An innovative flow cytometric approach for small-size platelet microparticles: Influence of calcium

Thrombosis and Haemostasis

Volumn 108, Issue 2, 2012, Pages 373-383

  Montoro Garcia, Silvia ^a,b   Shantsila, Eduard ^a   Orenes Piňero, Esteban ^b   Lozano, Maria Luisa ^c   Lip, Gregory Y H ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

^b HOSPITAL UNIVERSITARIO VIRGEN DE LA ARRIXACA   (Spain)

^c HOSPITAL UNIVERSITARIO MORALES MESEGUER   (Spain)

Author keywords

Annexin V; Calcium; Flow cytometry; Phosphatidylserine; Small size microparticles; Strep tavidin

Indexed keywords

BETA3 INTEGRIN; CALCIUM; CALCIUM CHLORIDE; CD63 ANTIGEN; GLYCOPROTEIN IB ALPHA; LIPOCORTIN 5; PADGEM PROTEIN; PHOSPHATIDYLSERINE; THROMBIN; THROMBOCYTE CONCENTRATE;

ANTIBODY LABELING; ARTICLE; CELL ISOLATION; CELL STIMULATION; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; NORMAL HUMAN; PLATELET MICROPARTICLE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; THROMBOCYTE FREE PLASMA; THROMBOCYTE POOR PLASMA; THROMBOCYTE RICH PLASMA;

ADULT; ANNEXIN A5; BIOLOGICAL MARKERS; BIOTINIDASE; BLOOD PLATELETS; CALCIUM; CELL-DERIVED MICROPARTICLES; DOSE-RESPONSE RELATIONSHIP, DRUG; FLOW CYTOMETRY; HEPARIN; HUMANS; PHOSPHATIDYLSERINES; PLATELET GLYCOPROTEIN GPIB-IX COMPLEX; PROTEIN BINDING; STREPTAVIDIN;

EID: 84870361882     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1160/TH12-02-0120     Document Type: Article

References (32)

1. Shcherbina A, Remold-O'Donnell E. Role of caspase in a subset of human platelet activation responses. Blood 1999; 93:4222-4231.
2. Owens AP, 3rd, Mackman N. Microparticles in haemostasis and thrombosis. Circ Res 2011; 108: 1284-1297.
3. Tsimerman G, Roguin A, Bachar A, et al. Involvement of microparticles in diabetic vascular complications. Thromb Haemost 2011; 106: 310-321.
4. Shantsila E, Kamphuisen PW, Lip GY. Circulating microparticles in cardiovascular disease: implications for atherogenesis and atherothrombosis. J Thromb Haemost 2010; 8: 2358-2368.
5. Trappenburg MC, van Schilfgaarde M, Marchetti M, et al. Elevated procoagulant microparticles expressing endothelial and platelet markers in essential thrombo-cythemia. Haematologica 2009; 94: 911-918.
6. Jimenez JJ, Jy W, Mauro LM, et al. Endothelial microparticles released in throm-botic thrombocytopenic purpura express von Willebrand factor and markers of endothelial activation. Br J Haematol 2003; 123: 896-902.
7. Horstman LL, Jy W, Jimenez JJ, et al. Endothelial microparticles as markers of endothelial dysfunction. Front Biosci 2004; 9: 1118-1135.
8. Jayachandran M, Litwiller RD, Owen WG, et al. Characterization of blood borne microparticles as markers of premature coronary calcification in newly meno-pausal women. Am J Physiol Heart Circ Physiol 2008; 295: H931-H938.
9. Lacroix R, Robert S, Poncelet P, et al. Standardization of platelet-derived micro-particle enumeration by flow cytometry with calibrated beads: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop. J Thromb Haemost 2010; 8: 2571-2574.
10. Robert S, Poncelet P, Lacroix R, et al. Standardization of platelet-derived micro-particle counting using calibrated beads and a Cytomics FC500 routine flow cyto-meter: a first step towards multicenter studies? J Thromb Haemost 2009; 7: 190-197.
11. Orozco AF, Lewis DE. Flow cytometric analysis of circulating microparticles in plasma. CytometryA 2010; 77: 502-514.
12. Dragovic RA, Gardiner C, Brooks AS, et al. Sizing and phenotyping of cellular vesicles using Nanoparticle Tracking Analysis. Nanomedicine 2011; 7: 780-788.
13. Yuana Y, Oosterkamp TH, Bahatyrova S, et al. Atomic force microscopy: a novel approach to the detection of nanosized blood microparticles. J Thromb Haemost 2010;8:315-323.
14. Ayers L, Kohler M, Harrison P, et al. Measurement of circulating cell-derived microparticles by flow cytometry: sources of variability within the assay. Thromb Res 2011; 127:370-377.
15. Chandler WL, Yeung W, Tait JF. A new microparticle size calibration standard for use in measuring smaller microparticles using a new flow cytometer. J Thromb Haemost 2011; 9: 1216-1224.
16. Lacroix R, Robert S, Poncelet P, et al. Overcoming limitations of microparticle measurement by flow cytometry. Semin Thromb Hemost 2010; 36: 807-818.
17. van Tits LJ, van Heerde WL, Landburg PP, et al. Plasma annexin A5 and microparticle phosphatidylserine levels are elevated in sickle cell disease and increase further during painful crisis. Biochem Biophys Res Commun 2009; 390:161-164.
18. Connor DE, Exner T, Ma DD, et al. The majority of circulating platelet-derived microparticles fail to bind annexin V, lackphospholipid-dependent procoagulant activity and demonstrate greater expression of glycoprotein lb. Thromb Haemost 2010;103:1044-1052.
19. Fathi F, Altiraihi T, Mowla SJ, et al. Formation of embryoid bodies from mouse embryonic stem cells cultured on silicon-coated surfaces. Cytotechnology 2009; 59: 11-16.
20. Plaza EM, Lozano ML, Sanchez Guiu I, et al. Evaluation of platelet function during extended storage in additive solution, prepared in a new container that allows manual buffy-coat platelet pooling and leucoreduction in the same system. Blood Transfus 2012; epub ahead of print.
21. Bogusiewicz A, Mock NI, Mock DM. Instability of the biotin-protein bond in human plasma. Anal Biochem 2004; 327: 156-1561.
22. Yuana Y, Bertina RM, Osanto S. Pre-analytical and analytical issues in the analysis of blood microparticles. Thromb Haemost. 2011; 105: 396-408.
23. Shah MD, Bergeron AL, Dong JF, et al. Flow cytometric measurement of microparticles: pitfalls and protocol modifications. Platelets 2008; 19: 365-372.
24. Richter RP, Him JL, Tessier B, et al. On the kinetics of adsorption and two-dimensional self-assembly of annexin A5 on supported lipidbilayers. Biophys J 2005; 89: 3372-3385.
25. Tsai WJ, Chen JC, Wang CT. Changes in both calcium pool size and morphology of human platelets incubated in various concentrations of calcium ion. Calcium-specific bleb formation on platelet-membrane surface. Biochim Biophys Acta 1988; 940:105-120.
26. Lentz BR. Exposure of platelet membrane phosphatidylserine regulates blood coagulation. Prog Lipid Res 2003; 42: 423-438.
27. Robert S, Lacroix R, Poncelet P, et al. High-sensitivity flow cytometry provides access to standardized measurement of small-size microparticles. Arterioscler Thromb Vase Biol 2012; Epub ahead of print.
28. Li CQ, Vindigni A, Sadler JE, et al. Platelet glycoprotein lb alpha binds to throm-bin anion-binding exosite II inducing allosteric changes in the activity of throm-bin. J Biol Chem 2001; 276: 6161-6168.
29. Hourdille P, Heilmann E, Combrie R, et al. Thrombin induces a rapid redistribution of glycoprotein Ib-IX complexes within the membrane systems of activated human platelets. Blood 1990; 76: 1503-1513.
30. Gyôrgy B, Módos K, Pállinger E, et al. Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. Blood 2011; 117: e39-48.
31. Dasgupta SK, Guchhait P, Thiagarajan P. Lactadherin binding and phosphatidylserine expression on cell surface-comparison with annexin A5. Transl Res 2006; 148: 19-25.
32. Shi J, Gilbert GE. Lactadherin inhibits enzyme complexes of blood coagulation by competing for phospholipid-binding sites. Blood 2003; 101: 2628-2636.