Methods for monitoring gene gun-induced HBV- and HCV-specific immune responses in mouse models

Methods in Molecular Biology

Volumn 940, Issue , 2013, Pages 239-267

  Ahlén, Gustaf ^a   Sällberg, Matti ^a   Frelin, Lars ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Gene gun; Genetic vaccination; HBV; HCV; Hepatitis; Transiently transgenic mice; Tumor challenge model

Indexed keywords

NS3 PROTEIN, HEPATITIS C VIRUS; VIRUS PROTEIN; VIRUS VACCINE;

ANIMAL; ARTICLE; BIOLISTIC TRANSFORMATION; GENETICS; HEPATITIS B VIRUS; HEPATITIS C VIRUS; IMMUNITY; IMMUNOHISTOCHEMISTRY; IMMUNOLOGY; IMMUNOPRECIPITATION; INSTRUMENTATION; LUMINESCENCE; MOUSE; POLYACRYLAMIDE GEL ELECTROPHORESIS; SKIN TUMOR; SPECIES DIFFERENCE; TRANSGENIC MOUSE; WESTERN BLOTTING;

ANIMALS; BIOLISTICS; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEPACIVIRUS; HEPATITIS B VIRUS; IMMUNITY; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; LUMINESCENT MEASUREMENTS; MICE; MICE, TRANSGENIC; SKIN NEOPLASMS; SPECIES SPECIFICITY; VIRAL NONSTRUCTURAL PROTEINS; VIRAL VACCINES;

HEPATITIS B VIRUS; HEPATITIS C VIRUS; MURINAE; MUS; MUS MUSCULUS;

EID: 84870533503     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-110-3_20     Document Type: Article

References (20)

1. Alter MJ (1997) Epidemiology of hepatitis C. Hepatology 26:62S-65S
2. Milich DR (1997) Pathobiology of acute and chronic hepatitis B virus infection: an introduction. J Viral Hepat 4(suppl 2):25-30
3. Zoulim F, Locarnini S (2009) Hepatitis B virus resistance to nucleos(t)ide analogues. Gastroenterology 137:1593-1608
4. Manns MP et al (2001) Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial. Lancet 358:958-965
5. McHutchison JG et al (2009) Telaprevir with peginterferon and ribavirin for chronic HCV genotype 1 infection. N Engl J Med 360:1827-1838
6. Ferrari C et al (1990) Cellular immune response to hepatitis B virus-encoded antigens in acute and chronic hepatitis B virus infection. J Immunol 145:3442-3449
7. Bowen DG, Walker CM (2005) Adaptive immune responses in acute and chronic hepatitis C virus infection. Nature 436:946-952
8. Yang NS et al (1990) In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment. Proc Natl Acad Sci USA 87:9568-9572
9. Tang DC, DeVit M, Johnston SA (1992) Genetic immunization is a simple method for eliciting an immune response. Nature 356:152-154
10. Chisari FV (1996) Hepatitis B virus transgenic mice: models of viral immunobiology and pathogenesis. Curr Top Microbiol Immunol 206:149-173
11. Pasquinelli C et al (1997) Hepatitis C virus core and E2 protein expression in transgenic mice. Hepatology 25:719-727
12. Frelin L et al (2006) The hepatitis C virus and immune evasion: non-structural 3/4A transgenic mice are resistant to lethal tumour necrosis factor alpha mediated liver disease. Gut 55:1475-1483
13. Kriegs M et al (2009) The hepatitis C virus non-structural NS5A protein impairs both the innate and adaptive hepatic immune response in vivo. J Biol Chem 284:28343-28351
14. Lerat H et al (2002) Steatosis and liver cancer in transgenic mice expressing the structural and nonstructural proteins of hepatitis C virus. Gastroenterology 122:352-365
15. Ahlen G et al (2005) In vivo clearance of hepatitis C virus nonstructural 3/4A-expressing hepatocytes by DNA vaccine-primed cytotoxic T lymphocytes. J Infect Dis 192:2112-2116
16. Frelin L et al (2003) Low dose and gene gun immunization with a hepatitis C virus nonstructural (NS) 3 DNA-based vaccine containing NS4A inhibit NS3/4A-expressing tumors in vivo. Gene Ther 10:686-699
17. Frelin L et al (2004) Codon optimization and mRNA ampli fication effectively enhances the immunogenicity of the hepatitis C virus nonstructural 3/4A gene. Gene Ther 11: 522-533
18. Nystrom J et al (2010) Improving on the ability of endogenous hepatitis B core antigen to prime cytotoxic T lymphocytes. J Infect Dis 201:1867-1879
19. Roy MJ et al (2000) Induction of antigenspeci fic CD8+ T cells, T helper cells, and protective levels of antibody in humans by particle-mediated administration of a hepatitis B virus DNA vaccine. Vaccine 19:764-778
20. Frelin L et al (2009) A mechanism to explain the selection of the hepatitis e antigen-negative mutant during chronic hepatitis B virus infection. J Virol 83:1379-1392