Comparison of proliferative capacity of genetically-engineered pig and human corneal endothelial cells

Ophthalmic Research

Volumn 49, Issue 3, 2013, Pages 127-138

  Fujita, Minoru ^a   Mehra, Ruhina ^a   Lee, Seung Eun ^a   Roh, Danny S ^b   Long, Cassandra ^a   Funderburgh, James L ^b   Ayares, David L ^c   Cooper, David K C ^a   Hara, Hidetaka ^a  

^a UNIVERSITY OF PITTSBURGH MEDICAL CENTER   (United States)

^b University of Pittsburgh School of Medicine   (United States)

^c REVIVICOR INC   (United States)

Author keywords

Corneal endothelial cells; Endothelial keratoplasty; Genetically engineered; Pig; Proliferative capacity; Xenotransplantation

Indexed keywords

DECAY ACCELERATING FACTOR; MEMBRANE COFACTOR PROTEIN; LIPOCORTIN 5;

ADULT; AGED; ANIMAL CELL; ARTICLE; CELL COUNT; CELL CULTURE; CELL DEATH; CELL METABOLISM; CELL PROLIFERATION; CELL VIABILITY; CORNEA ENDOTHELIUM; GENETIC ENGINEERING; HUMAN; HUMAN CELL; HUMAN TISSUE; NEWBORN; NONHUMAN; PRIORITY JOURNAL; ANIMAL CELL CULTURE; BRDU ASSAY; CELL COUNTING; COMPARATIVE STUDY; CORNEA CELL; ENDOTHELIUM CELL; FEMALE; GENETICALLY ENGINEERED PIG CORNEAL ENDOTHELIAL CELL; HUMAN CELL CULTURE; HUMAN CORNEAL ENDOTHELIAL CELL; IN VITRO STUDY; MALE; MIDDLE AGED; MTT ASSAY; PIG CORNEAL ENDOTHELIAL CELL; RIBOSWITCH; TRANSGENIC PIG; WILD TYPE; YOUNG ADULT;

ADULT; ANIMALS; ANIMALS, GENETICALLY MODIFIED; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; ENDOTHELIAL CELLS; ENDOTHELIUM, CORNEAL; FEMALE; HUMANS; MALE; MIDDLE AGED; SWINE; TETRAZOLIUM SALTS; THIAZOLES;

EID: 84871243242     PISSN: 00303747     EISSN: 14230259     Source Type: Journal    
DOI: 10.1159/000342978     Document Type: Article

References (37)

1. Joyce NC: Proliferative capacity of the corneal endothelium. Prog Retin Eye Res 2003; 22: 359-389.
2. Glasser DB: Changing trends in keratoplasty. Am J Ophthalmol 2011; 151: 394-396.
3. Engelmann K, Bednarz J, Valtink M: Prospects for endothelial transplantation. Exp Eye Res 2004; 78: 573-578.
4. Dapena I, Ham L, Melles GR: Endothelial keratoplasty: DSEK/DSAEK or DMEK - the thinner the better? Curr Opin Ophthalmol 2009; 20: 299-307.
5. Terry MA, Shamie N, Chen ES, Hoar KL, Phillips PM, Friend DJ: Endothelial keratoplasty: the influence of preoperative donor endothelial cell densities on dislocation, primary graft failure, and 1-year cell counts. Cornea 2008; 27: 1131-1137.
6. Joyce NC, Meklir B, Joyce SJ, Zieske JD: Cell cycle protein expression and proliferative status in human corneal cells. Invest Ophthalmol Vis Sci 1996; 37: 645-655.
7. Sumide T, Nishida K, Yamato M, Ide T, Hayashida Y, Watanabe K, Yang J, Kohno C, Kikuchi A, Maeda N, Watanabe H, Okano T, Tano Y: Functional human corneal endothelial cell sheets harvested from temperature-responsive culture surfaces. FASEB J 2006; 20: 392-394.
8. Koizumi N, Sakamoto Y, Okumura N, Okahara N, Tsuchiya H, Torii R, Cooper LJ, Ban Y, Tanioka H, Kinoshita S: Cultivated corneal endothelial cell sheet transplantation in a primate model. Invest Ophthalmol Vis Sci 2007; 48: 4519-4526.
9. Peh GS, Beuerman RW, Colman A, Tan DT, Mehta JS: Human corneal endothelial cell expansion for corneal endothelium transplantation: an overview. Transplantation 2011; 91: 811-819.
10. Hara H, Cooper DK: Xenotransplantation - the future of corneal transplantation? Cornea 2011; 30: 371-378.
11. Hara H, Cooper DK: The immunology of corneal xenotransplantation: a review of the literature. Xenotransplantation 2010; 17: 338-349.
12. Kim MK, Wee WR, Park CG, Kim SJ: Xenocorneal transplantation. Curr Opin Organ Transplant 2011; 16: 231-236.
13. Hara H, Koike N, Long C, Piluek J, Roh DS, Sundarraj N, Funderburgh JL, Mizuguchi Y, Isse K, Phelps CJ, Ball SF, Ayares DL, Cooper DK: Initial in vitro investigation of the human immune response to corneal cells from genetically-engineered pigs. Invest Ophthalmol Vis Sci 2011; 52: 5278-5286.
14. Hara H, Long C, Lin YJ, Tai HC, Ezzelarab M, Ayares D, Cooper DK: In vitro investigation of pig cells for resistance to human antibody- mediated rejection. Transpl Int 2008; 21: 1163-1174.
15. Shimizu K, Koga H, Takeda M, Mikami Y, Tsunoda T, Iida M, Haruma K: Suddenly accelerated growth of gastrointestinal stromal tumor of remnant stomach. Dig Dis Sci 2003; 48: 1106-1109.
16. Nucci P, Brancato R, Mets MB, Shevell SK: Normal endothelial cell density range in childhood. Arch Ophthalmol 1990; 108: 247-248.
17. Bourne WM, Nelson LR, Hodge DO: Central corneal endothelial cell changes over a tenyear period. Invest Ophthalmol Vis Sci 1997; 38: 779-782.
18. Baserga R: The relationship of the cell cycle to tumor growth and control of cell division: a review. Cancer Res 1965; 25: 581-595.
19. Konomi K, Zhu C, Harris D, Joyce NC: Comparison of the proliferative capacity of human corneal endothelial cells from the central and peripheral areas. Invest Ophthalmol Vis Sci 2005; 46: 4086-4091.
20. Chen J, Guerriero E, Lathrop K, SundarRaj N: Rho/ROCK signaling in regulation of corneal epithelial cell cycle progression. Invest Ophthalmol Vis Sci 2008; 49: 175-183.
21. Dolbeare F, Gratzner H, Pallavicini MG, Gray JW: Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci USA 1983; 80: 5573-5577.
22. Lee WB, Jacobs DS, Musch DC, Kaufman SC, Reinhart WJ, Shtein RM: Descemet's stripping endothelial keratoplasty: safety and outcomes: a report by the American Academy of Ophthalmology. Ophthalmology 2009; 116: 1818-1830.
23. Balachandran C, Ham L, Verschoor CA, Ong TS, van der Wees J, Melles GR: Spontaneous corneal clearance despite graft detachment in Descemet membrane endothelial keratoplasty. Am J Ophthalmol 2009; 148: 227-234, e221.
24. Mimura T, Yamagami S, Yokoo S, Usui T, Tanaka K, Hattori S, Irie S, Miyata K, Araie M, Amano S: Cultured human corneal endothelial cell transplantation with a collagen sheet in a rabbit model. Invest Ophthalmol Vis Sci 2004; 45: 2992-2997.
25. Lai JY, Chen KH, Hsiue GH: Tissue-engineered human corneal endothelial cell sheet transplantation in a rabbit model using functional biomaterials. Transplantation 2007; 84: 1222-1232.
26. Koizumi N, Sakamoto Y, Okumura N, Tsuchiya H, Torii R, Cooper LJ, Ban Y, Tanioka H, Kinoshita S: Cultivated corneal endothelial transplantation in a primate: possible future clinical application in corneal endothelial regenerative medicine. Cornea 2008; 27(suppl 1):S48-S55.
27. Okumura N, Koizumi N, Ueno M, Sakamoto Y, Takahashi H, Hirata K, Torii R, Hamuro J, Kinoshita S: Enhancement of corneal endothelium wound healing by Rho-associated kinase (ROCK) inhibitor eye drops. Br J Ophthalmol 2011; 95: 1006-1009.
28. Okumura N, Ueno M, Koizumi N, Sakamoto Y, Hirata K, Hamuro J, Kinoshita S: Enhancement on primate corneal endothelial cell survival in vitro by a ROCK inhibitor. Invest Ophthalmol Vis Sci 2009; 50: 3680-3687.
29. Singh G, Bohnke M, Draeger J: Endothelial wound healing in organ-cultured pig corneae after mechanical trauma. Fortschr Ophthalmol 1984; 81: 293-295.
30. Zhu C, Joyce NC: Proliferative response of corneal endothelial cells from young and older donors. Invest Ophthalmol Vis Sci 2004; 45: 1743-1751.
31. Engelmann K, Friedl P: Growth of human corneal endothelial cells in a serum-reduced medium. Cornea 1995; 14: 62-70.
32. Ishino Y, Sano Y, Nakamura T, Connon CJ, Rigby H, Fullwood NJ, Kinoshita S: Amniotic membrane as a carrier for cultivated human corneal endothelial cell transplantation. Invest Ophthalmol Vis Sci 2004; 45: 800-806.
33. Li W, Sabater AL, Chen YT, Hayashida Y, Chen SY, He H, Tseng SC: A novel method of isolation, preservation, and expansion of human corneal endothelial cells. Invest Ophthalmol Vis Sci 2007; 48: 614-620.
34. Hara H, Crossley T, Witt W, Long C, Fang J, Ezzelarab M, Starzl TE, Ayares D, Cooper DK, Dai Y: Dominant-negative CIITA transgenic pigs - effect on the human anti-pig T cell immune response and immune status. Am J Transplant 2010; 10: 187.
35. Phelps CJ, Ball SF, Vaught TD, Vance AM, Mendicino M, Monahan JA, Walters AH, Wells KD, Dandro AS, Ramsoondar JJ, Cooper DK, Ayares DL: Production and characterization of transgenic pigs expressing porcine CTLA4-Ig. Xenotransplantation 2009; 16: 477-485.
36. Koshika T, Phelps C, Fang J, Lee SE, Fujita M, Ayares D, Cooper DK, Hara H: Relative efficiency of porcine and human cytotoxic Tlymphocyte antigen 4 immunoglobulin in inhibiting human CD4(+) T-cell responses co-stimulated by porcine and human B7 molecules. Immunology 2011; 134: 386-397.
37. Koizumi N, Okumura N, Kinoshita S: Development of new therapeutic modalities for corneal endothelial disease focused on the proliferation of corneal endothelial cells using animal models. Exp Eye Res 2012; 95: 60-67.