Morphologic and Gene Expression Criteria for Identifying Human Induced Pluripotent Stem Cells

PLoS ONE

Volumn 7, Issue 12, 2012, Pages

  Wakao, Shohei ^a   Kitada, Masaaki ^a   Kuroda, Yasumasa ^a   Ogura, Fumitaka ^a   Murakami, Toru ^a   Niwa, Akira ^b   Dezawa, Mari ^a  

^a TOHOKU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BREAST CANCER RESISTANCE PROTEIN; DNA (CYTOSINE 5) METHYLTRANSFERASE; KRUPPEL LIKE FACTOR 4; MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; RETROVIRUS VECTOR; REX PROTEIN; TRANSCRIPTION FACTOR CDX2; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL CULTURE; CELL DENSITY; CELL NUCLEUS; CELL SIZE; CELL STRUCTURE; CONTROLLED STUDY; CYTOPLASM; DOWN REGULATION; EMBRYOID BODY; FIBROBLAST; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; NONHUMAN; NUCLEOLUS; PLURIPOTENT STEM CELL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOMATIC CELL;

ANIMALS; CELL CULTURE TECHNIQUES; CELL NUCLEOLUS; CELL NUCLEUS; CYTOPLASM; EMBRYONIC STEM CELLS; FIBROBLASTS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GREEN FLUORESCENT PROTEINS; HOMEODOMAIN PROTEINS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; MICE; POLYMERASE CHAIN REACTION; RETROVIRIDAE; RNA; SOXB1 TRANSCRIPTION FACTORS;

IPS;

EID: 84871272087     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0048677     Document Type: Article

References (26)

1. Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel J, et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282: 1145-1147.
2. Cowan CA, Klimanskaya I, McMahon J, Atienza J, Witmyer J, et al. (2004) Derivation of embryonic stem-cell lines from human blastocysts. N Engl J Med 350: 1353-1356.
3. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.
4. Dimos JT, Rodolfa KT, Niakan KK, Weisenthal LM, Mitsumono H, et al. (2008) Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons. Science 321: 1218-1221.
5. Ebert AD, Yu J, Rose FF Jr, Mattis VB, Lorson CL, et al. (2009) Induced pluripotent stem cells from a spinal muscular atrophy patient. Nature 457: 277-280.
6. Masaki H, Ishikawa T, Takahashi S, Okumura M, Sakai N, et al. (2007) Heterogeneity of pluripotent marker gene expression in colonies generated in human iPS cell induction culture. Stem Cell Res 1: 105-115.
7. Huangfu D, Maehr R, Guo W, Eijkelenboom A, Snitow M, et al. (2008) Induction of pluripotent stem cells by defined factors is greatly improved by small-molecule compounds. Nat Biotechnol 26: 795-797.
8. Wernig M, Meissner A, Foreman R, Brambrink T, Ku M, et al. (2007) In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 448: 318-324.
9. Okita K, Ichisaka T, Yamanaka S, (2007) Generation of germline-competent induced pluripotent stem cells. Nature 448: 313-317.
10. Wakao S, Kitada M, Kuroda Y, Shigemoto T, Matsuse D, et al. (2011) Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts. Proc. Natl. Acad. Sci. USA 108: 9875-9880.
11. International Stem Cell Initiative (2007) Adewumi O, Aflatoonian B, Ahrlund-Richter L, Amit M, et al. (2007) Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nat. Biotechnol 25: 803-816.
12. Brimble SN, Zeng X, Weiler DA, Luo Y, Liu Y, et al. (2004) Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9,. Stem Cells Dev 13: 585-597.
13. Sperger JM, Chen X, Draper JS, Antosiewicz JE, Chon CH, et al. (2003) Gene expression patterns in human embryonic stem cells and human pluripotent germ cell tumors. Proc. Natl. Acad. Sci. USA 100: 13350-13355.
14. Ware CB, Nelson AM. Blau CA, (2006) A comparison of NIH-approved human ESC lines. Stem Cells 24: 2677-2684.
15. Chambers I, Colby D, Robertson M, Nicols J, Lee S, et al. (2003) Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell 113: 643-655.
16. Niwa H, Miyazaki J, Smith AG, (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat. Genet 24: 372-376.
17. Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, et al. (2007) Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat. Cell Biol 9: 625-635.
18. Adachi K, Suemori H, Yasuda SY, Nakatsuji N, Kawase E, (2010) Role of SOX2 in maintaining pliripotency of human embryonic stem cells. Genes Cells 15: 455-470.
19. Khoo ML, McQuade LR, Smith MS, Lees JG, Sidhu KS, et al. (2005) Growth and differentiation of embryoid bodies derived from human embryonic stem cells: effect of glucose and basic fibroblast growth factor. Biol. Reprod 73: 1147-1156.
20. Zappone MV, Galli R, Catena R, Meani N, De Biasi S, et al. (2000) Sox2 regulatory sequences direct expression of a b-geo transgene to telencephalic neural stem cells and precursors of the mouse embryo, revealing regionalization of gene expression in CNS stem cells. Development 127: 2367-82.
21. Biernaskie J, Paris M, Morozova O, Fagan BM, Marra M, et al. (2009) SKPs Derive from Hair Follicle Precursors and Exhibit Properties of Adult Dermal Stem Cells. Cell Stem Cell 5: 610-623.
22. Kuroda Y, Kitada M, Wakao S, Nishikawa K, Tanimura Y, et al. (2010) Unique multipotent cells in adult human mesenchymal cell populations. Proc Natl Acad Sci U S A 107: 8639-8643.
23. Chan EM, Ratanasirintrawoot S, Park IH, Manos PD, Loh YH, et al. (2009) Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells. Nat. Biotechnol 27: 1033-1037.
24. Spassky N, Goujet-Zalc C, Parmantier E, Olivier C, Martinez S, et al. (1998) Multiple restricted origin of oligodendrocytes. J Neurosci 18: 8331-8343.
25. Spassky N, Olivier C, Perez-Villegas E, Goujet-Zalc C, Martinez S, et al. (2000) Single or multiple oligodendroglial lineages: a controversy. Glia 29: 143-148.
26. Ito M, Hiramatsu H, Kobayashi K, Suzue K, Kawahata M, et al. (2002) NOD/SCID/gamma(c)(null) mouse: an excellent recipient mouse model for engraftment of human cells. Blood 1: 3175-3182.