Monitoring local synaptic activity with astrocytic patch pipettes

Nature Protocols

Volumn 7, Issue 12, 2012, Pages 2171-2179

  Henneberger, Christian ^a,b   Rusakov, Dmitri A ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ASTROCYTE; BRAIN SLICE; CLINICAL PROTOCOL; CONTROLLED STUDY; ELECTRIC RESISTANCE; EXPERIMENTAL DESIGN; EXTRACELLULAR SPACE; ION TRANSPORT; NERVOUS SYSTEM ELECTROPHYSIOLOGY; NONHUMAN; PATCH CLAMP; PIPETTE; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; SYNAPTIC POTENTIAL; WHOLE CELL;

ASTROCYTES; ELECTROPHYSIOLOGY; PATCH-CLAMP TECHNIQUES; SYNAPSES; SYNAPTIC TRANSMISSION;

EID: 84870624562     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2012.140     Document Type: Article

References (33)

1. Haydon, P.G. Glia: Listening and talking to the synapse. Nat. Rev. Neurosci. 2, 185-193 (2001 (Pubitemid 33673371)
2. Volterra, A. & Meldolesi, J. Astrocytes, from brain glue to communication elements: The revolution continues. Nat. Rev. Neurosci. 6, 626-640 (2005 (Pubitemid 41059337)
3. Nedergaard, M., Rodriguez, J.J. & Verkhratsky, A. Glial calcium and diseases of the nervous system. Cell Calcium 47, 140-149 (2010
4. Perea, G. & Araque, A. Glia modulates synaptic transmission. Brain Res. Rev. 63, 93-102 (2010
5. Han, J. et al. Acute cannabinoids impair working memory through astroglial CB1 receptor modulation of hippocampal LTD. Cell 148, 1039-1050 (2012
6. Navarrete, M. et al. Astrocytes mediate in vivo cholinergic-induced synaptic plasticity. PLoS Biol. 10, e1001259 (2012
7. Takata, N. et al. Astrocyte calcium signaling transforms cholinergic modulation to cortical plasticity in vivo. J. Neurosci. 31, 18155-18165 (2011
8. Hamilton, N.B. & Attwell, D. Do astrocytes really exocytose neurotransmitters? Nat. Rev. Neurosci. 11, 227-238 (2010
9. Kang, J., Jiang, L., Goldman, S.A. & Nedergaard, M. Astrocyte-mediated potentiation of inhibitory synaptic transmission. Nat. Neurosci. 1, 683-692 (1998 (Pubitemid 128406379)
10. Pascual, O. et al. Astrocytic purinergic signaling coordinates synaptic networks. Science 310, 113-116 (2005 (Pubitemid 41434548)
11. Serrano, A., Haddjeri, N., Lacaille, J.C. & Robitaille, R. Gabaergic network activation of glial cells underlies hippocampal heterosynaptic depression. J. Neurosci. 26, 5370-5382 (2006 (Pubitemid 44318389)
12. Navarrete, M. & Araque, A. Endocannabinoids mediate neuron-astrocyte communication. Neuron 57, 883-893 (2008 (Pubitemid 351400634)
13. Navarrete, M. & Araque, A. Endocannabinoids potentiate synaptic transmission through stimulation of astrocytes. Neuron 68, 113-126 (2010
14. Agulhon, C. et al. What is the role of astrocyte calcium in neurophysiology? Neuron 59, 932-946 (2008
15. Perea, G., Navarrete, M. & Araque, A. Tripartite synapses: Astrocytes process and control synaptic information. Trends Neurosci. 32, 421-431 (2009
16. Panatier, A. et al. Astrocytes are endogenous regulators of basal transmission at central synapses. Cell 146, 785-798 (2011
17. Bergles, D.E. & Jahr, C.E. Synaptic activation of glutamate transporters in hippocampal astrocytes. Neuron 19, 1297-1308 (1997 (Pubitemid 28030548)
18. Bergles, D.E. & Jahr, C.E. Glial contribution to glutamate uptake at Schaffer collateral-commissural synapses in the hippocampus. J. Neurosci. 18, 7709-7716 (1998 (Pubitemid 28441012)
19. Diamond, J.S., Bergles, D.E. & Jahr, C.E. Glutamate release monitored with astrocyte transporter currents during LTP. Neuron 21, 425-433 (1998 (Pubitemid 28399702)
20. Luscher, C., Malenka, R.C. & Nicoll, R.A. Monitoring glutamate release during LTP with glial transporter currents. Neuron 21, 435-441 (1998 (Pubitemid 28399703)
21. Scimemi, A., Fine, A., Kullmann, D.M. & Rusakov, D.A. NR2B-containing receptors mediate cross talk among hippocampal synapses. J. Neurosci. 24, 4767-4777 (2004 (Pubitemid 38668201)
22. Steinhäuser, C., Berger, T., Frotscher, M. & Kettenmann, H. Heterogeneity in the membrane current pattern of identified glial cells in the hippocampal slice. Eur. J. Neurosci. 4, 472-484 (1992
23. Henneberger, C., Papouin, T., Oliet, S.H. & Rusakov, D.A. Long-term potentiation depends on release of d-serine from astrocytes. Nature 463, 232-236 (2010
24. Ge, W.P. & Duan, S.M. Persistent enhancement of neuron-glia signaling mediated by increased extracellular K+ accompanying long-term synaptic potentiation. J. Neurophysiol. 97, 2564-2569 (2007
25. Tsukada, S., Iino, M., Takayasu, Y., Shimamoto, K. & Ozawa, S. Effects of a novel glutamate transporter blocker, (2S, 3S)-3-[3-[4- (trifluoromethyl)benzoylamino]benzyloxy]aspartate (TFB-TBOA), on activities of hippocampal neurons. Neuropharmacology 48, 479-491 (2005 (Pubitemid 40332406)
26. Molleman, A. Patch Clamping: An Introductory Guide to Patch Clamp Electrophysiology (Wiley & Sons, 2002
27. Lein, P.J., Barnhart, C.D. & Pessah, I.N. Acute hippocampal slice preparation and hippocampal slice cultures. Methods Mol. Biol. 758, 115-134 (2011
28. Anderson, P., Bliss, T.V. & Skrede, K.K. Lamellar organization of hippocampal pathways. Exp. Brain Res. 13, 222-238 (1971
29. Teyler, T.J. Brain slice preparation: Hippocampus. Brain Res. Bull. 5, 391-403 (1980 (Pubitemid 11236918)
30. Schwartzkroin, P.A. Characteristics of CA1 neurons recorded intracellularly in the hippocampal in vitro slice preparation. Brain Res. 85, 423-436 (1975
31. Scott, R., Ruiz, A., Henneberger, C., Kullmann, D.M. & Rusakov, D.A. Analog modulation of mossy fiber transmission is uncoupled from changes in presynaptic Ca2+. J. Neurosci. 28, 7765-7773 (2008
32. Nimmerjahn, A., Kirchhoff, F., Kerr, J.N. & Helmchen, F. Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat. Methods 1, 31-37 (2004
33. Kang, K. & Song, M.R. Diverse FGF receptor signaling controls astrocyte specification and proliferation. Biochem. Biophys. Res. Comm. 395, 324-329 (2010).