Primary culture of human adipocyte precursor cells: Expansion and differentiation

Methods in Molecular Biology

Volumn 806, Issue , 2012, Pages 215-226

  Skurk, Thomas ^a   Hauner, Hans ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Collagenase digestion; Preadipocyte isolation; Serum free culture; Stromal vascular fraction; Tissue culture

Indexed keywords

ADIPOCYTE; ARTICLE; CELL DIFFERENTIATION; CELL SEPARATION; CYTOLOGY; HUMAN; METABOLISM; METHODOLOGY; PRIMARY CELL CULTURE;

ADIPOCYTES; CELL DIFFERENTIATION; CELL SEPARATION; HUMANS; PRIMARY CELL CULTURE;

EID: 82355165008     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-367-7_15     Document Type: Article

References (17)

1. Wabitsch M., Brenner R.E., Melzner I. et al. (2001) Characterization of a human preadipocyte cell strain with high capacity for adipose differentiation. Int. J. Obes. Relat. Metab. Disord. 25, 8-15. (Pubitemid 32142378)
2. Rodbell M. (1964) Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J. Biol. Chem. 239, 375-380.
3. Ailhaud G. and Hauner H. (1998) Development of white adipose tissue. In: Bray GA, Bouchard C, James WPT, eds. Handbook of Obesity. 1st ed. New York, USA. Marcell Dekker Inc. 359-378.
4. Carey G.B. (1998) Mechanisms regulating adipocyte lipolysis. Adv. Exp. Med. Biol. 441, 157-170. (Pubitemid 28428989)
5. Ramsay T.G. (1996) Fat cells. Endocrinol. Metab. Clin. North Am. 25, 847-870. (Pubitemid 27016935)
6. Trayhurn P., Wood I.S. (2005) Signalling role of adipose tissue: adipokines and inflammation in obesity. Biochem. Soc. Trans. 33, 1078-1081. (Pubitemid 41659117)
7. Skurk T., Herder C., Kräft I., Müller-Scholze S., Hauner H., Kolb H. (2005) Production and release of macrophage migration inhibitory factor from human adipocytes. Endocrinology 146, 1006-1011. (Pubitemid 40289288)
8. Crandall D.L., Growling T.M., Busler D.E., Antrilli T.M. (2000) Release of PAI-1 by human preadipocytes and adipocytes independent of insulin and IGF-1. Biochem. Biophys. Res. Commun. 279, 984-988. (Pubitemid 32042889)
9. Fried S.K., Moustaid-Moussa N. (2001) Culture of adipose tissue and isolated adipocytes. Methods Mol. Biol. 155, 197-212.
10. Trayhurn P., Wang B., Wood I.S. (2008) Hypoxia in adipose tissue: a basis for the dysregulation of tissue function in obesity? Br. J. Nutr. 100, 227-235.
11. Sommer B., Sattler G. (2000) Current concepts of fat graft survival: histology of aspirated adipose tissue and review of the literature. Dermatol. Surg. 26, 1159-1166. (Pubitemid 32064720)
12. Entenmann G., Hauner H. (1996) Relationship between replication and differentiation in cultured human adipocyte precursor cells. Am. J. Physiol. 270, C1011-6.
13. Hauner H., Röhrig K., Petruschke T. (1995) Effects of epidermal growth factor (EGF), platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) on human adipocyte development and function. Eur. J. Clin. Invest. 25, 90-96.
14. Skurk T., Ecklebe S., Hauner H.(2007) A novel technique to propagate primary human preadipocytes without loss of differentiation capacity. Obesity (Silver Spring) 15, 2925-2931. (Pubitemid 351256653)
15. van Harmelen V., Skurk T., Röhrig K., Lee Y.-M., Halbleib M., Aprath-Husmann I., Hauner H. (2003) Effect of BMI and age on adipose tissue cellularity and differentiation capacity in women. Int. J. Obes. Relat. Metab. Disord. 27, 889-895. (Pubitemid 36918609)
16. Björntorp P., Karlsson M., Pertoft H., Pettersson P., Sjöström L., Smith U. (1978) Isolation and characterization of cells from rat adipose tissue developing into adipocytes. J. Lipid Res. 19, 316-324.
17. Hauner H., Entenmann G., Wabitsch M., Gaillard D., Ailhaud G., Negrel R., Pfeiffer E.F. (1989) Promoting effect of glucocorticoids on the differentiation of human adipocyte precursor cells in a chemically defined medium. J. Clin. Invest. 85, 1663-1670. (Pubitemid 19272257)