Removal of 3-Epi-25-hydroxyvitamin D3 interference by liquid chromatography-tandem mass spectrometry is not required for the measurement of 25-hydroxyvitamin D3 in patients older than 2 years

Clinical Chemistry

Volumn 58, Issue 12, 2012, Pages 1719-1720

  Wright, Michael J P ^a   Halsall, David J ^a   Keevil, Brian G ^b  

^a ADDENBROOKE S HOSPITAL   (United Kingdom)

^b MANCHESTER UNIVERSITY NHS FOUNDATION TRUST   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

3 EPI 25 HYDROXYVITAMIN D3; CALCIFEDIOL; UNCLASSIFIED DRUG;

HUMAN; LETTER; LIMIT OF DETECTION; LIQUID CHROMATOGRAPHY; PROCESS DESIGN; REFERENCE VALUE; SIGNAL NOISE RATIO; SOLID PHASE EXTRACTION; TANDEM MASS SPECTROMETRY; VITAMIN BLOOD LEVEL;

ADULT; ANALYTIC SAMPLE PREPARATION METHODS; CALCIFEDIOL; CHROMATOGRAPHY, LIQUID; HUMANS; INFANT; STEREOISOMERISM; TANDEM MASS SPECTROMETRY;

EID: 84870355210     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2012.191460     Document Type: Letter

References (5)

1. Singh RJ, Taylor RL, Reddy GS, Grebe SK. C3-epimers can account for a significant proportion of total circulating 25-hydroxyvitamin D in infants, complicating accurate measurement and interpretation of vitamin D status. J Clin Endocrinol Metab 2006;91:3055-61.
2. 3 in infants, children, and adults. Clin Chem 2011;57:1:1618-9.
3. Strathmann FG, Sadilkova K, Laha TJ, LeSourdd SE, Bornhorstd JA, Hoofnagle AN, Jack R. 3-epi-25 hydroxyvitamin D concentrations are not correlated with age in a cohort of infants and adults. Clin Chim Acta 2012;413:203-6.
4. 3 is present in adult serum. J Clin Endocrinol Metab 2012;97:163-8.
5. Keevil BG. Does the presence of 3-epi-25OHD 3 affect the routine measurement of vitamin D using liquid chromatography tandem mass spectrometry? Clin Chem Lab Med 2011;50:181-2.