Allelic inhibition of displacement activity: A simplified one tube allele-specific PCR for evaluation of ITPA polymorphisms

Journal of Virological Methods

Volumn 187, Issue 2, 2013, Pages 271-273

  Galmozzi, E ^a   Facchetti, F ^a   Degasperi, E ^a   Aghemo, A ^a   Lampertico, P ^a  

^a UNIVERSITY OF MILAN   (Italy)

Author keywords

Allelic discrimination PCR; Hepatitis C virus; Inosine triphosphatase; Ribavirin

Indexed keywords

ACID ANHYDRIDE HYDROLASE; DNA; INOSINE TRIPHOSPHATASE; PEGINTERFERON; RIBAVIRIN; UNCLASSIFIED DRUG;

ACCURACY; ALLELE; ALLELIC INHIBITION OF DISPLACEMENT ACTIVITY ASSAY; ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; GENE; GENETIC ASSOCIATION; GENOTYPE; GOLD STANDARD; HEMOLYTIC ANEMIA; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; INTERMETHOD COMPARISON; ITPA GENE; MAJOR CLINICAL STUDY; NUCLEIC ACID ANALYSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SINGLE NUCLEOTIDE POLYMORPHISM;

ALLELES; ANEMIA, HEMOLYTIC; ANTIVIRAL AGENTS; HEPATITIS C, CHRONIC; HUMANS; INTERFERONS; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; PYROPHOSPHATASES; RIBAVIRIN; SENSITIVITY AND SPECIFICITY;

HEPATITIS C VIRUS;

EID: 84871421345     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.11.026     Document Type: Article

References (12)

1. Arenas M., Duley J., Sumi S., Sanderson J., Marinaki A. The ITPA c.94C>A and g.IVS2+21>C sequence variants contribute to missplicing of the ITPA gene. Biochim. Biophys. Acta 2007, 1772:96-102.
2. Atanasova S., Shipkova M., Svinarov D., Mladenova A., Genova M., Wieland E., Oellerich M., von Ahsen N. Analysis of ITPA phenotype-genotype correlation in the Bulgarian population revealed a novel gene variant in exon 6. Ther. Drug Monit. 2007, 29:6-10.
3. Fellay J., Thompson A.J., Ge D., Gumbs C.E., Urban T.J., Shianna K.V., Little L.D., Qiu P., Bertelsen A.H., Watson M., Warner A., Muir A.J., Brass C., Albrecht J., Sulkowski M., McHutchison J.G., Goldstein D.B. ITPA gene variants protect against anaemia in patients treated for chronic hepatitis C. Nature 2010, 464:405-408.
4. Galmozzi E., Del Menico B., Rametta R., Dongiovanni P., Fracanzani A.L., Benedan L., Borroni V., Maggioni P., Fargion S., Valenti L. A tetra-primer amplification refractory mutation system polymerase chain reaction for the evaluation of rs12979860 IL28B genotype. J. Viral Hepat. 2011, 18:628-630.
5. Hitomi Y., Cirulli E.T., Fellay J., McHutchison J.G., Thompson A.J., Gumbs C.E., Shianna K.V., Urban T.J., Goldstein D.B. Inosine triphosphate protects against ribavirin-induced adenosine triphosphate loss by adenylosuccinate synthase function. Gastroenterology 2011, 140:1314-1321.
6. Lavanchy D. The global burden of hepatitis. Liver Int. 2009, 29:74-81.
7. Lewis A.P., Sims M.J., Gewert D.R., Peakman T.C., Spence H., Crowe J.S. Taq DNA polymerase extension of internal primers blocks polymerase chain reactions allowing differential amplification of molecules with identical 5' and 3' ends. Nucleic Acids Res. 1994, 22:2859-2861.
8. Li W., Zeng W., Wang J., Zhou B., Zhang J., Zhang H., Li J., Wu Y., Hamoudi R., Zhou Y. Predicting sustained viral response to hepatitis C using a rapid and simple IL28B rs8099917 genotyping assay. Antiviral Res. 2012, 94:54-56.
9. McHutchison J.G., Lawitz E.J., Shiffman M.L., Muir A.J., Galler G.W., McCone J., Nyberg L.M., Lee W.M., Ghalib R.H., Schiff E.R., Galati J.S., Bacon B.R., Davis M.N., Mukhopadhyay P., Koury K., Noviello S., Pedicone L.D., Brass C.A., Albrecht J.K., Sulkowski M.S., IDEAL Study Team Peginterferon-2b or -2a with ribavirin for treatment of hepatitis C infection. N. Engl. J. Med. 2009, 361:580-593.
10. Stepchenkova E.I., Tarakhovskaya E.R., Spitler K., Frahm C., Menezes M.R., Simone P.D., Kolar C., Marky L.A., Borgstahl G.E., Pavlov Y.I. Functional study of the P32T ITPA variant associated with drug sensitivity in humans. J. Mol. Biol. 2009, 392:602-613.
11. Thompson A.J., Fellay J., Patel K., Tillmann H.L., Naggie S., Ge D., Urban T.J., Shianna K.V., Muir A.J., Fried M.W., Afdhal N.H., Goldstein D.B., McHutchison J.G. Variants in the ITPA gene protect against ribavirin-induced hemolytic anemia and decrease the need for ribavirin dose reduction. Gastroenterology 2010, 139:1181-1189.
12. Ye S., Dhillon S., Ke X., Collins A.R., Day I.N. An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res. 2001, 29:1-8.