CDNA cloning, expression, and enzymatic activity of a novel endogenous cellulase from the beetle Batocera horsfieldi

Gene

Volumn 514, Issue 1, 2013, Pages 62-68

  Xia, Dingguo ^a,b   Wei, Yadong ^b   Zhang, Guozheng ^b   Zhao, Qiaoling ^b   Zhang, Yeshun ^b   Xiang, Zhonghuai ^a   Lu, Cheng ^a  

^a SOUTHWEST UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Batocera horsfieldi; Beetle; CDNA cloning; Cellulase; Enzymatic activity; Insect

Indexed keywords

AMINO ACID; BETA 1,4 ENDOGLUCANASE; CELLULASE; COMPLEMENTARY DNA; GLYCOSIDASE; UNCLASSIFIED DRUG;

ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; BATOCERA HORSFIELDI; BEETLE; BOMBYX MORI; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; EXON; GENE EXPRESSION; GENETIC CODE; HEMOLYMPH; INSECT CELL; LARVA; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PIR-INTERNATIONAL PROTEIN SEQUENCE DATABASE; PRIORITY JOURNAL; PROTEIN GLYCOSYLATION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BEETLES; BOMBYX; CATALYTIC DOMAIN; CELLULASE; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA, COMPLEMENTARY; GENE EXPRESSION; GENES, INSECT; INSECT PROTEINS; MOLECULAR SEQUENCE DATA; PHYLOGEOGRAPHY; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

APRIONA GERMARI; BATOCERA HORSFIELDI; BOMBYX MORI; COLEOPTERA; HEXAPODA;

EID: 84871407705     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2012.08.044     Document Type: Article

References (32)

1. Attwood G.T., Herrera F., Weissenstein L.A., White B.A. An endo-beta-1,4-glucanase gene (celA) from the rumen anaerobe Ruminococcus albus: cloning, sequencing, and transcriptional analysis. Can. J. Microbiol. 1996, 42:267-278.
2. Béguin P. Detection of cellulase activity in polyacrylamide gels using congo red stained agar replicas. Anal. Biochem. 1983, 131:333-336.
3. Béguin P., Aubert J.P. The biological degradation of cellulose. FEMS Microbiol. Rev. 1994, 13:25-58.
4. Bourne Y., Henrissat B. Glycoside hydrolases and glycosyltransferases: families and functional modules. Curr. Opin. Struct. Biol. 2001, 11:593-600.
5. Breznak J.A., Brune A. Role of microorganisms in the digestion of lignocellulose by termites. Annu. Rev. Entomol. 1994, 39:453-487.
6. Carpita N.C., Gibeaut D.M. Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J. 1993, 3:1-30.
7. Das K.M., Tripathi S.D. Studies on the digestive enzymes of grass carp, Ctenopharyngodon idella (Val.). Aquaculture 1991, 92:21-32.
8. del Campillo E., Bennett A.B. Pedicel breakstrength and cellulase gene expression during tomato flower abscission. Plant Physiol. 1996, 111:813-820.
9. Girard C., Jouanin L. Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae. Insect Biochem. Mol. Biol. 1999, 29:1129-1142.
10. Henrissat B., Driguez H., Viet C., Schulein M. Synergism of cellulases from Trichoderma reesei in the degradation of cellulose. Nat. Biotechnol. 1985, 3:722-726.
11. Lee S.J., et al. cDNA cloning, expression, and enzymatic activity of a cellulase from the mulberry longicorn beetle, Apriona germari. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2004, 139:107-116.
12. Lee S.J., et al. A novel cellulase gene from the mulberry longicorn beetle, Apriona germari: gene structure, expression, and enzymatic activity. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2005, 140:551-560.
13. Levin R.J. Digestion and absorption of carbohydrates-from molecules and membranes to humans. Am. J. Clin. Nutr. 1994, 59:690S-698S.
14. Li L., Frohlich J., Pfeiffer P., Konig H. Termite gut symbiotic archaezoa are becoming living metabolic fossils. Eukaryot. Cell 2003, 2:1091-1098.
15. Li W., Huan X., Zhou Y., Ma Q., Chen Y. Simultaneous cloning and expression of two cellulase genes from Bacillus subtilis newly isolated from Golden Takin (Budorcas taxicolor Bedfordi). Biochem. Biophys. Res. Commun. 2009, 383:397-400.
16. Miller G.L. Use of the dinitrosalicylic acid reagent for the determination of reducing sugars. Anal. Chem. 1959, 31:426-428.
17. Motohashi T., Shimojima T., Fukagawa T., Maenaka K., Park E.Y. Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system. Biochem. Biophys. Res. Commun. 2005, 326:564-569.
18. Nakashima K., Watanabe H., Saitoh H., Tokuda G., Azuma J.I. Dual cellulose-digesting system of the wood-feeding termite, Coptotermes formosanus Shiraki. Insect Biochem. Mol. Biol. 2002, 32:777-784.
19. Ohtoko K., Ohkuma M., Moriya S., Inoue T., Usami R., Kudo T. Diverse genes of cellulase homologues of glycosyl hydrolase family 45 from the symbiotic protists in the hindgut of the termite Reticulitermes speratus. Extremophiles 2000, 4:343-349.
20. O'Reilly D.R., Miller L.K., Luckow V.A. Baculovirus Expression Vectors: A Laboratory Manual 1992, W.H. Freeman and Co., New York.
21. Rehman F.U., et al. Isolation of cellulolytic activities from Tribolium castaneum (red flour beetle). Afr. J. Biotechnol. 2009, 23:6710-6715.
22. Scharf M.E., Wu-Scharf D., Pittendrigh B.R., Bennett G.W. Caste-and development-associated gene expression in a lower termite. Genome Biol. 2003, 4:R62.1-R62.11.
23. Scharf M.E., Wu-Scharf D., Zhou X., Pittendrigh B.R., Bennett G.W. Gene expression profiles among immature and adult reproductive castes of the termite Reticulitermes flavipes. Insect Mol. Biol. 2005, 14:31-44.
24. Sugimura M., Watanabe H., Lo N., Saito H. Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris. Eur. J. Biochem. 2003, 270:3455-3460.
25. Taherzadeh M.J., Karimi K. Pretreatment of lignocellulosic wastes to improve ethanol and biogas production: a review. Int. J. Mol. Sci. 2008, 9:1621-1651.
26. Tamura K., Dudley J., Nei M., Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007, 24:1596-1599.
27. Tokuda G., Lo N., Watanabe H., Slaytor M., Matsumoto T., Noda H. Metazoan cellulase genes from termites: intron/exon structures and sites of expression. Biochim. Biophys. Acta 1999, 1447:146-159.
28. Watanabe H., Noda H., Tokuda G., Lo N. A cellulase gene of termite origin. Nature 1998, 394:330-331.
29. Wei Y.D., et al. N-linked glycosylation of a beetle (Apriona germari) cellulase Ag-EGase II is necessary for enzymatic activity. Insect Biochem. Mol. Biol. 2006, 36:435-441.
30. Xiang J., et al. Expression and characterization of recombinant VP19c protein and N-terminal from duck enteritis virus. Virol. J. 2011, 8:82.
31. Xue X.M., Anderson A.J., Richardson N.A., Anderson A.J., Xue G.P., Mather P.B. Characterisation of cellulase activity in the digestive system of the redclaw crayfish (Cherax quadricarinatus). Aquaculture 1999, 180:373-386.
32. Yoon H.J., Mah Y.I. Life cycle of the mulberry longicorn beetle, Apriona germari Hope on an artificial diet. J. Asia Pac. Entomol. 1999, 2:169-173.