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Volumn 338, Issue 6113, 2012, Pages 1458-1461

Rapid folding of DNA into nanoscale shapes at constant temperature

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

EID: 84871091378     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1229919     Document Type: Article
Times cited : (239)

References (32)
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    • Han, D.1
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    • note
    • In the fluorometric experiments, trace amounts of the organic dye SYBR green (SG) were added to one- pot self-assembly reaction mixtures (18). The use of low concentrations of SG corresponding to one SG molecule per ~900 possible DNA base pairs in solution ensured negligible perturbation of the DNA hybridization processes. The fluorescence brightness of SG is enhanced upon intercalation into dsDNA domains (29); recording the SG fluorescence intensity can thus provide a measure for the evolution of the overall content of DNA base pairs in solution. A specific signal for the formation of object-related DNA base pairs upon incubation for time t at temperature T is obtained by comparison with reference reactions (18). For templated DNA objects-i.e., objects that form by staple DNA strand-assisted folding of a much longer scaffold DNA template molecule (4)-reference reactions were prepared that included the respective set of staple DNA oligonucleotides but lacked the scaffold DNA strand. Cryogenic shock-freezing of a reaction mixture in liquid nitrogen quenched folding and unfolding processes and preserved the state of the reaction at the time and temperature of freezing (18). After quick thawing, the solution content was analyzed using agarose gel electrophoresis and/or direct imaging with negative-stain TEM. Gel electrophoresis and TEM imaging were performed as previously described (30). Excess staple strands were removed by molecular weight cut-off filtration with 100-kD spin filters. Objects were designed using caDNAno (31); models were computed using CanDo (30, 32).
  • 18
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    • Materials and methods are available as supplementary materials on
    • Materials and methods are available as supplementary materials on Science Online.
    • Science Online
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    • J. Zheng et al., Nature 461, 74 (2009).
    • (2009) Nature , vol.461 , pp. 74
    • Zheng, J.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.