Cytological specimens obtained by endobronchial ultrasound-guided transbronchial needle aspiration: Sample handling and role of rapid on-site evaluation

Annales de Pathologie

Volumn 32, Issue 6, 2012, Pages

  Yasufuku, Kazuhiro ^a   Feith, Jocelyne Fleury ^b,c  

^a TORONTO GENERAL HOSPITAL   (Canada)

^b HÔPITAL TENON   (France)

^c SORBONNE UNIVERSITÉ   (France)

Author keywords

Cytology; Endobronchial ultrasound; Lung cancer; Rapid on site evaluation; Surgical biopsy; Transbronchial needle aspiration

Indexed keywords

ANTIBODY LABELING; ASPIRATION CYTOLOGY; CANCER ADJUVANT THERAPY; CANCER STAGING; CELL DENSITY; CELL LOSS; CELL SUSPENSION; CONSCIOUS SEDATION; CYTOLOGY; CYTOPATHOLOGY; DIAGNOSTIC VALUE; DIFFERENTIAL DIAGNOSIS; ENDOBRONCHIAL ULTRASONOGRAPHY; ENDOBRONCHIAL ULTRASOUND GUIDED TRANSBRONCHIAL NEEDLE ASPIRATION; FALSE POSITIVE RESULT; FLUORESCENCE IN SITU HYBRIDIZATION; GRANULOMATOSIS; HODGKIN DISEASE; HUMAN; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; LARGE CELL LYMPHOMA; LIQUID CULTURE; LOCAL ANESTHESIA; LUNG CANCER; LYMPH NODE METASTASIS; LYMPHADENOPATHY; META ANALYSIS (TOPIC); NODULAR SCLEROSIS HODGKIN LYMPHOMA; PHENOTYPE; PREDICTIVE VALUE; SHORT SURVEY; SYSTEMATIC REVIEW (TOPIC); TRANSBRONCHIAL ASPIRATION;

ANESTHESIA, LOCAL; BIOPSY, FINE-NEEDLE; BRONCHI; BRONCHOSCOPES; BRONCHOSCOPY; CARCINOMA, NON-SMALL-CELL LUNG; CENTRIFUGATION; CONSCIOUS SEDATION; ENDOSONOGRAPHY; EQUIPMENT DESIGN; GRANULOMA; HUMANS; LUNG DISEASES; LUNG NEOPLASMS; LYMPH NODES; LYMPHATIC METASTASIS; LYMPHOMA; MEDIASTINUM; MICROTOMY; NEOPLASM STAGING; PARAFFIN EMBEDDING; SPECIMEN HANDLING; STAINING AND LABELING; TIME FACTORS; ULTRASONOGRAPHY, INTERVENTIONAL;

EID: 84870997022     PISSN: 02426498     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.annpat.2012.09.212     Document Type: Short Survey

References (75)

1. Detterbeck F.C., Jantz M.A., Wallace M., Vansteenkiste J., Silvestri G.A. Invasive mediastinal staging of lung cancer: ACCP evidence-based clinical practice guidelines (2nd edition). Chest 2007, 132:202S-220S.
2. Yasufuku K., Chiyo M., Sekine Y., Chhajed P.N., Shibuya K., Iizasa T., et al. Real-time endobronchial ultrasound-guided transbronchial needle aspiration of mediastinal and hilar lymph nodes. Chest 2004, 126:122-128.
3. Yasufuku K., Chiyo M., Koh E., Moriya Y., Iyoda A., Sekine Y., et al. Endobronchial ultrasound guided transbronchial needle aspiration for staging of lung cancer. Lung Cancer 2005, 50:347-354.
4. Yasufuku K., Nakajima T., Motoori K., Sekine Y., Shibuya K., Hiroshima K., et al. Comparison of endobronchial ultrasound, positron emission tomography, and CT for lymph node staging of lung cancer. Chest 2006, 130:710-718.
5. Gu P., Zhao Y.Z., Jiang L.Y., Zhang W., Xin Y., Han B.H. Endobronchial ultrasound-guided transbronchial needle aspiration for staging of lung cancer: a systematic review and meta-analysis. European Journal of Cancer 2009, 45:1389-1396.
6. Adams K., Shah P.L., Edmonds L., Lim E. Test performance of endobronchial ultrasound and transbronchial needle aspiration biopsy for mediastinal staging in patients with lung cancer: systematic review and meta-analysis. Thorax 2009, 64:757-762.
7. Varela-Lema L., Fernandez-Villar A., Ruano-Ravina A. Effectiveness and safety of endobronchial ultrasound-transbronchial needle aspiration: a systematic review. European Respiratory Journal 2009, 33:1156-1164.
8. Steinfort D.P., Conron M., Tsui A., Pasricha S.R., Renwick W.E., Antippa P., et al. Endobronchial ultrasound-guided transbronchial needle aspiration for the evaluation of suspected lymphoma. J Thorac Oncol 2010, 5:804-809.
9. Ko H.M., da Cunha Santos G., Darling G., Pierre A., Yasufuku K., Boerner S.L., et al. Diagnosis and subclassification of lymphomas and non-neoplastic lesions involving mediastinal lymph nodes using endobronchial ultrasound-guided transbronchial needle aspiration. Diagnostic Cytopathology 2011, 10.1002/dc.21741.
10. Wong M., Yasufuku K., Nakajima T., Herth F.J., Sekine Y., Shibuya K., et al. Endobronchial ultrasound: new insight for the diagnosis of sarcoidosis. European Respiratory Journal 2007, 29:1182-1186.
11. Nakajima T., Yasufuku K., Kurosu K., Takiguchi Y., Fujiwara T., Chiyo M., et al. The role of EBUS-TBNA for the diagnosis of sarcoidosis--comparisons with other bronchoscopic diagnostic modalities. Respiratory Medicine 2009, 103:1796-1800.
12. Tremblay A., Stather D.R., Maceachern P., Khalil M., Field S.K. A randomized controlled trial of standard vs endobronchial ultrasonography-guided transbronchial needle aspiration in patients with suspected sarcoidosis. Chest 2009, 136:340-346.
13. Kennedy M.P., Jimenez C.A., Bruzzi J.F., Mhatre A.D., Lei X., Giles F.J., et al. Endobronchial ultrasound-guided transbronchial needle aspiration in the diagnosis of lymphoma. Thorax 2008, 63:360-365.
14. Rusch V.W., Asamura H., Watanabe H., Giroux D.J., Rami-Porta R., Goldstraw P. The IASLC lung cancer staging project: a proposal for a new international lymph node map in the forthcoming seventh edition of the TNM classification for lung cancer. J Thorac Oncol 2009, 4:568-577.
15. Fujiwara T., Yasufuku K., Nakajima T., Chiyo M., Yoshida S., Suzuki M., et al. The utility of sonographic features during endobronchial ultrasound-guided transbronchial needle aspiration for lymph node staging in patients with lung cancer: a standard endobronchial ultrasound image classification system. Chest 2010, 138:641-647.
16. Cameron S.E., Andrade R.S., Pambuccian S.E. Endobronchial ultrasound-guided transbronchial needle aspiration cytology: a state of the art review. Cytopathology 2010, 21:6-26.
17. Fleury-Feith J., Bernaudin J.F. Cytologic examination in thoracic oncology. Revue des Maladies Respiratoires 2011, 28:254-265.
18. Kocjan G., Chandra A., Cross P., Denton K., Giles T., Herbert A., et al. BSCC Code of Practice--fine needle aspiration cytology. Cytopathology 2009, 20:283-296.
19. Skov B.G., Baandrup U., Jakobsen G.K., Kiss K., Krasnik M., Rossen K., et al. Cytopathologic diagnoses of fine-needle aspirations from endoscopic ultrasound of the mediastinum: reproducibility of the diagnoses and representativeness of aspirates from lymph nodes. Cancer 2007, 111:234-241.
20. Rakha E.A., Naik V., Chaudry Z., Baldwin D., Soomro I.N. Cytological assessment of conventional transbronchial fine needle aspiration of lymph nodes. Cytopathology 2010, 21:27-34.
21. Wallace W.A.H., Monaghan H.M., Salter D.M., Gibbons M.A., Skwarski K.M. Endobronchial ultrasound-guided fine-needle aspiration and liquid-based thin-layer cytology. Journal of Clinical Pathology 2007, 60:388-391.
22. Delattre C., Fournier C., Bouchindhomme B., Renaud F., Escande F., Ramon P., et al. Endoscopic ultrasound guided transbronchial fine needle aspiration: a French Department of Pathology's 4-year experience. Journal of Clinical Pathology 2011, 64:1117-1122.
23. Natu S., Hoffman J., Siddiqui M., Hobday C., Shrimankar J., Harrison R. The role of endobronchial ultrasound guided transbronchial needle aspiration cytology in the investigation of mediastinal lymphadenopathy and masses, the North Tees experience. Journal of Clinical Pathology 2010, 63:445-451.
24. Slater S. Guidance for non-gynaecological cytology--a platform for the future. Cytopathology 2009, 20:279-280.
25. Kim S., Owens C.L. Analysis of ThinPrep cytology in establishing the diagnosis of small cell carcinoma of lung. Cancer 2009, 117:51-56.
26. Nigro K., Tynski Z., Wasman J., Abdul-Karim F., Wang N. Comparison of cell block preparation methods for nongynecologic ThinPrep specimens. Diagnostic Cytopathology 2007, 35:640-643.
27. Fetsch P.A., Abati A. Immunocytochemistry in effusion cytology: a contemporary review. Cancer 2001, 93:293-308.
28. Loukeris K., Vazquez M.F., Sica G., Wagner P., Yankelevitz D.F., Henschke C.I., et al. Cytological cell blocks: predictors of squamous cell carcinoma and adenocarcinoma subtypes. Diagnostic Cytopathology 2012, 40:380-387.
29. Nakajima T., Yasufuku K., Nakagawara A., Kimura H., Yoshino I. Multi-gene mutation analysis of metastatic lymph nodes in non-small cell lung cancer diagnosed by EBUS-TBNA. Chest 2011, 140:1319-1324.
30. Fleury-Feith J., Gounant V., Lacave R., Cadranel J., Bernaudin J.F. Multigene mutation analysis on cytologic samples. Chest 2011, 140:1664-1665.
31. Gounant V., Fleury-Feith J. How do we do it? Another optimal methodology for endobronchial ultrasound sample handling. J Thorac Oncol 2011, 6:972-973.
32. Gounant V., Ninane V., Janson X., Colombat M., Wislez M., Grunenwald D., et al. Release of metal particles from needles used for transbronchial needle aspiration. Chest 2011, 139:138-143.
33. Ladd A.C., O'Sullivan-Mejia E., Lea T., Perry J., Dumur C.I., Dragoescu E., et al. Preservation of fine-needle aspiration specimens for future use in RNA-based molecular testing. Cancer Cytopathol 2011, 119:102-110.
34. Nakajima T., Yasufuku K. How I do it--optimal methodology for multidirectional analysis of endobronchial ultrasound-guided transbronchial needle aspiration samples. J Thorac Oncol 2011, 6:203-206.
35. Diacon A.H., Schuurmans M.M., Theron J., Louw M., Wright C.A., Brundyn K., et al. Utility of rapid on-site evaluation of transbronchial needle aspirates. Respiration 2005, 72:182-188.
36. Nayak A., Sugrue C., Koenig S., Wasserman P.G., Hoda S., Morgenstern N.J. Endobronchial ultrasound-guided transbronchial needle aspirate (EBUS-TBNA): a proposal for on-site adequacy criteria. Diagnostic Cytopathology 2012, 40:128-137.
37. Alsharif M., Andrade R.S., Groth S.S., Stelow E.B., Pambuccian S.E. Endobronchial ultrasound-guided transbronchial fine-needle aspiration: the University of Minnesota experience, with emphasis on usefulness, adequacy assessment, and diagnostic difficulties. American Journal of Clinical Pathology 2008, 130:434-443.
38. Feller-Kopman D., Yung R.C., Burroughs F., Li Q.K. Cytology of endobronchial ultrasound-guided transbronchial needle aspiration: a retrospective study with histology correlation. Cancer 2009, 117:482-490.
39. Gasparini S. It is time for this 'ROSE' to flower. Respiration 2005, 72:129-131.
40. Layfield L.J., Bentz J.S., G E.V. Immediate on-site interpretation of fine-needle aspiration smears. A cost and compensation analysis. Cancer 2001, 93:319-322.
41. Micames C.G., Mc Crory D.C., Pavey D.A., Jowell P.S., Gress F.G. Endoscopic ultrasound-guided fine-needle aspiration for non-small cell lung cancer staging: a systematic review and metaanalysis. Chest 2007, 131:539-548.
42. Al-Abbadi M.A., Bloom L.I., Fatheree L.A., Haack L.A., Minkowitz G., Wilbur D.C., et al. Adequate reimbursement is crucial to support cost-effective rapid on-site cytopathology evaluations. Cytojournal 2010, 7:22.
43. Chandra S., Nehra M., Agarwal D., Mohan A. Diagnostic accuracy of endobronchial ultrasound guided transbronchial needle aspiration in mediastinal lymphadenopathy: a systematic review and meta-analysis. Respiratory Care 2012, 57:384-391.
44. Griffin A.C., Schwartz L.E., Baloch Z.W. Utility of on-site evaluation of endobronchial ultrasound-guided transbronchial needle aspiration specimens. Cytojournal 2011, 8:20.
45. Savoy A.D., Raimondo M., Woodward T.A., Noh K., Pungpapong S., Jones A.D., et al. Can endosonographers evaluate on-site cytologic adequacy. A comparison with cytotechnologists. Gastrointestinal Endoscopy 2007, 65:953-957.
46. Herbert A., Santis G. EBUS-TBNA: an opportunity for clinicians, cytopathologists and patients to gain from multidisciplinary collaboration. Cytopathology 2010, 21:3-5.
47. Casal R.F., Staerkel G.A., Ost D., Almeida F.A., Uzbeck M.H., Eapen G.A., et al. Randomized clinical trial of endobronchial ultrasound needle biopsy with and without aspiration. Chest 2011, 10.1378/CHEST.11-0692.
48. Sanz-Santos J., Andreo F., Castella E., Llatjos M., de Castro P.L., Astudillo J., et al. Representativeness of nodal sampling with endobronchial ultrasonography in non-small-cell lung cancer staging. Ultrasound in Medicine and Biology 2011, 38:62-68.
49. Memoli J.S., El-Bayoumi E., Pastis N.J., Tanner N.T., Gomez M., Huggins J.T., et al. Using endobronchial ultrasound features to predict lymph node metastasis in patients with lung cancer. Chest 2011, 140:1550-1556.
50. Herth F.J.A.J., Eberhardt R., Yasufuku K., Ernst A., Krasnik M., Rintoul R.C. Endobronchial ultrasound with transbronchial needle aspiration for restaging the mediastinum in lung cancer. Journal of Clinical Oncology 2008, 26:3346-3350.
51. Tournoy K.G., Rintoul R.C., van Meerbeeck J.P., Carroll N.R., Praet M., Buttery R.C., et al. EBUS-TBNA for the diagnosis of central parenchymal lung lesions not visible at routine bronchoscopy. Lung Cancer 2009, 63:45-49.
52. Pao W., Hutchinson K.E. Chipping away at the lung cancer genome. Nature Medicine 2012, 18:349-351.
53. Travis W.D., Brambilla E., Noguchi M., Nicholson A.G., Geisinger K.R., Yatabe Y., et al. International association for the study of lung cancer/american thoracic society/european respiratory society international multidisciplinary classification of lung adenocarcinoma. J Thorac Oncol 2011, 6:244-285.
54. Nizzoli R., Tiseo M., Gelsomino F., Bartolotti M., Majori M., Ferrari L., et al. Accuracy of fine needle aspiration cytology in the pathological typing of non-small cell lung cancer. J Thorac Oncol 2011, 6:489-493.
55. Sigel C.S., Moreira A.L., Travis W.D., Zakowski M.F., Thornton R.H., Riely G.J., et al. Subtyping of non-small cell lung carcinoma: a comparison of small biopsy and cytology specimens. J Thorac Oncol 2011, 6:1849-1856.
56. Cornfield D., Liu Z., Gorczyca W., Weisberger J. The potential role of flow cytometry in the diagnosis of small cell carcinoma. Archives of Pathology and Laboratory Medicine 2003, 127:461-464.
57. Farinola M.A., Weir E.G., Ali S.Z. CD56 expression of neuroendocrine neoplasms on immunophenotyping by flow cytometry: a novel diagnostic approach to fine-needle aspiration biopsy. Cancer 2003, 99:240-246.
58. Nicholson A.G., Gonzalez D., Shah P., Pynegar M.J., Deshmukh M., Rice A., et al. Refining the diagnosis and EGFR status of non-small cell lung carcinoma in biopsy and cytologic material, using a panel of mucin staining, TTF-1, cytokeratin 5/6, and P63, and EGFR mutation analysis. J Thorac Oncol 2010, 5:436-441.
59. Pusztaszeri M., Soccal M.P., Mach N., Pache J.C., Mc Kee T. Cytopathological diagnosis of non small cell lung cancer. Recent advances including rapid on site evaluation, novel endoscopic techniques and molecular tests. J Pulm Respir Med 2012, 10.4172/2161-105X.S5-002.
60. Santis G., Angell R., Nickless G., Quinn A., Herbert A., Cane P., et al. Screening for EGFR and KRAS mutations in endobronchial ultrasound derived transbronchial needle aspirates in non-small cell lung cancer using COLD-PCR. PLoS One 2011, 6:e25191.
61. Rekhtman N., Brandt S.M., Sigel C.S., Friedlander M.A., Riely G.J., Travis W.D., et al. Suitability of thoracic cytology for new therapeutic paradigms in non-small cell lung carcinoma: high accuracy of tumor subtyping and feasibility of EGFR and KRAS molecular testing. J Thorac Oncol 2011, 6:451-458.
62. Navani N., Nankivell M., Woolhouse I., Harrison R.N., Munavvar M., Oltmanns U., et al. Endobronchial ultrasound-guided transbronchial needle aspiration for the diagnosis of intrathoracic lymphadenopathy in patients with extrathoracic malignancy: a multicenter study. J Thorac Oncol 2011, 6:1505-1509.
63. Park J., Jang S.J., Park Y.S., Oh Y.M., Shim T.S., Kim W.S., et al. Endobronchial ultrasound-guided transbronchial needle biopsy for diagnosis of mediastinal lymphadenopathy in patients with extrathoracic malignancy. Journal of Korean Medical Science 2011, 26:274-278.
64. Tournoy K.G., Govaerts E., Malfait T., Dooms C. Endobronchial ultrasound-guided transbronchial needle biopsy for M1 staging of extrathoracic malignancies. Annals of Oncology 2011, 22:127-131.
65. Tournoy K.G., Bolly A., Aerts J.G., Pierard P., De Pauw R., Leduc D., et al. The value of endoscopic ultrasound after bronchoscopy to diagnose thoracic sarcoidosis. European Respiratory Journal 2010, 35:1329-1335.
66. Garwood S., Judson M.A., Silvestri G., Hoda R., Fraig M., Doelken P. Endobronchial ultrasound for the diagnosis of pulmonary sarcoidosis. Chest 2007, 132:1298-1304.
67. Hassan T., McLaughlin A.M., O'Connell F., Gibbons N., Nicholson S., Keane J. EBUS-TBNA performs well in the diagnosis of isolated thoracic tuberculous lymphadenopathy. American Journal of Respiratory and Critical Care Medicine 2011, 183:136-137.
68. Marshall C.B., Jacob B., Patel S., Sneige N., Jimenez C.A., Morice R.C., et al. The utility of endobronchial ultrasound-guided transbronchial needle aspiration biopsy in the diagnosis of mediastinal lymphoproliferative disorders. Cancer Cytopathol 2011, 119:118-126.
69. Yasufuku K., Nakajima T., Fujiwara T., Yoshino I., Keshavjee S. Utility of endobronchial ultrasound-guided transbronchial needle aspiration in the diagnosis of mediastinal masses of unknown etiology. Annals of Thoracic Surgery 2011, 91:831-836.
70. Kalikaki A., Koutsopoulos A., Trypaki M., Souglakos J., Stathopoulos E., Georgoulias V., et al. Comparison of EGFR and K-RAS gene status between primary tumours and corresponding metastases in NSCLC. British Journal of Cancer 2008, 99:923-929.
71. Park S., Holmes-Tisch A.J., Cho E.Y., Shim Y.M., Kim J., Kim H.S., et al. Discordance of molecular biomarkers associated with epidermal growth factor receptor pathway between primary tumors and lymph node metastasis in non-small cell lung cancer. J Thorac Oncol 2009, 4:809-815.
72. Schmid K., Oehl N., Wrba F., Pirker R., Pirker C., Filipits M. EGFR/KRAS/BRAF mutations in primary lung adenocarcinomas and corresponding locoregional lymph node metastases. Clinical Cancer Research 2009, 15:4554-4560.
73. da Cunha Santos G., Saieg M.A., Geddie W., Leighl N. EGFR gene status in cytological samples of nonsmall cell lung carcinoma: controversies and opportunities. Cancer Cytopathol 2010, 119:80-91.
74. Marchetti A., Felicioni L., Buttitta F. Assessing EGFR mutations. New England Journal of Medicine 2006, 354:526-528. [author reply -8].
75. Savic S., Tapia C., Grilli B., Rufle A., Bihl M.P., de Vito Barascud A., et al. Comprehensive epidermal growth factor receptor gene analysis from cytological specimens of non-small-cell lung cancers. British Journal of Cancer 2008, 98:154-160.