Optimized staining and proliferation modeling methods for cell division monitoring using cell tracking dyes

Journal of Visualized Experiments

Volumn , Issue 70, 2012, Pages

  Tario, Joseph D ^a   Humphrey, Kristen ^a   Bantly, Andrew D ^b   Muirhead, Katharine A ^c   Moore, Jonni S ^b   Wallace, Paul K ^a  

^a ROSWELL PARK CANCER INSTITUTE   (United States)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c Wiss Janney Elstner Associates Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE;

ARTICLE; CELL DIVISION; CELL LINE; CELL TRACKING; CHEMISTRY; CYTOLOGY; FLOW CYTOMETRY; HUMAN; INSTRUMENTATION; METHODOLOGY; MONOCYTE; MONONUCLEAR CELL; PHYSIOLOGY; STAINING;

CELL DIVISION; CELL LINE; CELL TRACKING; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; LEUKOCYTES, MONONUCLEAR; MONOCYTES; STAINING AND LABELING;

EID: 84871490688     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/4287     Document Type: Article

References (27)

1. Poon, R.Y., Ohlsson-Wilhelm, B.M., Bagwell, C.B., & Muirhead, K.A. Use of PKH Membrane Intercalating Dyes to Monitor Cell Trafficking and Function. In: Living Color: Flow Cytometry and Cell Sorting Protocols., Diamond, R.A. & DeMaggio, S., eds., Springer-Verlag, New York, NY, 302-352 (2000).
2. Wallace, P.K. & Muirhead, K.A. Cell Tracking 2007: A Proliferation of Probes and Applications. Immunol. Invest. 36, 527-562 (2007).
3. Hawkins, E.D., Hommel, M., Turner, M.L., Battye, F.L., Markham, J.F., & Hodgkin, P.D. Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data. Nat. Protoc. 2, 2057-2067 (2007).
4. Quah, B.J., Warren, H.S., & Parish, C.R. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester. Nat. Protoc. 2, 2049-2056 (2007).
5. Bolton, D.L., Minang, J.T., Trivett, M.T., Song, K., Tuscher, J.J., Li, Y., Piatak, Jr., M., O'Connor, D., Lifson, J.D., Roederer, M., & Ohlen, C. Trafficking, Persistence, and Activation State of Adoptively Transferred Allogeneic and Autologous Simian Immunodeficiency Virus-Specific CD8+ T Cell Clones during Acute and Chronic Infection of Rhesus Macaques. J. Immunol. 184, 303-314 (2010).
6. Juopperi, T.A. & Sharkis, S.J. Isolation of Quiescent Murine Hematopoietic Stem Cells by Homing Properties. Meth. Mol. Biol. 430, 21-30 (2008).
7. Kusumbe, A.P. & Bapat, S.A. Cancer stem cells and aneuploid populations within developing tumors are the major determinants of tumor dormancy. Cancer Res. 69, 9245-53 (2009).
8. Pece, S., Tosonim, D., Confalonieri, S., Mazzarol, G., Vecchi, M., Ronzoni, S., Bernard, L., Viale, G., Pelicci, P.G., & Di Fiore, P.P. Biological and Molecular Heterogeneity of Breast Cancers Correlates with Their Cancer Stem Cell Content. Cell. 140, 62-73 (2010).
9. Gertner-Dardenne, J., Poupot, M., Gray, B.D., & Fournié, J.-J. Lipophilic fluorochrome trackers of membrane transfers between immune cells. Immunol. Invest. 36, 665-685 (2007).
10. Bercovici, N., Givan, A.L., Waugh, M.G., Fisher, J.L., Vernel-Pauillac, F., Ernstoff, M.S., Abastado, J.P., & Wallace, P.K. Multiparameter precursor analysis of T-cell responses to antigen. J. Immunol. Methods. 276, 5-17 (2003).
11. Givan, A.L., Fisher, J.L., Waugh, M.G., Bercovici, N., & Wallace, P.K. Use of cell-tracking dyes to determine proliferation precursor frequencies of antigen-specific T cells. Methods Mol. Biol. 263, 109-24 (2004).
12. Schwaab, T., Tretter, C.P., Gibson, J.J., Cole, B.F., Schned, A.R., Harris, R., Fisher, J.L., Crosby, N., Stempkowski, L.M., Heaney, J.A., & Ernstoff, M.S. Tumor-related immunity in prostate cancer patients treated with human recombinant granulocyte monocyte-colony stimulating factor (GM-CSF). Prostate. 66 (6), 667-674 (2006).
13. Bantly, A.D., Gray, B.D., Breslin, E., Weinstein, E.G., Muirhead, K.A., Ohlsson-Wilhelm, B.M., & Moore, J.S. CellVue Claret, a New Far-Red Dye, Facilitates Polychromatic Assessment of Immune Cell Proliferation. Immunol. Invest. 36, 581-605 (2007).
14. Givan, A.L. A flow cytometric assay for quantitation of rare antigen-specific T-cells: using cell-tracking dyes to calculate precursor frequencies for proliferation. Immunol. Invest. 36, 563-580 (2007).
15. Tario, J.D., Jr., Gray, B.D., Wallace, S.S., Muirhead, K.A., Ohlsson-Wilhelm, B.M., & Wallace, P.K. Novel lipophilic tracking dyes for monitoring cell proliferation. Immunol Invest. 36, 861-885 (2007).
16. Wallace, P.K., Tario, Jr. J.D., Fisher, J.L., Wallace, S.S., Ernstoff, M.S., & Muirhead, K.A. Tracking Antigen-Driven Responses by Flow Cytometry: Monitoring Proliferation by Dye Dilution. Cytometry. 73A, 1019-1034 (2008).
17. Barth, R.J., Fisher, D.A., Wallace, P.K. Channon, J.Y., Noelle, R.L., Gui, J., & Ernstoff, M.S. A Randomized Trial of Ex vivo CD40L Activation of a Dendritic Cell Vaccine in Colorectal Cancer Patients: Tumor-Specific Immune Responses Are Associated with Improved Survival. Clin. Cancer Res. 16, 5548-5556 (2010).
18. Tario, J.D., Jr., Muirhead, K.A., Pan, D., Munson, M., & Wallace, P.K. Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry. Meth. Mol. Biol. 699, 119-164 (2011).
19. Fuse, S. & Underwood, E. Simultaneous Analysis of In Vivo CD8+ T Cell Cytotoxicity Against Multiple Epitopes using Multicolor Flow Cytometry. Immunol. Invest. 36, 829-845 (2007).
20. Schütz, C., Fleck, M., Mackensen, A., Zoso, A., Halbritter, D., Schneck, J.P., & Oelke, M. Killer artificial antigen-presenting cells: a novel strategy to delete specific T cells. Blood. 111, 3546-3552 (2008).
21. Zaritskaya, L., Shurin, M.R., Sayers, T.J., & Malyguine, A.M. New flow cytometric assays for monitoring cell-mediated cytotoxicity. Expert Rev. Vaccines. 9, 601-616 (2010).
22. Roederer, M. Interpretation of cellular proliferation data: Avoid the panglossian. Cytometry. 79A, 95-101 (2011).
23. Personal communication, E. Breslin (Zynaxis Cell Science, Inc.).
24. Quah, B.J.C. & Parish, C.R. The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation. J. Vis. Exp. (44), e2259, doi:10.3791/2259 (2010).
25. http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/mini26bul.Par.0001.File.tmp/mini26bul.pdf (Sigma product bulletin for PKH26).
26. Houlihan, D.D. & Newsome, P.N. Critical Review of Clinical Trials of Bone Marrow Stem Cells in Liver Disease. Gastroenterology. 135, 438-450 (2008).
27. Brusko, T.M., Hulme, M.A., Myhr, C.B., Haller, M.J., & Atkinson, M.A. Assessing the In Vitro Suppressive Capacity of Regulatory T Cells. Immunol. Invest. 36, 607-628 (2007).