The effect of oxidant and the non-oxidant alteration cellular thiol concentration on the formation of protein mixed-disulfides in HEK 293 cells

PLoS ONE

Volumn 3, Issue 12, 2008, Pages

  Gilge, Jasen Lee ^a   Fisher, Michael ^a   Chai, Yuh Cherng ^a  

^a JOHN CARROLL UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BUTHIONINE SULFOXIMINE; CYSTINE; DIAMIDE; GLUTATHIONE; HYDROGEN PEROXIDE; METHIONINE; ANTIOXIDANT; DISULFIDE; OXIDIZING AGENT; PROTEIN DISULFIDE ISOMERASE; THIOL DERIVATIVE;

ANTIOXIDANT RESPONSIVE ELEMENT; ARTICLE; CELL STRAIN HEK293; CELLULAR DISTRIBUTION; CELLULAR STRESS RESPONSE; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; OXIDATIVE STRESS; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN INDUCTION; REGULATORY MECHANISM; SIGNAL TRANSDUCTION; CELL LINE; CHEMISTRY; CYTOLOGY; KIDNEY; METABOLISM; PHYSIOLOGY; PRENATAL DEVELOPMENT; PROTEIN PROCESSING;

ANTIOXIDANTS; CELL LINE; DISULFIDES; GLUTATHIONE; HUMANS; KIDNEY; OXIDANTS; OXIDATIVE STRESS; PROTEIN DISULFIDE-ISOMERASES; PROTEIN PROCESSING, POST-TRANSLATIONAL; SULFHYDRYL COMPOUNDS;

EID: 58149193255     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0004015     Document Type: Article

References (28)

1. Sies H (1991) Oxidative Stress. II. Oxidants and Antioxidants. London: Academic Press.
2. Oktyabrsky ON, Smirnova GV (2007) Redox Regulation of Cellular Functions. Biochemistry 72: 132-145.
3. Reed DJ (1990) Glutathione: toxicological implications. Annu Rev Pharmacol Toxicol 30: 603-631.
4. Ishii T, Yanagawa T (2007) Stress-induced peroxiredoxins. Subcell Biochem 44: Available: http://www.ncbi.nlm.nih.gov/pubmed/ 18084904?ordinalpos=3&itool= EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Accessed 2008 Aug 19.
5. Ghezzi P, Bonetto V (2003) Redox proteomics: identification of oxidatively modified proteins. Proteomics 3: 1145-53.
6. Ghezzi P (2005) Oxidoreduction of protein thiols in redox regulation. Biochemical Society Transactions 33: 1378-1381.
7. Dalle-Donne I, Rossi R, Giustarini D, Colombo R, Milzani A (2007) S-glutathionylation in protein redox regulation. Free Radical Biology & Medicine 43: 883-898.
8. Yu J, Zhang NN, Yin PD, Cui Px, Zhou CZ (2008) Glutathionylation-triggered conformational changes of glutaredoxin Grx1 from the yeast Saccharomyces cerevisiae. Proteins 72: Available: http://www.ncbi.nlm.nih.gov/sites/entrez. Accessed 2008 Aug 31.
9. Thomas JA, Mallis RJ (2001) Aging and oxidation of reactive protein sulfhydryls. Experimental Gerontology 36: 1519-1526.
10. Ziegler DM (1985) Role of reversible oxidation-reduction of enzyme thiols-disulfides in metabolic regulation. Ann Rev Biochem 54: 305-329.
11. Di Simplicio P, Frosali S, Priora R, Summa D, Di Simplicio FC, et al. (2005) Biochemical and biological aspects of protein thiolation in cells and plasma. Antioxidants & Redox Signaling 7: 951-963.
12. Anselmo AN, Cobb MH (2004) Protein kinase function and glutathionylation. Biochem J 381: e1-e2.
13. Giustarini D, Rossi R, Milzani A, Colombo R, Dalle-Donne I (2004) S-glutathionylation: from redox regulation of protein functions to human diseases. J Cell Mol Med 8: 201-212.
14. Wang J, Boja ES, Tan W, Tekle E, Fales Hm, et al. (2001) Reversible glutathionylation regulates actin polymerization in A431 cells. J Biol Chem 276(51): 47763-6.
15. Shackelford RE, Heinloth AN, Heard SC, Paules RS (2005) Cellular and molecular targets of protein s-glutathionylation. Antioxidants & Redox Signaling 7: 940-950.
16. Townsend DM (2007) S-glutathionylation indicator of cell stress and regulator of the unfolded protein response. Molecular Interventions 7: 313-324.
17. Shelton MD, Chock PB, Mieyal JJ (2005) Glutaredoxin: Role in reversible protein s-glutathionylation and regulation of redox signal transduction and protein translocation. Antioxidants and Redox Signaling 7: 348-366.
18. Gallogly MM, Mieyal JJ (2007) Mechanisms of reversible protein glutathionylation in redox signaling and oxidant stress. Current Opinion in Pharmacology 7: 381-391.
19. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
20. Anderson ME (1998) Glutathione: an overview of biosynthesis and modulation. Chem Biol Interact; Available: http://www.ncbi.nlm.nih.gov/ pubmed/9679538?ordinalpos=6&itool= EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum. Accessed 2008 Aug 13.
21. Kalef E, Walfish PG, Gitler C (1993) Anal Biochem 212: 325-334.
22. Reed DJ, Babson JR, Beatty PW, Brodie AE, Ellis WW, et al. (1980) High-performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulfide, and related thiols and disulfides. Anal Biochem 106: 55-62.
23. Ying J, Clavreul N, Sethuraman M, Takeshi A, Cohen RA (2007) thiol oxidation in signaling and response to stress: detection and quantification of physiological and pathophysiological thiol modicifications. Free Radical Biology & Medicine 43: 1099-1108.
24. Wang J, Boja ES, Tan W, Tekle E, Fales HM, et al. (2001) Reversible glutathionylation regulates actin polymerization in A431 cells. J Biol Chem 276: Available: http://www.jbc.org/cgi/gca?allch= &SEARCHID=1&VOLUME276&F IRSTPAGE=47763&FIRSTINDEX=0&hits=10&RESULTFORMAT=1&gca= jbc%3B276%2F51%2F47763. Accessed 2008 Aug 31.
25. Reinhardt C, Bruhl ML, Manukyan D, Grahl L, Lorenz M, et al. (2008) Protein disulfide isomerase acts as an injury response signal that enhances fibrin generation via tissue factor activation. J Clin Invest 118: Available: http://www.jci.org/articles/view/32376. Acessed 2008 Aug 31.
26. Leonberg AK, Chai YC (2007) The functional role of cysteine residues for c-Abl kinase activity. Mol Cell Biochem 304: 207-212.
27. Kosower NS, Kosower EM (1995) Diamide: an oxidant probe for thiols. Methods Enzymol 251: Available: http://www.ncbi.nlm.nih.gov/pubmed/ 7651192?ordinalpos=3&itool= EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum. Accessed 2008 Aug 13.
28. Chai YC, Ashraf SS, Rokutan K, Johnston RB, Thomas JA (1994) S-thiolation of individual human neutrophil proteins including actin by stimulation of the respiratory burst: evidence against a role for glutathione disulfide. Arch Biochem Biophys 310: 264-272.