A fluorometric method to quantify protein glutathionylation using glutathione derivatization with 2,3-naphthalenedicarboxaldehyde

Analytical Biochemistry

Volumn 433, Issue 2, 2013, Pages 132-136

  Menon, Deepthi ^a   Board, Philip G ^a  

^a Australian National University   (Australia)

Author keywords

Detection of glutathionylation with 2,3 naphthalenedicarboxaldehyde; Glutathionylation; Quantification of glutathionylation

Indexed keywords

ACETONE; CELL CULTURE; HISTOLOGY; MAMMALS; MUSCLE; PEPTIDES; PHOSPHORUS COMPOUNDS; SULFUR COMPOUNDS;

DETERMINATION OF PROTEINS; FLUORESCENT PRODUCTS; FLUOROMETRIC METHOD; GLUTATHIONYLATION; LYMPHOBLASTOID CELLS; RAPID MEASUREMENT; S-NITROSOGLUTATHIONE; TRIS(2 CARBOXYETHYL)PHOSPHINE;

CELLS;

2,3 NAPHTHALENEDICARBOXALDEHYDE; ACETONE; DOXORUBICIN; GLUTATHIONE; GLUTATHIONE DERIVATIVE; N ETHYLMALEIMIDE; S NITROSOGLUTATHIONE; TRIS(2 CARBOXYETHYL)PHOSPHINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN; CHEMICAL REACTION; CONTROLLED STUDY; FLUOROMETRY; GLUTATHIONYLATION; HEART; IMMUNOBLOTTING; KIDNEY; LIMIT OF DETECTION; LIVER; LUNG; LYMPHOBLASTOID CELL; MALE; MOUSE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN DETERMINATION; QUANTITATIVE ASSAY; SPLEEN; TESTIS; TISSUE HOMOGENATE; WESTERN BLOTTING;

MUS;

EID: 84870653940     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.10.009     Document Type: Article

References (31)

1. I. Dalle-Donne, R. Rossi, G. Colombo, D. Giustarini, and A. Milzani Protein S-glutathionylation: a regulatory device from bacteria to humans Trends Biochem. Sci. 34 2009 85 96
2. M.D. Shelton, P.B. Chock, and J.J. Mieyal Glutaredoxin: role in reversible protein S-glutathionylation and regulation of redox signal transduction and protein translocation Antioxid. Redox Signal. 7 2005 348 366
3. J.J. Mieyal, M.M. Gallogly, S. Qanungo, E.A. Sabens, and M.D. Shelton Molecular mechanisms and clinical implications of reversible protein S-glutathionylation Antioxid. Redox Signal. 10 2008 1941 1988
4. M. Lindahl, A. Mata-Cabana, and T. Kieselbach The disulfide proteome and other reactive cysteine proteomes: analysis and functional significance Antioxid. Redox Signal. 14 2011 2581 2642
5. K. Lei, D.M. Townsend, and K.D. Tew Protein cysteine sulfinic acid reductase (sulfiredoxin) as a regulator of cell proliferation and drug response Oncogene 27 2008 4877 4887
6. A.J. Cooper, J.T. Pinto, and P.S. Callery Reversible and irreversible protein glutathionylation: biological and clinical aspects Expert Opin. Drug Metab. Toxicol. 7 2011 891 910
7. D.M. Sullivan, N.B. Wehr, M.M. Fergusson, R.L. Levine, and T. Finkel Identification of oxidant-sensitive proteins: TNF-alpha induces protein glutathiolation Biochemistry 39 2000 11121 11128
8. C. Lind, R. Gerdes, Y. Hamnell, I. Schuppe-Koistinen, H.B. von Lowenhielm, A. Holmgren, and I.A. Cotgreave Identification of S-glutathionylated cellular proteins during oxidative stress and constitutive metabolism by affinity purification and proteomic analysis Arch. Biochem. Biophys. 406 2002 229 240
9. X.H. Gao, M. Bedhomme, D. Veyel, M. Zaffagnini, and S.D. Lemaire Methods for analysis of protein glutathionylation and their application to photosynthetic organisms Mol. Plant 2 2009 218 235
10. M. Fratelli, H. Demol, M. Puype, S. Casagrande, P. Villa, I. Eberini, J. Vandekerckhove, E. Gianazza, and P. Ghezzi Identification of proteins undergoing glutathionylation in oxidatively stressed hepatocytes and hepatoma cells Proteomics 3 2003 1154 1161
11. E.T. Chouchani, A.M. James, I.M. Fearnley, K.S. Lilley, and M.P. Murphy Proteomic approaches to the characterization of protein thiol modification Curr. Opin. Chem. Biol. 15 2011 120 128
12. G. Cheng, Y. Ikeda, Y. Iuchi, and J. Fujii Detection of S-glutathionylated proteins by glutathione S-transferase overlay Arch. Biochem. Biophys. 435 2005 42 49
13. P.S. Tsou, V. Addanki, J.A. Haas, N.A. Page, and H.L. Fung Role of glutaredoxin-mediated protein S-glutathionylation in cellular nitroglycerin tolerance J. Pharmacol. Exp. Ther. 329 2009 649 656
14. E. Camera, and M. Picardo Analytical methods to investigate glutathione and related compounds in biological and pathological processes J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 781 2002 181 206
15. D.M. Townsend, Y. Manevich, L. He, S. Hutchens, C.J. Pazoles, and K.D. Tew Novel role for glutathione S-transferase pi: regulator of protein S-glutathionylation following oxidative and nitrosative stress J. Biol. Chem. 284 2009 436 445
16. J.L. Gilge, M. Fisher, and Y.C. Chai The effect of oxidant and the non-oxidant alteration of cellular thiol concentration on the formation of protein mixed-disulfides in HEK 293 cells PLoS One 3 2008 e4015
17. C. Zhang, C. Rodriguez, M.L. Circu, T.Y. Aw, and J. Feng S-glutathionyl quantification in the attomole range using glutaredoxin-3-catalyzed cysteine derivatization and capillary gel electrophoresis with laser-induced fluorescence detection Anal. Bioanal. Chem. 401 2011 2165 2175
18. S.W. Aesif, V. Anathy, I. Kuipers, A.S. Guala, J.N. Reiss, Y.S. Ho, and Y.M. Janssen-Heininger Ablation of glutaredoxin-1 attenuates lipopolysaccharide-induced lung inflammation and alveolar macrophage activation Am. J. Respir. Cell Mol. Biol. 44 2011 491 499
19. O. Orwar, H.A. Fishman, N.E. Ziv, R.H. Scheller, and R.N. Zare Use of 2,3-naphthalenedicarboxaldehyde derivatization for single-cell analysis of glutathione by capillary electrophoresis and histochemical localization by fluorescence microscopy Anal. Chem. 67 1995 4261 4268
20. C.C. White, H. Viernes, C.M. Krejsa, D. Botta, and T.J. Kavanagh Fluorescence-based microtiter plate assay for glutamate-cysteine ligase activity Anal. Biochem. 318 2003 175 180
21. P.G. Board, and I. Hutchinson A fluorescence-based microtiter plate assay for γ-glutamylcyclotransferase Anal. Biochem. 420 2011 177 181
22. A.D. Luca, N. Moroni, A. Serafino, A. Primavera, A. Pastore, J.Z. Perdersen, R. Petruzzelli, M.G. Frarrace, P. Pierimarchi, G. Moroni, G. Federici, P.S. Vallebona, and M.L. Bello Treatment of doxorubicin resistant MCF7/Dx cells with nitric oxide causes histone glutathionylation and reversal of drug resistance Biochem. J. 440 2011 175 183
23. J.-W. Park Reaction of S-nitrosoglutathione with sulfhydryl groups in protein Biochem. Biophys. Res. Commun. 152 1988 916 920
24. O.H. Lowry, N.J. Rosenbrough, A.L. Farr, and R.J. Randall Protein measurement with the Folin-Phenol reagent J. Biol. Chem. 193 1951 265 275
25. H. Towbin, T. Staehelin, and J. Gordon Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Proc. Natl. Acad. Sci. USA 76 1979 4350 4354
26. S.S. Tate, and A. Meister Serine-borate complex as a transition-state inhibitor of gamma-glutamyl transpeptidase Proc. Natl. Acad. Sci. USA 75 1978 4806 4809
27. A. Meister, and M.E. Anderson Glutathione Annu. Rev. Biochem. 52 1983 711 760
28. B.G. Hill, and A. Bhatnagar Protein S-glutathiolation: redox-sensitive regulation of protein function J. Mol. Cell. Cardiol. 52 2012 559 567
29. A.J. Oakley, T. Yamada, D. Liu, M. Coggan, A.G. Clark, and P.G. Board The identification and structural characterization of C7orf24 as gamma-glutamyl cyclotransferase: an essential enzyme in the gamma-glutamyl cycle J. Biol. Chem. 283 2008 22031 22042
30. R. Hakanson, and A.L. Ronnberg Improved fluorometric assay of histamine: condensation with O-phthalaldehyde at -20° Anal. Biochem. 60 1974 560 567
31. I. Kuipers, K.R. Bracke, G.G. Brusselle, S.W. Aesif, R. Krijgsman, I.C. Arts, E.F. Wouters, and N.L. Reynaert Altered cigarette smoke-induced lung inflammation due to ablation of grx1 PLoS One 7 2012 e38984