A validated and rapid high-performance liquid chromatography method for the quantification of conversion of radio-labelled sex steroids

Hormone Molecular Biology and Clinical Investigation

Volumn 3, Issue 1, 2010, Pages 375-381

  Sivik, Tove ^a   Jansson, Agneta ^a   Vikingsson, Svante ^a   Green, Henrik ^a  

^a LINKÖPING UNIVERSITY   (Sweden)

Author keywords

17 hydroxysteroid dehydrogenase; high performance liquid chromatography; MCF7; radiochemical detection; steroid conversion; T 47D

Indexed keywords

ANDROGEN; ANDROSTENEDIONE; ESTRADIOL; ESTROGEN; ESTRONE; SEX HORMONE; SODIUM HYDROXIDE; TESTOSTERONE; TESTOSTERONE 17BETA DEHYDROGENASE; ZINC SULFATE;

ARTICLE; BREAST CANCER; ENDOMETRIUM CANCER; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOTOPE LABELING; PRECIPITATION; PRIORITY JOURNAL; PROSTATE CANCER; QUALITY CONTROL; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 84871223880     PISSN: 18681883     EISSN: 18681891     Source Type: Journal    
DOI: 10.1515/HMBCI.2010.038     Document Type: Article

References (22)

1. Day JM., Tutill HJ., Purohit A., Reed MJ. Design and validation of specific inhibitors of 17Β-hydroxysteroid dehydrogenases for therapeutic application in breast and prostate cancer., and in endo-metriosis. Endocr Relat Cancer 2008;15:665-92.
2. Miettinen MM., Poutanen MH., Vihko RK. Characterization of estrogen-dependent growth of cultured MCF-7 human breast-cancer cells expressing 17Β-hydroxysteroid dehydrogenase type 1. Int J Cancer 1996;68:600-4.
3. Gunnarsson C., Olsson BM., Stål O., Arnesson LG., Nordenskjold B., Nordenskjöld K., Malmstrom A., Bång H., Kallström AC., Einarsson E., Norberg B., Skoog P., Henning A., Sundquist M., Tejler G. Abnormal expression of 17Β-hydroxysteroid dehydro-genases in breast cancer predicts late recurrence. Cancer Res 2001;61:8448-51.
4. Ito K., Utsunomiya H., Suzuki T., Saitou S., Akahira JI., Okamura K., Yaegashi N., Sasano H. 17Β-Hydroxysteroid dehydrogenases in human endometrium and its disorders. Mol Cell Endocrinol 2006;248:136-40.
5. Labrie F., Dupont A., Simard J., Luu-The V., Belanger A. Intracri-nology: the basis for the rational design of endocrine therapy at all stages of prostate cancer. Eur Urol 1993;24(Suppl 2): 94-105.
6. Oduwole OO., Li Y., Isomaa VV., Mäntyniemi A., Pulkka AE., Soini Y., Vihko PT. 17Β-Hydroxysteroid dehydrogenase type 1 is an independent prognostic marker in breast cancer. Cancer Res 2004;64:7604-9.
7. Jansson AK., Gunnarsson C., Cohen M., Sivik T., Ståi O. 17Β-Hydroxysteroid dehydrogenase 14 affects estradiol levels in breast cancer cells and is a prognostic marker in estrogen receptor-positive breast cancer. Cancer Res 2006;66:11471-7.
8. Geisler J., Berntsen H., Lonning PE. A novel HPLC-RIA method for the simultaneous detection of estrone., estradiol and estrone sulphate levels in breast cancer tissue. J Steroid Biochem Mol Biol 2000;72:259-64.
9. Day JM., Foster PA., Tutill HJ., Parsons MF., Newman SP., Chan-der SK., Allan GM., Lawrence HR., Vicker N., Potter BV., Reed MJ., Purohit A. 17Β-Hydroxysteroid dehydrogenase type 1, and not type 12, is a target for endocrine therapy of hormone-dependent breast cancer. Int J Cancer 2008;122:1931-40.
10. Sherbet DP., Guryev OL., Papari-Zareei M., Mizrachi D., Ram-bally S., Akbar S., Auchus RJ. Biochemical factors governing the steady-state estrone/estradiol ratios catalyzed by human 17Β-hydroxysteroid dehydrogenases types 1 and 2 in HEK-293 cells. Endocrinology 2009;150:4154-62.
11. Penning TM., Burczynski ME., Jez JM., Hung CF., Lin HK., Ma HC., Moore M., Palackal N., Ratnam K. Human 3α-hydroxys-teroid dehydrogenase isoforms (AKR1C1-AKR1C4) of the aldo-keto reductase superfamily: functional plasticity and tissue distribution reveals roles in the inactivation and formation of male and female sex hormones. Biochem J 2000;351:67-77.
12. Luu-The V., Tremblay P., Labrie F. Characterization of type 12 17Β-hydroxysteroid dehydrogenase., an isoform of type 3 17Β-hydroxysteroid dehydrogenase responsible for estradiol formation in women. Mol Endocrinol 2006;20:437-43.
13. Lukacik P., Keller B., Bunkoczi G., Kavanagh KL., Lee WH., Adamski J., Oppermann U. Structural and biochemical characterization of human orphan DHRS10 reveals a novel cytosolic enzyme with steroid dehydrogenase activity. Biochem J 2007; 402:419-27.
14. Shen Z., Saloniemi T., Ronnblad A., Jarvensivu P., Pakarinen P., Poutanen M. Sex steroid-dependent and-independent action of hydroxysteroid (17Β) dehydrogenase 2: evidence from transgenic female mice. Endocrinology 2009;150:4941-9.
15. Miettinen MM., Mustonen MV., Poutanen MH., Isomaa VV., Vihko RK. Human 17Β-hydroxysteroid dehydrogenase type 1 and type 2 isoenzymes have opposite activities in cultured cells and characteristic cell-and tissue-specific expression. Biochem J 1996;314:839-45.
16. Schmidt M., Hartung R., Capellino S., Cutolo M., Pfeifer-Leeg A., Straub RH. Estrone/17Β-estradiol conversion to., and tumor necrosis factor inhibition by., estrogen metabolites in synovial cells of patients with rheumatoid arthritis and patients with osteoarthritis. Arthritis Rheum 2009;10:2913-22.
17. Thompson EA Jr., Siiteri PK. Utilization of oxygen and reduced nicotinamide adenine dinucleotide phosphate by human placen-tal microsomes during aromatization of androstenedione. J Biol Chem 1974;249:5364-72.
18. Chetrite GS., Kloosterboer HJ., Philippe JC., Pasqualini JR. Effects of Org OD14 (Livial®) and its metabolites on 17Β-hydroxysteroid dehydrogenase activity in hormone-dependent MCF-7 and T-47D breast cancer cells. Anticancer Res 1999; 19:261-7.
19. Day JM., Tutill HJ., Newman SP., Purohit A., Lawrence HR., Vicker N., Potter BV., Reed MJ. 17Β-Hydroxysteroid dehydrogenase type 1 and type 2: association between mRNA expression and activity in cell lines. Mol Cell Endocrinol 2006;248:246-9.
20. Kokko T., Kokko L., Lovgren T., Soukka T. Homogeneous noncompetitive immunoassay for 17b-estradiol based on fluorescence resonance energy transfer. Anal Chem 2007;79:5935-40.
21. Kokko L., Jaakohuhta S., Lindroos P., Soukka T. Improved homogeneous proximity-based screening assay of potential inhibitors of 17Β-hydroxysteroid dehydrogenases. Assay Drug Dev Tech-nol 2006;4:671-8.
22. Delvoux B., Husen B., Aldenhoff Y., Koole L., Dunselman G., Thole H., Groothuis P. A sensitive HPLC method for the assessment of metabolic conversion of estrogens. J Steroid Biochem Mol Biol 2007;104:246-51.