A high-throughput assay for enzymatic polyester hydrolysis activity by fluorimetric detection

Biotechnology Journal

Volumn 7, Issue 12, 2012, Pages 1517-1521

  Wei, Ren ^a   Oeser, Thorsten ^a   Billig, Susan ^a   Zimmermann, Wolfgang ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

Biocatalysis; Enzymes; Fluorescence; High throughput; Screening

Indexed keywords

96-WELL; BIOCATALYSIS; BUFFER CONCENTRATIONS; DEGRADATION PRODUCTS; FLUORIMETRIC ASSAYS; FLUORIMETRIC DETECTION; FREE HYDROXYL RADICALS; HIGH-THROUGHPUT; HIGH-THROUGHPUT ASSAYS; HIGH-THROUGHPUT SCREENING; HPLC METHOD; HYDROLYSIS ACTIVITY; MICROPLATES; NUMBER OF SAMPLES; PET FILMS; PH VALUE; SYNTHETIC POLYMERS; TEREPHTHALATE; TEREPHTHALIC ACIDS; THERMOBIFIDA FUSCA;

DEGRADATION; ENZYMATIC HYDROLYSIS; ENZYMES; FLUORESCENCE; HYDROLASES; POLYETHYLENE TEREPHTHALATES; SCREENING;

ESTERS;

HYDROLASE; NANOPARTICLE; POLYETHYLENE TEREPHTHALATE;

ARTICLE; AUTOOXIDATION; BIOCATALYST; FLUOROMETRY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; HYDROLYSIS; HYDROXYLATION; NONHUMAN; PH; PRIORITY JOURNAL; REACTION TIME; THERMOBIFIDA FUSCA;

ACTINOMYCETALES; BACTERIAL PROTEINS; BIOREACTORS; BIOTECHNOLOGY; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROLASES; PHTHALIC ACIDS; POLYETHYLENE TEREPHTHALATES; SPECTROMETRY, FLUORESCENCE;

THERMOBIFIDA FUSCA;

EID: 84870703309     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201200119     Document Type: Article

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