High-throughput SISCAPA quantitation of peptides from human plasma digests by ultrafast, liquid chromatography-free mass spectrometry

Journal of Proteome Research

Volumn 11, Issue 12, 2012, Pages 5642-5649

  Razavi, Morteza ^a   Frick, Lauren E ^b   Lamarr, William A ^b   Pope, Matthew E ^c   Miller, Christine A ^d   Anderson, N Leigh ^c,e   Pearson, Terry W ^a,c  

^a UNIVERSITY OF VICTORIA   (Canada)

^b Agilent Technologies Inc   (United States)

^c SISCAPA Assay Technologies Inc   (United States)

^d AGILENT TECHNOLOGIES   (United States)

^e HOLLAND LABORATORY   (United States)

Author keywords

antipeptide antibodies; biomarkers; mass spectrometry; multiple reaction monitoring; RapidFire; SISCAPA; SPE MS MS; validation

Indexed keywords

BIOLOGICAL MARKER; LIPOPOLYSACCHARIDE BINDING PROTEIN; MESOTHELIN; MONOCLONAL ANTIBODY; PEPTIDE;

ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DIGESTION; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNOADSORBENT; ISOTOPE LABELING; LIMIT OF QUANTITATION; LIQUID CHROMATOGRAPHY; MAGNETIC BEAD IMMUNOADSORBENT; MASS SPECTROMETRY; MULTIPLE REACTION MONITORING; PLASMA; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BLOOD LEVEL; PROTEIN TARGETING; PROTEOMICS; QUANTITATIVE ANALYSIS; REACTION ANALYSIS; REACTION TIME; SISCAPA METHOD; SOLID PHASE EXTRACTION; TANDEM MASS SPECTROMETRY;

ACUTE-PHASE PROTEINS; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; BIOLOGICAL MARKERS; BLOOD PROTEINS; CARRIER PROTEINS; CHROMATOGRAPHY, LIQUID; GPI-LINKED PROTEINS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MASS SPECTROMETRY; MEMBRANE GLYCOPROTEINS; PEPTIDES; PROTEOMICS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SOFTWARE; TIME FACTORS;

EID: 84870923756     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr300652v     Document Type: Article

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