dis 2008; 6: 234-239.
9. Pina-Vaz C, Sansonett F, Rodrigues AG et al. Susceptibility to fluconazole of Candida clinical isolates determined by FUN-1 staining with flow cytometry and epifluorescence microscopy. J Med Microbiol 2001; 50: 375-382.
10. Pina-Vaz C, Costa-de-Oliveira S, Rodrigues AG et al. Comparison of two probes for testing susceptibilities of pathogenic yeasts to voriconazole, itraconazole, and caspofungin by flow cytometry. J Clin Microbiol 2005; 43: 4674-4679.
11. Pina-Vaz C, Costa-de-Oliveira S, Rodrigues AG et al. Safe susceptibility testing of Mycobacterium tuberculosis by flow cytometry with the fluorescent nucleic acid stain SYTO, 16. J Med Microbiol 2005; 54: 77-81.
12. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 21th Informational Supplement. CLSI document M100-S21. Wayne, PA: Clinical and Laboratory Standards Institute, 2011.
13. Clinical and Laboratory Standards Institute. Performance standard for antimicrobial disk susceptibility tests. M2-A10. Wayne, PA: CLSI, 2009.
14. Espinar MJ, Rocha R, Ribeiro M et al. Extended-spectrum beta-lactamases (ESBLs) of Escherichia coli and Klebsiella pneumoniae screened by VITEK 2 System. J Med Microbiol 2011; 60: 756-760.
15. Miles AA, Misra SS. The estimation of the bactericidal power of the blood. J Hyg 1938; 38: 732-749.
16. Drawz SM, Bonomo RA. Three Decades of Beta-Lactamase Inhibitors. Clin Microbiol Rev 2010; 23: 160-201.
17. Cantón R, Morosini MI, de la Maza OM, de la Pedrosa EG. IRT and CMT beta-lactamases and inhibitor resistance. Clin Microbiol Infect 2008; 14 (suppl 1): 53-62.
18. Philippon A, Arlet G, Jacoby GA. Plasmid-determined AmpC-type beta-lactamases. Antimicrob Agents Chemother 2002; 46: 1-11.
19. Walberg M, Gaustad P, Steen HB. Rapid assessment of ceftazidime, ciprofloxacin, and gentamicin susceptibility in exponentially-growing E. coli by means of flow cytometry. Cytometry 1997; 27: 169-178.
20. Jepras RI, Paul FE, Pearson SC et al. Rapid assessment of antibiotic effects on Escherichia coli by bis-(1, 3-Dibutylbarbituric acid) trimethine oxonnol and flow cytometry. Antimicrob Agents Chemother 1997; 41: 2001-2005.
21. Jernaes MW, Steen HB. Staining of Escherichia coli for flow cytometry: influx and efflux of ethidium bromide. Cytometry 1994; 17: 302-309.
22. Gauthier C, St-Pierre Y, Villemur R. Rapid antimicrobial susceptibility testing of urinary tract isolates and samples by flow cytometry. J Med Microbiol 2002; 51: 192-200.
23. Mortimer FC, Mason DJ, Gant VA. Flow Cytometric monitoring of antibiotic-induced injury in Escherichia coli using cell-impermeant fluorescent probes. Antimicrob Agents Chemother 2000; 44: 676-681.
24. Roth BL, Poot M, Yue ST et al. Bacterial viability and antibiotic susceptibility testing with SYTOX green nucleic acid stain. Appl Environ Microbiol 1997; 63: 2421-2431.
25. Walberg M, Gaustad P, Steen HB. Rapid flow cytometric assessment of meticillin and ampicillin bacterial susceptibility. J Antimicrob Chemother 1996; 37: 1063-1075.