Day 3 embryo selection by metabolomic profiling of culture medium with near-infrared spectroscopy as an adjunct to morphology: A randomized controlled trial

Human Reproduction

Volumn 27, Issue 8, 2012, Pages 2304-2311

  Vergouw, Carlijn G ^a   Kieslinger, Dorit C ^a   Kostelijk, E Hanna ^a   Botros, Lucy L ^b   Schats, Roel ^a   Hompes, Peter G ^a   Sakkas, Denny ^b   Lambalk, Cornelis B ^a  

^a VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^b Molecular Biometrics Inc   (United States)

Author keywords

Embryo morphology; Embryo selection; Metabolomic profiling; Near infrared spectroscopy; RCT

Indexed keywords

ADULT; ARTICLE; BIRTH RATE; CONTROLLED STUDY; CULTURE MEDIUM; DOUBLE BLIND PROCEDURE; EMBRYO SEGMENTATION; EMBRYO TRANSFER; FEMALE; FERTILIZATION IN VITRO; HUMAN; LIVE BIRTH; MAJOR CLINICAL STUDY; METABOLOMICS; MORPHOLOGY; NEAR INFRARED SPECTROSCOPY; NEWBORN DISEASE; PREGNANCY DISORDER; RANDOMIZED CONTROLLED TRIAL; TWIN PREGNANCY;

EID: 84870037455     PISSN: 02681161     EISSN: 14602350     Source Type: Journal    
DOI: 10.1093/humrep/des175     Document Type: Article

References (22)

1. Ahlstrom A, Wikland M, Rogberg L, Barnett JS, Tucker M, Hardarson T. Cross-validation and predictive value of near-infrared spectroscopy algorithms for day-5 blastocyst transfer. Reprod Biomed Online 2011; doi:10.1016/j.rbmo. 2011.01.009.
2. Filho ES, Noble JA, Wells D. A review on automatic analysis of human embryo microscope images. Open Biomed Eng J 2010;4:170-177.
3. Hardarson T, Ahlstrom A, Rogberg L, Botros L, HillensjöT, Westlander G, Sakkas D, Wikland Ml. Non-invasive metabolomic profiling of day 2 and 5 embryo culture medium: a prospective randomised trial. Hum Reprod 2012;27:89-96.
4. Hollywood K, Brison DR, Goodacre R. Metabolomics: current technologies and future trends. Proteomics 2006;6:4716-4723. (Pubitemid 44375842)
5. Kallen B, Finnstrom O, Lindam A, Nilsson E, Nygren KG, Otterblad OP. Trends in delivery and neonatal outcome after in vitro fertilization in Sweden: data for 25 years. Hum Reprod 2010;25:1026-1034.
6. Kjellberg AT, Carlsson P, Bergh C. Randomized single versus double embryo transfer: obstetric and paediatric outcome and a cost-effectiveness analysis. Hum Reprod 2006;21:210-216. (Pubitemid 41794313)
7. Kovalevsky G, Patrizio P. High rates of embryo wastage with use of assisted reproductive technology: a look at the trends between 1995 and 2001 in the United States. Fertil Steril 2005;84:325-330. (Pubitemid 41125252)
8. Leese HJ. Metabolism of the preimplantation embryo: 40 years on. Reproduction 2012; doi: 10.1530/REP-11-0484.
9. Mastenbroek S, Twisk M, van der Veen F, Repping S. Preimplantation genetic screening: a systematic review and meta-analysis of RCTs. Hum Reprod Update 2011;17:454-466.
10. Nagy ZP, Sakkas D, Behr B. Non-invasive assessment of embryo viability by metabolomic profiling of culture media ('metabolomics'). Reprod Biomed Online 2008;17:502-507.
11. Nagy ZP, Jones-Colon S, Roos P, Botros L, Greco E, Dasig J, Behr B. Metabolomic assessment of oocyte viability. Reprod Biomed Online 2009;18:219-225.
12. Pandian Z, Bhattacharya S, Ozturk O, Serour G, Templeton A. Number of embryos for transfer following in-vitro fertilisation or intra-cytoplasmic sperm injection. Cochrane Database Syst Rev 2009;15:CD003416.
13. Paternot G, Devroe J, Debrock S, D'Hooghe TM, Spiessens C. Intra-and inter-observer analysis in the morphological assessment of early-stage embryos. Reprod Biol Endocrin 2009;29:105-110.
14. Scott RT, Seli E, Miller K, Sakkas D, Scott K, Burns DH. Noninvasive metabolomic profiling of human embryo culture media using Raman spectroscopy predicts embryonic reproductive potential: a prospective blinded pilot study. Fertil Steril 2008;90:77-83.
15. Seli E, Sakkas D, Scott R, Kwok SC, Rosendhal SM, Burns DH. Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization. Fertil Steril 2007; 88:1350-1357. (Pubitemid 350030104)
16. Seli E, Robert C, Sirard MA. OMICS in assisted reproduction: possibilities and pitfalls. Mol Hum Reprod 2010a;16:513-530.
17. Seli E, Vergouw CG, Morita H, Botros LL, Roos P, Lambalk CB, Yamashita N, Kato O, Sakkas D. Noninvasive metabolomic profiling as an adjunct to morphology for noninvasive embryo assessment in women undergoing single embryo transfer. Fertil Steril 2010b; 94:535-542.
18. Seli E, Bruce C, Botros LL, Henson M, Roos P, Judge K, Hardarson T, Ahlstrom A, Harrison P, Henman M et al. Receiver operating characteristic (ROC) analysis of day 5 morphology grading and metabolomics viability Score on predicting implantation outcome. J Assist Reprod Genet 2011;28:137-144.
19. Sfontouris IA, Lainas GT, Sakkas D, Iliadis GS, Anagnostara K, Zorzovilis IZ, Petsas GK, Lainas TG. Assessment of embryo selection using non-invasive metabolomic analysis as an adjunct to morphology indicates improvement in implantation and fetal cardiac activity rates. Hum Reprod 2011; 26(Suppl.1):i86.
20. Thurin A, Hausken J, HillensjöT, Jablanowska B, Pinborg A, Strandell A, Bergh C. Elective single-embryo transfer versus double-embryo transfer in in-vitro fertilization. N Engl J Med 2004; 351:2392-2402. (Pubitemid 40485991)
21. Vergouw CG, Botros LL, Roos P, Lens JW, Schats R, Hompes PGA, Burns DH, Lambalk CB. Metabolomic profiling by near infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection. Hum Reprod 2008; 23:1499-1504. (Pubitemid 351809705)
22. Vergouw CG, Botros LL, Judge K, Henson M, Roos P, Kostelijk EH, Schats R, Twisk JWR, Hompes PGA, Sakkas D et al. Non-invasive viability assessment of day 4 frozen-thawed human embryos by near infrared spectroscopy. Reprod Biomed Online 2011;23: 769-776.