Establishment of a bacterial expression system and immunoassay platform for the major capsid protein of HcRNAV, a dinoflagellate-infecting RNA virus

Microbes and Environments

Volumn 27, Issue 4, 2012, Pages 483-489

  Wada, Kei ^a,c   Kimura, Kei ^b   Hasegawa, Akifumi ^a   Fukuyama, Keiichi ^a   Nagasaki, Keizo ^b,d  

^a OSAKA UNIVERSITY   (Japan)

^b FISHERIES RESEARCH AGENCY   (Japan)

^c UNIVERSITY OF MIYAZAKI   (Japan)

^d JAPAN FISHERIES RESEARCH AND EDUCATION AGENCY   (Japan)

Author keywords

Bacterial expression; Heterocapsa circularisquama RNA virus; Major capsid protein; Polyclonal antibody

Indexed keywords

CAPSID PROTEIN; RECOMBINANT PROTEIN; VIRUS ANTIBODY; VIRUS RNA;

ANIMAL; ARTICLE; BIOSYNTHESIS; CODON; DINOFLAGELLATE; ESCHERICHIA COLI; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENE VECTOR; GENETICS; IMMUNIZATION; IMMUNOLOGY; METABOLISM; RABBIT; RNA VIRUS; VIROLOGY;

ANIMALS; ANTIBODIES, VIRAL; CAPSID PROTEINS; CODON; DINOFLAGELLIDA; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; IMMUNIZATION; MICROSCOPY, FLUORESCENCE; RABBITS; RECOMBINANT PROTEINS; RNA VIRUSES; RNA, VIRAL;

BACTERIA (MICROORGANISMS); DINOPHYCEAE; ESCHERICHIA COLI; HETEROCAPSA CIRCULARISQUAMA; HETEROCAPSA CIRCULARISQUAMA RNA VIRUS; MIRIDAE; ORYCTOLAGUS CUNICULUS; RNA VIRUSES;

EID: 84871139240     PISSN: 13426311     EISSN: 13474405     Source Type: Journal    
DOI: 10.1264/jsme2.ME12046     Document Type: Article

References (34)

1. Attoui, H., F. Mohd Jaafar, M. Belhouchet, P. de Micco, X. de Lamballerie, and C.P. Brussaard. 2006. Micromonas pusilla reovirus: a new member of the family Reoviridae assigned to a novel proposed genus (Mimoreovirus). J. Gen. Virol. 87:1375-1383.
2. Bachvaroff, T.R., G.T. Concepcion, C.R. Rogers, E.M. Herman, and C.F. Delwiche. 2004. Dinoflagellate expressed sequence tag data indicate massive transfer of chloroplast genes to the nuclear genome. Protist 155:65-78.
3. Bisgrove, S.R., and D.L. Kropf. 2004. Cytokinesis in brown algae: studies of asymmetric division in fucoid zygotes. Protoplasma 223:163-173.
4. Brussaard, C.P., A.A. Noordeloos, R.A. Sandaa, M. Heldal, and G. Bratbak. 2004. Discovery of a dsRNA virus infecting the marine photosynthetic protist Micromonas pusilla. Virology 319:280-291.
5. Cherrier, M.V., V.A. Kostyuchenko, C. Xiao, et al. 2009. An icosahedral algal virus has a complex unique vertex decorated by a spike. Proc. Natl. Acad. Sci. U.S.A. 106:11085-11089.
6. Horiguchi, T. 1995. Heterocapsa circularisquama sp. nov. (Peridiniales, Dinophyceae): A new marine dinoflagellate causing mass mortality of bivalves in Japan. Phycol. Res. 43:129-136
7. McEwan, M., R. Humayun, C.H. Slamovits, and P.J. Keeling. 2008. Nuclear genome sequence survey of the Dinoflagellate Heterocapsa triquetra. J. Eukaryot. Microbiol. 55:530-535.
8. Michaux, C., N.C. Pomroy, and G.G. Prive. 2008. Refolding SDS-denatured proteins by the addition of amphipathic cosolvents. J. Mol. Biol. 375:1477-1488.
9. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
10. Lang, A. S., A.I. Culley, and C.A. Suttle. 2004. Genome sequence and characterization of a virus (HaRNAV) related to picorna-like viruses that infects the marine toxic bloom-forming alga Heterosigma akashiwo. Virology 320:206-217.
11. Maier, I., D.G. Müller, and C. Katsaros. 2002. Entry of the DNA virus, Ectocarpus fasciculatus virus type 1 (Phycodnaviridae), into host cell cytosol and nucleus. Phycol. Res. 50:227-231.
12. Meints, R.H., D.E. Burbank, J.L. Van Etten, and D.T. Lamport. 1988. Properties of the Chlorella receptor for the virus PBCV-1. Virology 164:15-21.
13. Miller, J.L., J. Woodward, S. Chen, et al. 2011. Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by electron cryo-microscopy. J. Gen. Virol. 92:1960-1970.
14. Motomura, T., and T. Hishinuma. 1997. Methods for immuno-fluorescence observation on the algal cytoskeleton. Jpn. J. Phycol. 45:175-181.
15. Nagasaki, K. 2008. Dinoflagellates, diatoms, and their viruses. J. Microbiol. 46:235-243.
16. Nagasaki, K., Y. Shirai, Y. Takao, H. Mizumoto, K. Nishida, and Y. Tomaru. 2005. Comparison of genome sequences of single-stranded RNA viruses infecting the bivalve-killing dinoflagellate Heterocapsa circularisquama. Appl. Environ. Microbiol. 71:8888-8894.
17. Nagasaki, K., Y. Tomaru, N. Katanozaka, Y. Shirai, K. Nishida, S. Itakura, and M. Yamaguchi. 2004. Isolation and characterization of a novel single-stranded RNA virus infecting the bloom-forming diatom Rhizosolenia setigera. Appl. Environ. Microbiol. 70:704-711.
18. Nagasaki, K., Y. Tomaru, K. Nakanishi, N. Hata, N. Katanozaka, and M. Yamaguchi. 2004. Dynamics of Heterocapsa circularisquama (Dinophyceae) and its viruses in Ago Bay, Japan. Aquat. Microb. Ecol. 34:219-216.
19. Onimatsu, H., K. Suganuma, S. Uenoyama, and T. Yamada. 2006. C-terminal repetitive motifs in Vp130 present at the unique vertex of the Chlorovirus capsid are essential for binding to the host Chlorella cell wall. Virology 353:433-442.
20. Ortiz, M.L., M. Calero, C. Fernandez Patron, C.F. Patron, L. Castellanos, and E. Mendez. 1992. Imidazole-SDS-Zn reverse staining of proteins in gels containing or not SDS and micro sequence of individual unmodified electroblotted proteins. FEBS Lett. 296:300-304.
21. Patron, N.J., R.F. Waller, and P.J. Keeling. 2006. A tertiary plastid uses genes from two endosymbionts. J. Mol. Biol. 357:1373-1382.
22. Patron, N.J., R.F. Waller, J.M. Archibald, and P.J. Keeling. 2005. Complex protein targeting to dinoflagellate plastids. J. Mol. Biol. 348:1015-1024.
23. Raab, D., M. Graf, F. Notka, T. Schödl, and R. Wagner. 2010. The GeneOptimizer Algorithm: using a sliding window approach to cope with the vast sequence space in multiparameter DNA sequence optimization. Syst. Synth. Biol. 4:215-225.
24. Reisser, W., and E. Kapaun. 1991. Entry of a Chlorella-virus into its host-cell. J. Phycol. 27:609-613.
25. Roberts, K.R., J.E. Lemoine, R.M. Schneider, and M.A. Farmer. 1988. The microtubular cytoskeleton of three dinoflagellates: an immunofluorescence study. Protoplasma 144:68-71.
26. Shirai, Y., Y. Takao, H. Mizumoto, Y. Tomaru, D. Honda, and K. Nagasaki. 2006. Genomic and phylogenetic analysis of a single-stranded RNA virus infecting Rhizosolenia setigera (Stramenopiles: Bacillariophyceae). J. Mar. Biol. Ass. U.K. 86:475-483.
27. Shirai, Y., Y. Tomaru, Y. Takao, H. Suzuki, T. Nagumo, and K. Nagasaki. 2008. Isolation and characterization of a single-stranded RNA virus infecting the marine planktonic diatom Chaetoceros tenuissimus Meunier. Appl. Environ. Microbiol. 74:4022-4027.
28. Tai, V., J.E. Lawrence, A.S. Lang, A.M. Chan, A.I. Culley, and C.A. Suttle. 2003. Characterization of HaRNAV, a single-stranded RNA virus causing lysis of Heterosigma akashiwo (Raphidophyceae). J. Phycol. 39:343-352.
29. Takao, Y., K. Mise, K. Nagasaki, T. Okuno, and D. Honda. 2006. Complete nucleotide sequence and genome organization of a single-stranded RNA virus infecting the marine fungoid protist Schizochytrium sp. J. Gen. Virol. 87:723-733.
30. Takao, Y., K. Nagasaki, K. Mise, T. Okuno, and D. Honda. 2005. Isolation and characterization of a novel single-stranded RNA virus infectious to a marine fungoid protist, Schizochytrium sp. (Thraustochytriaceae, Labyrinthulea). Appl. Environ. Microbiol. 71:4516-4522.
31. Tomaru, Y., N. Katanozaka, K. Nishida, Y. Shirai, K. Tarutani, M. Yamaguchi, and K. Nagasaki. 2004. Isolation and characterization of two distinct types of HcRNAV, a single-stranded RNA virus infecting the bivalve-killing microalga Heterocapsa circularisquama. Aquat. Microb. Ecol. 34:207-218.
32. Tomaru, Y., Y. Takao, H. Suzuki, T. Nagumo, and K. Nagasaki. 2009. Isolation and characterization of a single-stranded RNA virus infecting the bloom-forming diatom Chaetoceros socialis. Appl. Environ. Microbiol. 75:2375-2381.
33. Tomaru, Y., K. Toyoda, K. Kimura, N. Hata, M. Yoshida, and K. Nagasaki. 2012. First evidence for the existence of pennate diatom viruses. ISME J. doi:10.1038/ismej.2011.207.
34. Tomaru, Y., H. Mizumoto, and K. Nagasaki. 2009. Virus resistance of the bloom-forming dinoflagellate Heterocapsa circularisquama to its single-stranded RNA virus infection. Environ. Microbiol. 11:2915-2923.