Enrichment of neonatal rat cardiomyocytes in primary culture facilitates long-term maintenance of contractility in vitro

American Journal of Physiology - Cell Physiology

Volumn 303, Issue 12, 2012, Pages

  Nguyen, Phong D ^a   Hsiao, Sarah T ^a   Sivakumaran, Priyadharshini ^a   Lim, Shiang Y ^a   Dilley, Rodney J ^a,b  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

Cardiomyocyte; Contractility; Enrichment; Tetramethylrhodamine methyl ester perchlorate

Indexed keywords

BIOLOGICAL MARKER; CALCIUM ION; CONNEXIN 43; TETRAMETHYLRHODAMINE METHYL ESTER;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CALCIUM MOBILIZATION; CONTROLLED STUDY; ENRICHMENT CULTURE; EXPERIMENTAL MODEL; FLUORESCENCE ACTIVATED CELL SORTING; HEART MUSCLE CELL; IMMUNOFLUORESCENCE MICROSCOPY; IN VITRO STUDY; MUSCLE FIBER CONTRACTION; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

ANIMALS; ANIMALS, NEWBORN; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; FLOW CYTOMETRY; FLUORESCENT DYES; MYOCARDIAL CONTRACTION; MYOCYTES, CARDIAC; RATS; RATS, SPRAGUE-DAWLEY; RHODAMINES;

RATTUS;

EID: 84871306161     PISSN: 03636143     EISSN: 15221563     Source Type: Journal    
DOI: 10.1152/ajpcell.00449.2011     Document Type: Article

References (38)

1. Angst BD, Khan LU, Severs NJ, Whitely K, Rothery S, Thompson RP, Magee AI, Gourdie RG. Dissociated spatial patterning of gap junctions and cell adhesion junctions during postnatal differentiation of ventricular myocardium. Circ Res 80: 88-94, 1997.
2. Banerjee I, Fuseler JW, Price RL, Borg TK, Baudino TA. Determination of cell types and numbers during cardiac development in the neonatal and adult rat and mouse. Am J Physiol Heart Circ Physiol 293: H1883-H1891, 2007.
3. Banyasz T, Lozinskiy I, Payne CE, Edelmann S, Norton B, Chen B, Chen-Izu Y, Izu LT, Balke CW. Transformation of adult rat cardiac myocytes in primary culture. Exp Physiol 93: 370-382, 2008.
4. Bishop SP, Drummond JL. Surface morphology and cell size measurement of isolated rat cardiac myocytes. J Mol Cell Cardiol 11: 423-433, 1979.
5. Blondel B, Roijen I, Cheneval JP. Heart cells in culture: a simple method for increasing the proportion of myoblasts. Experientia 27: 356-358, 1971.
6. Choi YS, Matsuda K, Dusting GJ, Morrison WA, Dilley RJ. Engineering cardiac tissue in vivo from human adipose-derived stem cells. Biomaterials 31: 2236-2242, 2010.
7. Dedkova EN, Blatter LA. Measuring mitochondrial function in intact cardiac myocytes. J Mol Cell Cardiol 52: 48-61, 2012.
8. Dispersyn GD, Geuens E, Ver Donck L, Ramaekers FC, Borgers M. Adult rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts. Cardiovasc Res 51: 230-240, 2001.
9. Driesen RB, Verheyen FK, Dispersyn GD, Thone F, Lenders MH, Ramaekers FC, Borgers M. Structural adaptation in adult rabbit ventricular myocytes: influence of dynamic physical interaction with fibroblasts. Cell Biochem Biophys 44: 119-128, 2006.
10. Duchen MR, Surin A, Jacobson J. Imaging mitochondrial function in intact cells. Methods Enzymol 361: 353-389, 2003.
11. Feld Y, Melamed-Frank M, Kehat I, Tal D, Marom S, Gepstein L. Electrophysiological modulation of cardiomyocytic tissue by transfected fibroblasts expressing potassium channels: a novel strategy to manipulate excitability. Circulation 105: 522-529, 2002.
12. Haddad J, Decker ML, Hsieh LC, Lesch M, Samarel AM, Decker RS. Attachment and maintenance of adult rabbit cardiac myocytes in primary cell culture. Am J Physiol Cell Physiol 255: C19-C27, 1988.
13. Harary I, Farley B. In vitro studies of single isolated beating heart cells. Science 131: 1674-1675, 1960.
14. Hattori F, Chen H, Yamashita H, Tohyama S, Satoh YS, Yuasa S, Li W, Yamakawa H, Tanaka T, Onitsuka T, Shimoji K, Ohno Y, Egashira T, Kaneda R, Murata M, Hidaka K, Morisaki T, Sasaki E, Suzuki T, Sano M, Makino S, Oikawa S, Fukuda K. Nongenetic method for purifying stem cell-derived cardiomyocytes. Nat Methods 7: 61-66, 2010.
15. Horackova M, Byczko Z. Differences in the structural characteristics of adult guinea pig and rat cardiomyocytes during their adaptation and maintenance in long-term cultures: confocal microscopy study. Exp Cell Res 237: 158-175, 1997.
16. Kabaeva Z, Zhao M, Michele DE. Blebbistatin extends culture life of adult mouse cardiac myocytes and allows efficient and stable transgene expression. Am J Physiol Heart Circ Physiol 294: H1667-H1674, 2008.
17. Karliner JS, Simpson PC, Taylor JE, Honbo N, Woloszyn W. Adrenergic receptor characteristics of cardiac myocytes cultured in serum-free medium: comparison with serum-supplemented medium. Biochem Biophys Res Commun 128: 376-382, 1985.
18. Kawara T, Derksen R, de Groot JR, Coronel R, Tasseron S, Linnenbank AC, Hauer RN, Kirkels H, Janse MJ, de Bakker JM. Activation delay after premature stimulation in chronically diseased human myocardium relates to the architecture of interstitial fibrosis. Circulation 104: 3069-3075, 2001.
19. Khryapenkova TG, Plotnikov EY, Korotetskaya MV, Sukhikh GT, Zorov DB. Heterogeneity of mitochondrial potential as a marker for isolation of pure cardiomyoblast population. Bull Exp Biol Med 146: 506-511, 2008.
20. Konhilas JP, Behunin SM, Lynch RM. Keeping the beat. Focus on "Enrichment of neonatal rat cardiomyocytes in primary culture facilitates long-term maintenance of contractility in vitro. " Am J Physiol Cell Physiol (September 26, 2012). doi:10.1152/ajpcell.00310.2012.
21. LaFramboise WA, Scalise D, Stoodley P, Graner SR, Guthrie RD, Magovern JA, Becich MJ. Cardiac fibroblasts influence cardiomyocyte phenotype in vitro. Am J Physiol Cell Physiol 292: C1799-C1808, 2007.
22. Lemasters JJ, Ramshesh VK. Imaging of mitochondrial polarization and depolarization with cationic fluorophores. Methods Cell Biol 80: 283-295, 2007.
23. Lopez AD, Mathers CD. Measuring the global burden of disease and epidemiological transitions: 2002-2030. Ann Trop Med Parasitol 100: 481-499, 2006.
24. Louault C, Benamer N, Faivre JF, Potreau D, Bescond J. Implication of connexins 40 and 43 in functional coupling between mouse cardiac fibroblasts in primary culture. Biochim Biophys Acta 1778: 2097-2104, 2008.
25. Mathur A, Hong Y, Kemp BK, Barrientos AA, Erusalimsky JD. Evaluation of fluorescent dyes for the detection of mitochondrial membrane potential changes in cultured cardiomyocytes. Cardiovasc Res 46: 126-138, 2000.
26. Pedrotty DM, Klinger RY, Kirkton RD, Bursac N. Cardiac fibroblast paracrine factors alter impulse conduction and ion channel expression of neonatal rat cardiomyocytes. Cardiovasc Res 83: 688-697, 2009.
27. Piper HM, Jacobson SL, Schwartz P. Determinants of cardiomyocyte development in long-term primary culture. J Mol Cell Cardiol 20: 825-835, 1988.
28. Powell T, Sturridge MF, Suvarna SK, Terrar DA, Twist VW. Intact individual heart cells isolated from human ventricular tissue. Br Med J (Clin Res Ed) 283: 1013-1015, 1981.
29. Scaduto RC Jr, Grotyohann LW. Measurement of mitochondrial membrane potential using fluorescent rhodamine derivatives. Biophys J 76: 469-477, 1999.
30. Schafer M, Schafer C, Michael Piper H, Schluter KD. Hypertrophic responsiveness of cardiomyocytes to alpha-or beta-adrenoceptor stimulation requires sodium-proton-exchanger-1 (NHE-1) activation but not cellular alkalization. Eur J Heart Fail 4: 249-254, 2002.
31. Sen A, Dunnmon P, Henderson SA, Gerard RD, Chien KR. Terminally differentiated neonatal rat myocardial cells proliferate and maintain specific differentiated functions following expression of SV40 large T antigen. J Biol Chem 263: 19132-19136, 1988.
32. Simpson DG, Decker ML, Clark WA, Decker RS. Contractile activity and cell-cell contact regulate myofibrillar organization in cultured cardiac myocytes. J Cell Biol 123: 323-336, 1993.
33. Simpson P, Savion S. Differentiation of rat myocytes in single cell cultures with and without proliferating nonmyocardial cells. Cross-striations, ultrastructure, and chronotropic response to isoproterenol. Circ Res 50: 101-116, 1982.
34. Souders CA, Bowers SL, Baudino TA. Cardiac fibroblast: the renaissance cell. Circ Res 105: 1164-1176, 2009.
35. Spach MS, Boineau JP. Microfibrosis produces electrical load variations due to loss of side-to-side cell connections: a major mechanism of structural heart disease arrhythmias. Pacing Clin Electrophysiol 20: 397-413, 1997.
36. Sperelakis N. Cultured heart cell reaggregate model for studying cardiac toxicology. Environ Health Perspect 26: 243-267, 1978.
37. Thompson SA, Copeland CR, Reich DH, Tung L. Mechanical coupling between myofibroblasts and cardiomyocytes slows electric conduction in fibrotic cell monolayers. Circulation 123: 2083-2093, 2011.
38. Zhang Y, Li TS, Lee ST, Wawrowsky KA, Cheng K, Galang G, Malliaras K, Abraham MR, Wang C, Marban E. Dedifferentiation and proliferation of mammalian cardiomyocytes. PLoS One 5: e12559, 2010.