Multilocus sequence typing (MLST) analysis of 104 Clostridium difficile strains isolated from China

Epidemiology and Infection

Volumn 141, Issue 1, 2013, Pages 195-199

  Yan, Q ^a   Zhang, J ^b   Chen, C ^a   Zhou, H ^a   Du, P ^a   Cui, Z ^a   Cen, R ^a   Liu, L ^b   Li, W ^a   Cao, B ^a   Lu, J ^a   Cheng, Y ^a  

^a National Institute for Communicable Disease Control and Prevention   (China)

^b Shandong Centre for Disease Control and Prevention   (China)

Author keywords

Clostridium difficile; gene polymorphism; MLST

Indexed keywords

ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATION; CHINA; CLOSTRIDIUM DIFFICILE; EPIDEMIC; GENETIC POLYMORPHISM; GENETIC VARIABILITY; GENOTYPE; GEOGRAPHIC ORIGIN; MULTILOCUS SEQUENCE TYPING; NONHUMAN; PHYLOGENY;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; CHILD, PRESCHOOL; CHINA; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; CLUSTER ANALYSIS; FEMALE; HUMANS; INFANT; MALE; MIDDLE AGED; MOLECULAR EPIDEMIOLOGY; MULTILOCUS SEQUENCE TYPING; PHYLOGENY; YOUNG ADULT;

EID: 84870912303     PISSN: 09502688     EISSN: 14694409     Source Type: Journal    
DOI: 10.1017/S0950268812000453     Document Type: Article

References (14)

1. Bartlett JG, et al. Antibiotic-associated pseudomembranous colitis due to toxin producing clostridia. New England Journal of Medicine 1978; 298: 531-534. (Pubitemid 8295586)
2. Spencer RC. Clinical impact and associated costs of Clostridium difficile-associated disease. Journal of Antimicrobial Chemotherapy 1998; 41: 5-12. (Pubitemid 28230294)
3. McEllistrem MC, et al. A hospital outbreak of Clostridium difficile disease associated with isolates carrying binary toxin genes. Clinical Infectious Diseases 2005; 40: 265-272. (Pubitemid 40110672)
4. Bauer MP, et al. Clostridium difficile infection in Europe: A hospital-based survey. Lancet. 2011; 377: 63-73.
5. Cheng VC, et al. Clostridium difficile isolates with increased sporulation: Emergence of PCR ribotype 002 in Hong Kong. European Journal of Clinical Microbiology & Infectious Diseases 2011; 30: 1371-1381.
6. Huang H, et al. Clostridium difficile infections in a Shanghai hospital: Antimicrobial resistance, toxin profiles and ribotypes. International Journal of Antimicrobial Agents 2009; 33: 339-342.
7. Bishara J, et al. Molecular epidemiology of Clostridium difficile in a tertiary medical center in Israel: Emergence of the polymerase chain reaction ribotype O27. Israel Medical Association Journal 2011; 13: 338-341.
8. Maiden MC. Multilocus sequence typing of bacteria. Annual Review of Microbiology 2006; 60: 561-588. (Pubitemid 44627961)
9. Griffiths D, et al. Multilocus sequence typing of Clostridium difficile. Journal of Clinical Microbiology 2010; 48: 770-778.
10. Kato H, et al. Identification of toxin A-negative, toxin B-positive Clostridium difficile by PCR. Journal of Clinical Microbiology 1998; 36: 2178-2182. (Pubitemid 28324061)
11. Leme'e L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (toxin A), and tcdB (toxin B) genes for toxigenic culture of Clostridium difficile. Journal of Clinical Microbiology 2004; 42: 5710-5714.
12. Leme'e L, Pons JL. Multilocus sequence typing for Clostridium difficile. Methods in Molecular Biology 2010; 646: 77-90.
13. Dingle KE, et al. Clinical Clostridium difficile: Clonality and pathogenicity locus diversity. PLoS One 2011; 6: E19993.
14. Cheng Y, et al. Toxin A-negative, Toxin B-positive Clostridium difficile infection diagnosed by PCR. Infection Control and Hospital Epidemiology 2011; 32: 520-522.