Evidence for two promoters internal to the alginate biosynthesis operon in Pseudomonas aeruginosa

Current Microbiology

Volumn 65, Issue 6, 2012, Pages 770-775

  Paletta, Janice L ^a,b   Ohman, Dennis E ^a,b  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALGINIC ACID; BETA GALACTOSIDASE; EPIMERASE;

ACETYLATION; ALGA GENE; ALGD GENE; ALGF GENE; ALGG GENE; ALGI GENE; ALGJ GENE; ALGX GENE; ARTICLE; BACTERIAL GENE; CARBOHYDRATE SYNTHESIS; EPIMERIZATION; GENE ACTIVITY; GENE CONTROL; GENE IDENTIFICATION; GENE SEQUENCE; GENETIC TRANSCRIPTION; LACZ GENE; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PSEUDOMONAS AERUGINOSA; REPORTER GENE; SEQUENCE ANALYSIS; TRANSCRIPTION INITIATION SITE;

ACETYLATION; ALGINATES; BACTERIAL PROTEINS; BASE SEQUENCE; BETA-GALACTOSIDASE; CULTURE MEDIA; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; MOLECULAR SEQUENCE DATA; OPERON; PROMOTER REGIONS, GENETIC; PSEUDOMONAS AERUGINOSA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

PSEUDOMONAS AERUGINOSA;

EID: 84871266602     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-012-0228-y     Document Type: Article

References (23)

1. Boucher JC, Schurr MJ, Deretic V (2000) Dual regulation of mucoidy in Pseudomonas aeruginosa and sigma factor antagonism. Mol Microbiol 36:341-351
2. Chitnis CE, Ohman DE (1990) Cloning of Pseudomonas aeruginosa algG, which controls alginate structure. J Bacteriol 172: 2894-2900
3. Chitnis CE, Ohman DE (1993) Genetic analysis of the alginate biosynthetic gene cluster of Pseudomonas aeruginosa shows evidence of an operonic structure. Mol Microbiol 8:583-593
4. DeVries CA, Ohman DE (1994) Mucoid-to-nonmucoid conversion in alginate-producing Pseudomonas aeruginosa often results from spontaneous mutations in algT, encoding a putative alternate sigma factor, and shows evidence for autoregulation. J Bacteriol 176:6677-6687
5. Dische Z (1949) Spectrophotometric method for the determination of free pentose and pentose in nucleotides. J Biol Chem 181:379-392
6. Ertesvåg H, Høidal HK, Schjerven H, Svanem BIG, Valla S (1999) Mannuronan C-5-epimerases and their application for in vitro and in vivo design of new alginates useful in biotechnology. Metab Eng 1:262-269
7. Franklin MJ, Chitnis CE, Gacesa P, Sonesson A, White DC, Ohman DE (1994) Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase. J Bacteriol 176:1821-1830
8. Franklin MJ, Ohman DE (2002) Mutant analysis and cellular localization of the AlgI, AlgJ and AlgF proteins required for O acetylation of alginate in Pseudomonas aeruginosa. J Bacteriol 184:3000-3007
9. Hestrin S (1949) The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine, and its analytical application. J Biol Chem 180:249-261
10. Lee HJ, Jeon HJ, Ji SC, Yun SH, Lim HM (2008) Establishment of an mRNA gradient depends on the promoter: an investigation of polarity in gene expression. J Mol Biol 378:318-327
11. Marty N, Dournes JL, Chabanon G, Montrozier H (1992) Influence of nutrient media on the chemical composition of the exopolysaccharide from mucoid and non-mucoid Pseudomonas aeruginosa. FEMS Microbiol Lett 77:35-44
12. Mian FA, Jarman TR, Righelato RC (1978) Biosynthesis of exopolysaccharide by Pseudomonas aeruginosa. J Bacteriol 134:418-422
13. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
14. Monday SR, Schiller NL (1996) Alginate synthesis in Pseudomonas aeruginosa: the role of AlgL (alginate lyase) and AlgX. J Bacteriol 178:625-632
15. Nunez C, Moreno S, Soberon-Chavez G, Espin G (1999) The Azotobacter vinelandii response regulator AlgR is essential for cyst formation. J Bacteriol 181:141-148
16. Pena C, Hernandez L, Galindo E (2006) Manipulation of the acetylation degree of Azotobacter vinelandii alginate by supplementing the culture medium with 3-(N-morpholino)-propanesulfonic acid. Lett Appl Microbiol 43:200-204
17. Pier GB, Coleman F, Grout M, Franklin M, Ohman DE (2001) Role of alginate O acetylation in resistance of mucoid Pseudomonas aeruginosa to opsonic phagocytosis. Infect Immun 69:1895-1901
18. Rehm BHA, Valla S (1997) Bacterial alginates: biosynthesis and applications. Appl Microbiol Biotechnol 48:281-288
19. Singh S (1987) Mucoid strains of Pseudomonas aeruginosa are devoid of mannuronan C-5 epimerase. Microbios 51:7-13
20. Steigedal M, Sletta H, Moreno S, Maerk M, Christensen BE, Bjerkan T, Ellingsen TE, Espin G, Ertesvag H, Valla S (2008) The Azotobacter vinelandii AlgE mannuronan C-5-epimerase family is essential for the in vivo control of alginate monomer composition and for functional cyst formation. Environ Microbiol 10:1760-1770
21. Suh SJ, Silo-Suh LA, Ohman DE (2004) Development of tools for the genetic manipulation of Pseudomonas aeruginosa. J Microbiol Methods 58:203-212
22. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680
23. Vazquez A, Moreno S, Guzmán J, Alvarado A, Espi'n G (1999) Transcriptional organization of the Azotobacter vinelandii algGXLVIFA genes: characterization of algF mutants. Gene 232:217-222