Preservation of fine-needle aspiration specimens for future use in RNA-based molecular testing

Cancer Cytopathology

Volumn 119, Issue 2, 2011, Pages 103-110

  Ladd, Amy C ^a   O'Sullivan Mejia, Emerald ^a   Lea, Tasha ^a   Perry, Jessica ^a   Dumur, Catherine I ^a   Garrett, Carleton T ^a   Powers, Celeste N ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

Author keywords

Biorepository; Cryopreservation; Fine needle aspiration; FNA; Gene expression; Molecular morphology; RNA; Tissue bank

Indexed keywords

RNA;

ARTICLE; CELL STRUCTURE; CRYOPRESERVATION; FINE NEEDLE ASPIRATION BIOPSY; HUMAN; HUMAN CELL; HUMAN TISSUE; MATERIALS HANDLING; MICROMORPHOLOGY; PRIORITY JOURNAL; RNA ANALYSIS; RNA DEGRADATION; RNA EXTRACTION; STRUCTURE ANALYSIS; CYTOLOGY; GENETICS; HEALTH CARE FACILITY; METABOLISM; METHODOLOGY; NEEDLE BIOPSY; NEOPLASM; PATHOLOGY; PRESERVATION; QUALITY CONTROL; REPRODUCIBILITY; RNA STABILITY; STANDARD; TIME;

BIOPSY, FINE-NEEDLE; CYTOLOGICAL TECHNIQUES; HUMANS; NEOPLASMS; PRESERVATION, BIOLOGICAL; QUALITY CONTROL; REPRODUCIBILITY OF RESULTS; RNA STABILITY; RNA, NEOPLASM; TIME FACTORS; TISSUE BANKS;

EID: 79960917070     PISSN: None     EISSN: 19346638     Source Type: Journal    
DOI: 10.1002/cncy.20130     Document Type: Article

References (18)

1. Simione FP. Cryopreservation Manual. Nalge Nunc International Corp; 1998. http://www.nalgenelabware.com/techdata/Technical/cryo.pdf
2. Quintans CJ, Donaldson MJ, Bertolino MV, Pasqualini RS. Birth of 2 babies using oocytes that were cryopreserved in a choline-based freezing medium. Hum Reprod. 2002; 17:3149-3152.
3. Dumur CI, Sana S, Ladd AC, et al. Assessing the impact of tissue devitalization time on genome-wide gene expression analysis in ovarian tumor samples. Diagn Mol Pathol. 2008; 17:200-206.
4. Cunningham D, Humblet Y, Siena S, et al. Cetuximab monotherapy and cetuximab plus irinotecan in irinotecanrefractory metastatic colorectal cancer. N Engl J Med. 2004; 351:337-345.
5. Pegram MD, Lipton A, Hayes DF, et al. Phase II study of receptor-enhanced chemosensitivity using recombinant humanized anti-p185HER2/neu monoclonal antibody plus cisplatin in patients with HER2/neu-overexpressing metastatic breast cancer refractory to chemotherapy treatment. J Clin Oncol. 1998;16:2659-2671.
6. Druker BJ, Talpaz M, Resta DJ, et al. Efficacy and safety of a specific inhibitor of the BCR-ABL tyrosine kinase in chronic myeloid leukemia. N Engl J Med. 2001;344:1031-1037.
7. Lauwers GY, Black-Schaffer S, Salto-Tellez M. Molecular pathology in contemporary diagnostic pathology laboratory: An opinion for the active role of surgical pathologists. Am J Surg Pathol. 2010;34:115-117.
8. Krishnamurthy S. Applications of molecular techniques to fine-needle aspiration biopsy. Cancer. 2007;111:106-122.
9. Schmitt FC, Longatto-Filho A, Valent A, Vielh P. Molecular techniques in cytopathology practice. J Clin Pathol. 2008;61:258-267.
10. Zhang S, Abreo F, Lowery-Nordberg M, Veillon DM, Cotelingam JD. The role of fluorescence in situ hybridization and polymerase chain reaction in the diagnosis and classification of lymphoproliferative disorders on fine-needle aspiration. Cancer Cytopathol. 2010;118:105-112.
11. Shih JY, Gow CH, Yu CJ, et al. Epidermal growth factor receptor mutations in needle biopsy/aspiration samples predict response to gefitinib therapy and survival of patients with advanced nonsmall cell lung cancer. Int J Cancer. 2006;118:963-969.
12. Ohori NP, Nikiforova MN, Schoedel KE, et al. Contribution of molecular testing to thyroid fine-needle aspiration cytology of ''follicular lesion of undetermined significance/atypia of undetermined significance''. Cancer Cytopathol. 2010;118:17-23.
13. National Cancer Institute. Best Practices for Biospecimen Resources [PDF online]. Bethesda, Maryland; 2007. http://biospecimens.cancer.gov/bestpractices/ Accessed June 21, 2010.
14. Becker C, Hammerle-Fickinger A, Riedmaier I, Pfaffl MW. mRNA and microRNA quality control for RT-qPCR analysis. Methods. 2010;50:237-243.
15. Dumur CI, Nasim S, Best AM, et al. Evaluation of qualitycontrol criteria for microarray gene expression analysis. Clin Chem. 2004;50:1994-2002.
16. Jung M, Schaefer A, Steiner I, et al. Robust microRNA stability in degraded RNA preparations from human tissue and cell samples. Clin Chem. 2010;56:998-1006.
17. Fleige S, Pfaffl MW. RNA integrity and the effect on the real-Time qRT-PCR performance. Mol Aspects Med. 2006; 27:126-139.
18. Gazziero A, Guzzardo V, Aldighieri E, Fassina A. Morphological quality and nucleic acid preservation in cytopathology. J Clin Pathol. 2009;62:429-434.