Multichannel bipotentiostat integrated with a microfluidic platform for electrochemical real-time monitoring of cell cultures

IEEE Transactions on Biomedical Circuits and Systems

Volumn 6, Issue 5, 2012, Pages 498-507

  Vergani, Marco ^a   Carminati, Marco ^a   Ferrari, Giorgio ^a   Landini, Ettore ^b   Caviglia, Claudia ^c   Heiskanen, Arto ^c   Comminges, Clément ^d   Zor, Kinga ^c   Sabourin, David ^c   Dufva, Martin ^c   Dimaki, Maria ^c   Raiteri, Roberto ^b   Wollenberger, Ulla ^d   Emneus, Jenny ^c   Sampietro, Marco ^a  

^a POLITECNICO DI MILANO   (Italy)

^b UNIVERSITY OF GENOA   (Italy)

^c TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^d UNIVERSITY OF POTSDAM   (Germany)

Author keywords

Electrochemical measurements; impedance spectroscopy; microfluidics; multichannel potentiostat; stem cell monitoring

Indexed keywords

AMPEROMETRY; ANALYTES; ARRAYS OF MICROELECTRODES; BUFFER SOLUTIONS; CELL MONITORING; CONTINUOUS TRACKING; CURRENT RESOLUTION; DATA ACQUISITION SOFTWARES; ELECTROCHEMICAL DETECTION SYSTEMS; ELECTROCHEMICAL MEASUREMENTS; ELECTRONIC BOARDS; EXPERIMENTAL CHARACTERIZATION; IMPEDANCE SPECTROSCOPY; INPUT CAPACITANCE; MICRO FLUIDIC SYSTEM; MICROFLUIDIC CELL CULTURE; MICROFLUIDIC PLATFORMS; MICROFLUIDIC PUMPS; MULTI-CHANNEL; MULTIPARAMETERS; ON CURRENTS; PC12 CELLS; POTENTIOSTATS; REAL TIME MONITORING;

CELL CULTURE; CYCLIC VOLTAMMETRY; MICROELECTRODES; SPECTROSCOPY; STEM CELLS; VOLTAGE REGULATORS;

MICROFLUIDICS;

DOPAMINE;

ANIMAL; ARTICLE; BIOMEDICAL ENGINEERING; CELL PROLIFERATION; COMPUTER PROGRAM; COMPUTER SYSTEM; CULTURE TECHNIQUE; ELECTROCHEMICAL ANALYSIS; ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; EQUIPMENT; EQUIPMENT DESIGN; GENETIC PROCEDURES; MICROFLUIDIC ANALYSIS; PC12 CELL LINE; POTENTIOMETRY; RAT; SIGNAL PROCESSING; STATISTICS;

ANIMALS; BIOMEDICAL ENGINEERING; BIOSENSING TECHNIQUES; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; COMPUTER SYSTEMS; DIELECTRIC SPECTROSCOPY; DOPAMINE; ELECTROCHEMICAL TECHNIQUES; EQUIPMENT DESIGN; MICROFLUIDIC ANALYTICAL TECHNIQUES; PC12 CELLS; POTENTIOMETRY; RATS; SIGNAL PROCESSING, COMPUTER-ASSISTED; SOFTWARE;

EID: 84870555099     PISSN: 19324545     EISSN: None     Source Type: Journal    
DOI: 10.1109/TBCAS.2012.2187783     Document Type: Article

References (23)

1. F. H. Gage, "Mammalian neural stem cells, " Science, vol. 287, pp. 1433-1438, 2000. (Pubitemid 30127128)
2. O. Lindvall, Z. Kokaia, and A. Martinez-Serrano, "Stem cell therapy for human neurodegenerative disorders-How to make it work, " Nat. Med., vol. 10, pp. S42-S50, 2004. (Pubitemid 38901866)
3. F. J. Sigworth and K. G. Klemic, "Microchip technology in ion-channel research, " IEEE Trans. Nanobiosci., vol. 4, no. 1, pp. 121-127, 2005. (Pubitemid 40384653)
4. P. Weerakoon, E. Culurciello, K. G. Klemic, and F. J. Sigworth, "An integrated patch-clamp potentiostat with electrode compensation, " IEEE Trans. Biomed. Circuits Syst., vol. 3, pp. 117-125, 2009.
5. L. Berdondini, K. Imfeld, A. Maccione, M. Tedesco, S. Neukom, M. Koudelka-Hep, and S. Martinoia, "Active pixel sensor array for high spatio-temporal resolution electrophysiological recordings from single cell to large scale neuronal networks, " Lab Chip, vol. 9, pp. 2644-2651, 2009.
6. R. M. Wightman, "Probing cellular chemistry in biological systems with microelectrodes, " Science, vol. 311, pp. 1570-1574, 2006.
7. I. L. Jones, P. Livi, M. K. Lewandowska, M. Fiscella, B. Roscic, and A. Hierlemann, "The potential of microelectrode arrays and microelectronics for biomedical research and diagnostics, " Anal. Bioanal. Chem., vol. 399, pp. 2313-2329, 2011.
8. L. Li, X. Liu, W. A. Qureshi, and A. J. Mason, "CMOS amperometric instrumentation and packaging for biosensor array applications, " IEEE Trans. Biomed. Circuits Syst., vol. 5, pp. 439-448, Oct. 2011.
9. J. Gottschamel, L. Richter, A. Mak, C. Jungreuthmayer, G. Birnbaumer, M. Milnera, H. Brückl, and P. Ertl, "Development of a disposable microfluidic biochip for multiparameter cell population measurements, " Anal. Chem., vol. 81, pp. 8503-8512, 2009.
10. X. Yue, E. M. Drakakis, M. Lim, A. Radomska, H. Ye, A. Mantalaris, N. Panoskaltsis, and A. Cass, "A real-time multi-channel monitoring system for stem cell culture process, " IEEE Trans. Biomed. Circuits Syst., vol. 2, no. 2, pp. 66-77, Apr. 2008.
11. C. Spégel, A. Heiskanen, L. H. D. Skjolding, and J. Emnéus, "Chip based electroanalytical systems for cell analysis, " Electroanal., vol. 20, pp. 680-702, 2008.
12. A. J. Bard and L. R. Faulkner, Electrochemical Methods: Fundamentals and Application, 2001.
13. C. Spégel, A. Heiskanen, J. Acklid, A. Wolff, R. Taboryski, J. Emnéus, and T. Ruzgas, "On-chip determination of dopamine exocytosis using mercaptopropionic acid modified microelectrodes, " Electroanal., vol. 19, pp. 263-271, 2007.
14. P. O. Bagnaninchi and N. Drummond, "Real-time label-free monitoring of adipose-derived stem cell differentiation with electric cell-substrate impedance sensing, " Proc. Nat. Acad. Sci., vol. 108, pp. 6462-6467, 2011.
15. H. E. Park, D. Kim, H. S. Koh, S. Cho, J.-S. Sung, and J. Y. Kim, "Realtime monitoring of neural differentiation of human mesenchymal stem cells by electric cell-substrate impedance sensing, " J. Biomed. Biotech., pp. 485173-485191, 2011.
16. P. Skafte-Pedersen, D. Sabourin, M. Dufva, and D. Snakenborg, "Multi-channel peristaltic pump for microfluidic applications featuring monolithic PDMS inlay, " Lab Chip, vol. 9, pp. 3003-3006, 2009.
17. I. Giaever and C. R. Keese, "Monitoring fibroblast behavior in tissue culture with an applied electric field, " Proc. Nat. Acad. Sci., vol. 81, pp. 3761-3764, 1984. (Pubitemid 14087798)
18. L. Wang, L. Wang, H. Yin, W. Xing, Z. Yu, M. Guo, and J. Cheng, "Real-time, label-free monitoring of the cell cycle with a cellular impedance sensing chip, " Biosens. Bioelectron., vol. 25, pp. 990-995, 2010.
19. M. Paeschke, U. Wollenberger, C. Köhler, T. Lisec, U. Schnakenberg, and R. Hintsche, "Properties of interdigital electrode arrays with different geometries, " Anal. Chim. Acta, vol. 305, pp. 126-136, 1995.
20. R. M. Wightman and D. O. Wipf, "High-speed cyclic voltammetry, " Accounts Chem. Res., vol. 23, pp. 64-70, 1990.
21. A. Heiskanen, C. Spégel, J. Tønnesen, Z. Fohlerova, L. Goulart, J. Hansen, M. Kokaia, M. Dufva, and J. Emnéus, "Development of a microfluidic on-line culture system for combined electrochemical and optical real-time detection of cellular processes, " in Proc. μTAS, San Diego, CA, 2008, vol. 2, pp. 1168-1171.
22. K. Zór, M. Vergani, A. Heiskanen, E. Landini, M. Carminati, V. Coman, I. Vedarethinam, P. Skafte-Pedersen, M. Skolimowski, A. M. Serrano, M. Kokaia, T. R. Moreno, A. Ghio, W. E. Svendsen, M. Dimaki, Z. Kerestes, M. Adamovski, U. Wollenberger, D. Sabourin, G. Ferrari, R. Raiteri, M. Sampietro, M. Dufva, and J. Emnéus, "Real-time monitoring of cellular dynamics using a microfluidic cell culture system with integrated electrode array and potentiostat, " in Proc. μTAS, Seattle, WA, 2011, vol. 1, pp. 1532-1535.
23. M. Carminati, G. Ferrari, and M. Sampietro, "Attofarad resolution potentiostat for electrochemical measurements on nanoscale biomolecular interfacial systems, " Rev. Sci. Instrum., vol. 80, pp. 124701:1-124701:10, 2009.