Disseminated neoplasia in clams Venerupis aurea from Galicia (NW Spain): Histopathology, ultrastructure and ploidy of the neoplastic cells, and comparison of diagnostic procedures

Journal of Invertebrate Pathology

Volumn 112, Issue 1, 2013, Pages 16-19

  Carballal, María J ^a   Iglesias, David ^a   Díaz, Seila ^a   Villalba, Antonio ^a  

^a CENTRO DE INVESTIGACIÓNS MARIÑAS   (Spain)

Author keywords

Cell ploidy; Cell ultrastructure; Diagnostic techniques; Histology; Neoplasia; Venerupis aurea

Indexed keywords

ANIMAL; ARTICLE; BIVALVE; COMPARATIVE STUDY; FLOW CYTOMETRY; PLOIDY; SPAIN; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ANIMALS; BIVALVIA; FLOW CYTOMETRY; MICROSCOPY, ELECTRON, TRANSMISSION; PLOIDIES; SPAIN;

BIVALVIA; MOLLUSCA; VENERUPIS AUREA;

EID: 84870165280     PISSN: 00222011     EISSN: 10960805     Source Type: Journal    
DOI: 10.1016/j.jip.2012.10.003     Document Type: Article

References (18)

1. Barber B.J. Neoplastic diseases of commercially important marine bivalves. Aquat. Living Resour. 2004, 17:449-466.
2. Carballal M.J., López M.C., Azevedo C., Villalba A. Hemolymph cell types of the mussel Mytilus galloprovincialis. Dis. Aquat. Organ. 1997, 29:127-135.
3. Collins C. Studies on a Neoplasm of the Cockle, Cerastoderma edule (Linnaeus) 1998, Zoology and Animal Ecology, National University of Ireland, Cork, pp. 159.
4. Cooper K.R., et al. Accuracy of blood cytological screening techniques for the diagnosis of a possible hematopoietic neoplasm in the bivalve mollusc Mya arenaria. J. Invertebr. Pathol. 1982, 39:281-289.
5. Da Silva P.M., Soudant P., Carballal M.J., Lambert C., Villalba A. Cytometric DNA content analysis of neoplastic cells in haemolymph of the cockle Cerastoderma edule. Dis. Aquat. Organ. 2005, 67:133-139.
6. Delaporte M., Synard S., Pariseau J., Mckenna P., Tremblay R., Davidson J., Berthe F.C.J. Assessment of haemic neoplasia in different soft shell clam Mya arenaria populations from eastern Canada by flow cytometry. J. Invertebr. Pathol. 2008, 98:190-197.
7. Díaz S., Cao A., Villalba A., Carballal M.J. Expression of mutant protein p53 and Hsp70 and Hsp90 chaperons in cockles Cerastoderma edule affected by neoplasia. Dis. Aquat. Organ. 2010, 90:215-222.
8. Elston R.A., Moore J.D., Brooks K.M. Disseminated neoplasia of bivalve molluscs. Aquat. Sci. 1992, 6:405-466.
9. Farley C.A. Selected aspects of neoplastic progression in mollusks. Progressive Stages of Malignant Growth/Development. Part. 4: Comparative Aspects of Tumour Progression 1986, vol. 2:1-14. Springer, Berlin. ninth ed.
10. Fegan, D.F., 2000. Evaluation of diagnostic tests: the epidemiological approach. In: Walker, P., Subasinghe, R.P. (Eds.), DNA-Based Molecular Diagnostic Techniques. Research Needs for Standardization and Validation of the Detection of Aquatic Animal Pathogens and Diseases. FAO Fish. Tech. Pap. vol. 395, pp. 30-37.
11. Le Grand, F., K.E., de Montaudouin, X., Villalba, A., Marty, Y., Soudant, P., 2010. Prevalence, intensity, and aneuploidy patterns of disseminated neoplasia in cockles (Cerastoderma edule) from Archachon Bay: seasonal variation and position in sediment, J. Invertebr. Pathol. 104, 110-118.
12. Lowe D.M., Moore M.N. Cytology and quantitative cytochemistry of a proliferative atypical hemocytic condition in Mytilus edulis (Bivalvia, Mollusca). J. Natl. Cancer Inst. 1978, 60:1455-1459.
13. Moore J.D., Elston R.A., Drum A.S., Wilkinson M.T. Alternate pathogenesis of systemic neoplasia in the bivalve mollusc Mytilus. J. Invertebr. Pathol. 1991, 58:231-243.
14. OIE, 2011. Manual of diagnostic tests for aquatic animals. <>http://www.oie.int/en/international-standard-setting/aquatic-manual/access-online/.
15. Peters E.C. Recent Investigations on the Diseminated Sarcomas of Marine Bivalve Molluscs 1988, vol. 18. American Fisheries Society Special Publication, pp. 74-92.
16. Reno P.W., House M., Illingworth A. Flow cytometric and chromosome analysis of softshell clams, Mya arenaria, with disseminated neoplasia. J. Invertebr. Pathol. 1994, 64:163-172.
17. Smolarz K., Renault T., Soletchnik P., Wolowicz M. Neoplasia detection in Macoma balthica from the Gulf of Gdansk: comparison of flow cytometry, histology and chromosome analysis. Dis. Aquat. Organ. 2005, 65:187-195.
18. Villalba A., Carballal M.J., López C., Cabada A., Corral L., Azevedo C. Branchial rickettsia-like infection associated with clam Venerupis rhomboides mortality. Dis. Aquat. Organ. 1999, 36:53-60.