Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

BMB Reports

Volumn 45, Issue 10, 2012, Pages 589-594

  Cha, Mijeong ^a   Shim, Sang Hee ^a   Kim, Sung Hong ^b   Kim, Ok Tae ^c   Lee, Se Weon ^d   Kwon, Suk Yoon ^e   Baek, Kwang Hyun ^a  

^a Yeungnam University   (South Korea)

^b Korea Basic Science Institute (KBSI)   (South Korea)

^c Rural Development Administration   (South Korea)

^d National Institute of Animal Science   (South Korea)

^e Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Panax ginseng C.A. Meyer; Root culture; Taxadiene synthase; Taxol; Transformation

Indexed keywords

ALKENE; DITERPENE; ISOMERASE; JASMINIC ACID; LINOLEIC ACID; PACLITAXEL; TAXA 4(5),11(12) DIENE SYNTHASE; TAXA 4(5),11(12)DIENE; TAXA-4(5),11(12)-DIENE SYNTHASE; TAXA-4(5),11(12)DIENE;

ARTICLE; BIOSYNTHESIS; CELL CULTURE; CHEMISTRY; CYTOLOGY; DRUG EFFECT; GENETICS; METABOLISM; PANAX; PLANT CELL; PLANT ROOT;

ALKENES; CELLS, CULTURED; DITERPENES; ISOMERASES; LINOLEIC ACIDS; PACLITAXEL; PANAX; PLANT CELLS; PLANT ROOTS;

PANAX GINSENG; TAXUS; TAXUS BREVIFOLIA;

EID: 84871446061     PISSN: 19766696     EISSN: 1976670X     Source Type: Journal    
DOI: 10.5483/BMBRep.2012.45.10.085     Document Type: Article

References (35)

1. Wani, M. C., Taylor, H. L., Wall, M. E., Coggan, P. and McPhail, A. T. (1971) Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J. Am. Chem. Soc. 93, 2325-2327.
2. Kumar, N. (1981) Taxol-induced polymerization of purified tubulin: mechanism of action. J. Biol. Chem. 256, 10435-10441.
3. Parness, J. and Horwitz, S. B. (1981) Taxol binds to polymerized tubulin in vitro. J. Cell Biol. 91, 479-487.
4. Ahn, J.-H., Eum, K.-H. and Lee, M. (2010) Spry2 does not directly modulate Raf-1 kinase activity in v-Ha-ras-transformed NIH 3T3 fibroblasts. BMB Rep. 43, 205-211.
5. Dieras, V. (1998) Taxanes in combination with doxorubicin in the treatment of the treatment of metastatic breast cancer. Semin. Oncol. 25, 18-22.
6. Sgadari, C., Toschi, E., Palladino, Clelia., Barillari, Giovanni., Carlei, D., Cereseto, A., Ciccolella, C., Yarchoan, R., Monini, P., Stürzl, M. and Ensoli, B. (2000) Mechanism of paclitaxel activity in Kaposi's sarcoma. J. Immunol. 165, 509-517.
7. Pezzutto, J. (1996) Taxol production in plant cell culture comes of age. Nat. Biotechnol. 14, 1083.
8. Rowinsky, E. K. and Donehower, R. C. (1995) Paclitaxel (Taxol). N. Engl. J. Med. 332, 1004-1014.
9. Woo, D. D. L., Miao, S. Y. P., Pelayo, J. C. and Woolf, A. S. (1994) Taxol inhibits progression of congenital polycystic kidney disease. Nature 368, 750-753.
10. Arsenaulta, A. L., Lhotáka, Š., Hunter, W. L., Banquerigoc, M. L. C. and Brahn, E. (1998) Taxol involution of collagen- induced arthritis: Ultrastructural correlation with the inhibition of synovitis and neovascularization. Clin. Immunol. Immunopathol. 86, 280-289.
11. Vidensek, N., Lim, P., Campbell, A. and Carlson, C. (1990) Taxol content in bark, wood, root, leaf, twig, and seedling from several Taxus species. J. Nat. Prod. 53, 1609-1610.
12. Holton, R. A., Kim, H. B., Somoza, C., Liang, F., Biediger, R. J., Boatman, P. D., Shindo, M., Smith, C. C., Kim, S., Nadizadeh, H., Suzuki, Y., Tao, C., Vu, P., Tang, S., Zhang, P., Murthi, K. K., Gentile, L. N. and Liu, J. H. (1994) First total synthesis of taxol. 2. Completion of the C and D rings. J. Am. Chem. Soc. 116, 1599-1600.
13. Holton, R. A., Somoza, C., Kim, H. B., Liang, F., Biediger, R. J., Boatman, P. D., Shindo, M., Smith, C. C., Kim, S., Nadizadeh, H., Suzuki, Y., Tao, C., Vu, P., Tang, S., Zhang, P., Murthi, K. K., Gentile, L. N. and Liu, J. H. (1994) First total synthesis of taxol. 1. Functionalization of the B ring. J. Am. Chem. Soc. 116, 1597-1598.
14. Nicolaou, K. C., Nantermet, P. G., Ueno, H., Guy, R. K., Couladouros, E. A. and Sorensen, E. J. (1995. Total synthesis of taxol. 1. Retrosynthesis, degradation, and reconstitution. J. Am. Chem. Soc. 117, 624-633.
15. Danishefsky, S. J., Masters, J. J., Young, W. B., Link, J. T., Snyder, L. B., Magee, T. V., Jung, D. K., Isaacs, R. C. A., Bornmann, W. G., Alaimo, C. A., Coburn, C. A. and Di Grandi, M. J. (1996) Total synthesis of baccatin III and taxol. J. Am. Chem. Soc. 118, 2843-2859.
16. Castor, T. P. and Theodore, A. T. (1993) Determination of taxol in Taxus media needles in the presence of interfering components. J. Liqu. Chromatogr. 16, 723-731.
17. Stierle, A., Strobel, G. and Stierle, D. (1993) Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of pacific yew. Science 260, 214-216.
18. Li, J. Y., Stroble, G. Sidhy, R., Hess, W. M. and Ford, E. J. (1996) Endophytic taxol-producing fungi from bald cypress, Taxodium distichum. Microbiol. 142, 2223-2226.
19. Kim, J. H., Yun, J. H., Hwang, Y. S., Byun, S. Y. and Kim, D. I. (1995) Production of taxol and related taxanes in Taxus brevifolia cell cultures: effect of sugar. Biotechnol. Lett. 17, 101-106.
20. Srinivasan, V., Pestchanker, L., Moser, S., Hirasuna, T. J., Taticek, R. A. and Schuler, M. L. (1995) Taxol production in bioreactors: kinetics of biomass accumulation, nutrient uptake, and taxol production by cell suspension of Taxus baccata. Biotechnol. Bioeng. 47, 666-676.
21. Cusidó, R. M., Palazón, J., Bonfill, M., Navia-Osorio, A., Morales, C. and Piñol, M. T. (2002) Improved paclitaxel and baccatin III production in suspension cultures of Taxus media. Biotechnol. Prog. 18, 418-423.
22. Zhang, L., Ding, R., Chai, Y., Bonfill, M., Moyano, E., Oksman-Caldentey, K. M., Xu, T., Pi, Y., Wang, Z., Zhang, H., Kai, G., Liao, Z., Sun, X. and Tang, K. (2004) Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures. Proc. Natl. Acad. Sci. U.S.A. 101, 6786-6791.
23. David, C., Chilton, M. D. and Tempé, J. (1984) Conservation of T-DNA in plants regenerated from hairy root cultures. Nature Biotech. 2, 73-76.
24. Kim, J. A., Baek, K. H., Son, Y. M., Son, S. H. and Shin, H. S. (2009) Hairy Root cultures of Taxus cuspidata for en hanced production of Paclitaxel. JKSABC. 52, 144-150.
25. Walker, K. and Croteau, R. (2001) Molecules of interest: Taxol biosynthetic genes. Phytochem. 58, 1-7.
26. Hezari, M., Lewis, N. G. and Croteau, R. (1995) Purification and characterization of taxa-4(5),11(12)-diene synthase from pacific yew (Taxus brevifolia) that catalyzes the first committed step of Taxol biosynthesis. Arch. Biochem. Biophys. 322, 437-444.
27. Wang, W., Shi, Q., Zhu, P., Ouyang, T., Li, N. and Cheng, K. D. (2002) cDNA cloning, expression and characterization of taxadiene synthase, a diterpene cyclase from Taxus chinensis. Acta. Bot. Sin. 44, 181-187.
28. Kim, D. Y., Jung, M. S., Park, Y. G, Yuan, H. D., Quan, H. Y. and Chung, S. H. (2011) Ginsenoside Rh2(S) induces the differentiation and mineralization of osteoblastic MC3T3-E1 cells through activation of PKD and p38 MAPK pathways. BMB Rep. 44, 659-664.
29. Kovacs, K., Zhang, L., Linforth, R. S. T., Whittaker, B., Hayes, C. J. and Fray, R. G. (2007) Redirection of carotenoid metabolism for the efficient production of taxadiene [taxa-4(5),11(12)-diene] in transgenic tomato fruit. Transgenic Res. 16, 121-126.
30. Anterola, A., Shanle, E., Perroud, P. and Quatrano, R. (2009) Production of taxa-4(5),11(12)-diene by transgenic Physcomitrella patens. Transgenic Res. 18, 655-660.
31. Besumbes, O., Sauret-Gueeto, S., Phillips, M. A., Imperial, S., Rodriguez-Concepcion, M., Boronat, A. (2004) Metabolic engineering of isoprenoid biosynthesis in Arabidopsis for the production of taxadiene, the first committed precursor of taxol. Biotechnol. Bioeng. 88, 168-175.
32. Yasumura, Y., Crumpton-Taylor, M., Fuentes, S., Harberd, N. P. (2007) Step-by-step acquisition of the gibberellin- DELLA growth-regulatory mechanism during land-plant evolution. Curr. Biol. 17, 1225-1230.
33. Hood, E. E., Gelvin, S. B., Melchers, L. S. and Hoekema, A. (1993) New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res. 2, 208-218.
34. Valvekens, D., Montagu, M. V. and Lijsebettens, M. V. (1988) Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc. Natl. Acad. Sci. U.S.A. 85, 5536-5540.
35. Dellaporta, S. L., Wood, J. and Hicks, J. B. (1983) A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep. 1, 19-21.