Automation of genomic DNA isolation from formalin-fixed, paraffin-embedded tissues

Pathology Research and Practice

Volumn 208, Issue 12, 2012, Pages 705-707

  Sam, Soya S ^a   Lebel, Kimberly A ^b   Bissaillon, Cheryl L ^b   Tafe, Laura J ^a   Tsongalis, Gregory J ^a   Lefferts, Joel A ^a  

^a DARTMOUTH HITCHCOCK MEDICAL CENTER   (United States)

^b BAYSTATE HEALTH   (United States)

Author keywords

DNA isolation; FFPE tissue; Qiagen EZ1 Tissue Kit; Real time PCR

Indexed keywords

FORMALDEHYDE; GENOMIC DNA; PARAFFIN;

ARTICLE; AUTOMATION; CONTROLLED STUDY; DIAGNOSTIC KIT; DNA DETERMINATION; DNA EXTRACTION; DNA ISOLATION; GAPDH GENE; GENE AMPLIFICATION; HEAD AND NECK TUMOR; HOUSEKEEPING GENE; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; LUNG TUMOR; MOLECULAR DIAGNOSIS; REAL TIME POLYMERASE CHAIN REACTION; TISSUE SECTION;

AUTOMATION; DNA; DNA MUTATIONAL ANALYSIS; FORMALDEHYDE; GENOME; GENOME, HUMAN; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); HEAD AND NECK NEOPLASMS; HUMANS; LUNG NEOPLASMS; PARAFFIN EMBEDDING; REAGENT KITS, DIAGNOSTIC; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; TIME FACTORS; TISSUE FIXATION;

EID: 84870290441     PISSN: 03440338     EISSN: 16180631     Source Type: Journal    
DOI: 10.1016/j.prp.2012.08.008     Document Type: Article

References (12)

1. Blow N. Tissue preparation: tissue issues. Nature 2007, 448:959-963.
2. Lehmann U., Kreipe H. Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies. Methods 2001, 25:409-418.
3. Gnanapragasam V.J. Unlocking the molecular archive: the emerging use of formalin-fixed paraffin-embedded tissue for biomarker research in urological cancer. BJU Int. 2010, 105:274-278.
4. Ludyga N., Grünwald B., Azimzadeh O., Englert S., Höfler H., Tapio S., Aubele M. Nucleic acids from long-term preserved FFPE tissues are suitable for downstream analyses. Virchows Arch. 2012, 460:131-140.
5. Gilbert M.T., Haselkorn T., Bunce M., Sanchez J.J., Lucas S.B., Jewell L.D., Van Marck E., Worobey M. The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?. PLoS One 2007, 2:e537.
6. Turashvili G., Yang W., McKinney S., Kalloger S., Gale N., Ng Y., Chow K., Bell L., Lorette J., Carrier M., Luk M., Aparicio S., Huntsman D., Yip S. Nucleic acid quantity and quality from paraffin blocks: defining optimal fixation, processing and DNA/RNA extraction techniques. Exp. Mol. Pathol. 2012, 92:33-43.
7. Huang W.Y., Sheehy T.M., Moore L.E., Hsing A.W., Purdue M.P. Simultaneous recovery of DNA and RNA from formalin-fixed paraffin-embedded tissue and application in epidemiologic studies. Cancer Epidemiol. Biomarkers Prev. 2010, 19:973-977.
8. Regan J.F., Furtado M.R., Brevnov M.G., Jordan J.A. A sample extraction method for faster, more sensitive PCR-based detection of pathogens in blood culture. J. Mol. Diagn. 2012, 14:120-129.
9. Okello J.B., Zurek J., Devault A.M., Kuch M., Okwi A.L., Sewankambo N.K., Bimenya G.S., Poinar D., Poinar H.N. Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues. Anal. Biochem. 2010, 400:110-117.
10. Huijsmans C.J., Damen J., van der Linden J.C., Savelkoul P.H., Hermans M.H. Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications. BMC Res. Notes 2010, 3:239.
11. Unlocking your FFPE archive: critical factors for molecular analysis of FFPE samples Qiagen FFPE Brochure, 2010. Available at: http://www.qiagen.com/literature/render.aspx?id=200780.
12. Kerick M., Isau M., Timmermann B., Sültmann H., Herwig R., Krobitsch S., Schaefer G., Verdorfer I., Bartsch G., Klocker H., Lehrach H., Schweiger M.R. Targeted high throughput sequencing in clinical cancer settings: formaldehyde fixed-paraffin embedded (FFPE) tumor tissues, input amount and tumor heterogeneity. BMC Med. Genom. 2011, 4:68.