Generation and characterization of novel cytochrome P450 Cyp2c gene cluster knockout and CYP2C9 humanized mouse lines

Molecular Pharmacology

Volumn 82, Issue 6, 2012, Pages 1022-1029

  Scheer, Nico ^a   Kapelyukh, Yury ^b   Chatham, Lynsey ^c   Rode, Anja ^a   Buechel, Sandra ^a   Wolf, C Roland ^b,c  

^a TaconicArtemis   (Germany)

^b CXR Biosciences Ltd   (United Kingdom)

^c UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BENZBROMARONE; CYTOCHROME P450 2C; CYTOCHROME P450 2C9; DICLOFENAC; FLUCONAZOLE; FLUOXETINE; FLUVOXAMINE; SULFAPHENAZOLE; TOLBUTAMIDE;

ANIMAL CELL; ANIMAL EXPERIMENT; AREA UNDER THE CURVE; ARTICLE; CONTROLLED STUDY; DRUG BLOOD LEVEL; DRUG DISPOSITION; DRUG HYDROXYLATION; DRUG INHIBITION; DRUG METABOLISM; ENZYME ACTIVITY; ENZYME METABOLISM; ENZYME SUBSTRATE; FEMALE; GENE CLUSTER; GENE DELETION; GENE EXPRESSION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; LIVER; MAXIMUM PLASMA CONCENTRATION; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; WILD TYPE;

ANIMALS; ARYL HYDROCARBON HYDROXYLASES; CYTOCHROME P-450 ENZYME SYSTEM; HUMANS; HYDROXYLATION; LIVER; METABOLIC DETOXICATION, DRUG; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; MULTIGENE FAMILY; TOLBUTAMIDE;

EID: 84869760907     PISSN: 0026895X     EISSN: 15210111     Source Type: Journal    
DOI: 10.1124/mol.112.080036     Document Type: Article

References (35)

1. Aasheim ET, Hofsø D, Hjelmesaeth J, Birkeland KI, and Bøhmer T (2008) Vitamin status in morbidly obese patients: a cross-sectional study. Am J Clin Nutr 87:362-369. (Pubitemid 351226072)
2. Al-Dosari MS, Knapp JE, and Liu D (2006) Activation of human CYP2C9 promoter and regulation by CAR and PXR in mouse liver. Mol Pharm 3:322-328. (Pubitemid 44021438)
3. Baillie TA and Rettie AE (2011) Role of biotransformation in drug-induced toxicity: influence of intra- and inter-species differences in drug metabolism. Drug Metab Pharmacokinet 26:15-29.
4. Chen Y, Ferguson SS, Negishi M, and Goldstein JA (2004) Induction of human CYP2C9 by rifampicin, hyperforin, and phenobarbital is mediated by the pregnane X receptor. J Pharmacol Exp Ther 308:495-501. (Pubitemid 38134210)
5. Cheung C, Ma X, Krausz KW, Kimura S, Feigenbaum L, Dalton TP, Nebert DW, Idle JR, and Gonzalez FJ (2005) Differential metabolism of 2-amino-1-methyl-6- phenylimidazolsqb]4,5-brsqb]pyridine (PhIP) in mice humanized for CYP1A1 and CYP1A2. Chem Res Toxicol 18:1471-1478. (Pubitemid 41361721)
6. Cheung C, Yu AM, Chen CS, Krausz KW, Byrd LG, Feigenbaum L, Edwards RJ, Waxman DJ, and Gonzalez FJ (2006) Growth hormone determines sexual dimorphism of hepatic cytochrome P450 3A4 expression in transgenic mice. J Pharmacol Exp Ther 316:1328-1334. (Pubitemid 43345309)
7. Corchero J, Granvil CP, Akiyama TE, Hayhurst GP, Pimprale S, Feigenbaum L, Idle JR, and Gonzalez FJ (2001) The CYP2D6 humanized mouse: effect of the human CYP2D6 transgene and HNF4α on the disposition of debrisoquine in the mouse. Mol Pharmacol 60:1260-1267. (Pubitemid 33144897)
8. Dragin N, Uno S, Wang B, Dalton TP, and Nebert DW (2007) Generation of 'humanized' hCYP1A1-1A2-Cyp1a1/1a2(minus]/minus]) mouse line. Biochem Biophys Res Commun 359:635-642. (Pubitemid 46898998)
9. Faiz S, Panunti B, and Andrews S (2007) The epidemic of vitamin D deficiency. J La State Med Soc 159:17-20; quiz 20,55.
10. Ferguson SS, LeCluyse EL, Negishi M, and Goldstein JA (2002) Regulation of human CYP2C9 by the constitutive androstane receptor: discovery of a new distal binding site. Mol Pharmacol 62:737-746. (Pubitemid 36329394)
11. Foreman JE, Blizard DA, Gerhard G, Mack HA, Lang DH, Van Nimwegen KL, Vogler GP, Stout JT, Shihabi ZK, Griffith JW, et al. (2005) Serum alkaline phosphatase activity is regulated by a chromosomal region containing the alkaline phosphatase 2 gene (Akp2) in C57BL/6J and DBA/2J mice. Physiol Genomics 23:295-303. (Pubitemid 44437965)
12. Gerbal-Chaloin S, Daujat M, Pascussi JM, Pichard-Garcia L, Vilarem MJ, and Maurel P (2002) Transcriptional regulation of CYP2C9 gene. Role of glucocorticoid receptor and constitutive androstane receptor. J Biol Chem 277:209-217. (Pubitemid 34952047)
13. Gonzalez FJ and Yu AM (2006) Cytochrome P450 and xenobiotic receptor humanized mice. Annu Rev Pharmacol Toxicol 46:41-64. (Pubitemid 43271183)
14. Guengerich FP (2003) Cytochromes P450, drugs, and diseases. Mol Interv 3:194-204.
15. Guengerich FP (2008) Cytochrome p450 and chemical toxicology. Chem Res Toxicol 21:70-83.
16. Hasegawa M, Kapelyukh Y, Tahara H, Seibler J, Rode A, Krueger S, Lee DN, Wolf CR, and Scheer N (2011) Quantitative prediction of human pregnane X receptor and cytochrome P450 3A4 mediated drug-drug interaction in a novel multiple humanized mouse line. Mol Pharmacol 80:518-528.
17. Hogan BLM, Beddington RSP, Costantini F, and Lacy E (1994) in Manipulating the Mouse Embryo: A Laboratory Manual, pp 253-289, Cold Spring Harbor Laboratory Press, Plainview, NY.
18. Jiang Z, Dalton TP, Jin L, Wang B, Tsuneoka Y, Shertzer HG, Deka R, and Nebert DW (2005) Toward the evaluation of function in genetic variability: characterizing human SNP frequencies and establishing BAC-transgenic mice carrying the human CYP1A1-CYP1A2 locus. Hum Mutat 25:196-206. (Pubitemid 40216616)
19. Lee G and Saito I (1998) Role of nucleotide sequences of loxP spacer region in Cre-mediated recombination. Gene 216:55-65. (Pubitemid 28389102)
20. Löfgren S, Baldwin RM, Hiratsuka M, Lindqvist A, Carlberg A, Sim SC, Schülke M, Snait M, Edenro A, Fransson-Steen R, et al. (2008) Generation of mice transgenic for human CYP2C18 and CYP2C19: characterization of the sexually dimorphic gene and enzyme expression. Drug Metab Dispos 36:955-962. (Pubitemid 351574895)
21. Michaelis UR, Xia N, Barbosa-Sicard E, Falck JR, and Fleming I (2008) Role of cytochrome P450 2C epoxygenases in hypoxia-induced cell migration and angiogenesis in retinal endothelial cells. Invest Ophthalmol Vis Sci 49:1242-1247.
22. Nelson DR, Zeldin DC, Hoffman SM, Maltais LJ, Wain HM, and Nebert DW (2004) Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternativesplice variants. Pharmacogenetics 14:1-18. (Pubitemid 38183957)
23. Paine MF, Hart HL, Ludington SS, Haining RL, Rettie AE, and Zeldin DC (2006) The human intestinal cytochrome P450 "pie." Drug Metab Dispos 34:880-886.
24. Powley MW, Frederick CB, Sistare FD, and DeGeorge JJ (2009) Safety assessment of drug metabolites: implications of regulatory guidance and potential application of genetically engineered mouse models that express human P450s. Chem Res Toxicol 22:257-262.
25. Rettie AE and Jones JP (2005) Clinical and toxicological relevance of CYP2C9: drug-drug interactions and pharmacogenetics. Annu Rev Pharmacol Toxicol 45:477-494. (Pubitemid 40267790)
26. Scheer N, Kapelyukh Y, McEwan J, Beuger V, Stanley LA, Rode A, and Wolf CR (2012) Modeling human cytochrome P450 2D6 metabolism and drug-drug interaction by a novel panel of knockout and humanized mouse lines. Mol Pharmacol 81:63-72.
27. Scheer N, Ross J, Rode A, Zevnik B, Niehaves S, Faust N, and Wolf CR (2008) A novel panel of mouse models to evaluate the role of human pregnane X receptor and constitutive androstane receptor in drug response. J Clin Invest 118:3228-3239.
28. Stubbins MJ, Harries LW, Smith G, Tarbit MH, and Wolf CR (1996) Genetic analysis of the human cytochrome P450 CYP2C9 locus. Pharmacogenetics 6:429-439. (Pubitemid 26380099)
29. Tang W, Stearns RA, Bandiera SM, Zhang Y, Raab C, Braun MP, Dean DC, Pang J, Leung KH, Doss GA, et al. (1999) Studies on cytochrome P-450-mediated bioactivation of diclofenac in rats and in human hepatocytes: identification of glutathione conjugated metabolites. Drug Metab Dispos 27:365-372. (Pubitemid 29114244)
30. van Herwaarden AE, Wagenaar E, van der Kruijssen CM, van Waterschoot RA, Smit JW, Song JY, van der Valk MA, van Tellingen O, van der Hoorn JW, Rosing H, Beijnen JH, and Schinkel AH. (2007) Knockout of cytochrome P450 3A yields new mouse models for understanding xenobiotic metabolism. J Clin Invest 117:3583-3592. (Pubitemid 350097013)
31. van Waterschoot RA, Rooswinkel RW, Wagenaar E, van der Kruijssen CM, van Herwaarden AE, and Schinkel AH (2009) Intestinal cytochrome P450 3A plays an important role in the regulation of detoxifying systems in the liver. FASEB J 23:224-231.
32. van Waterschoot RA, van Herwaarden AE, Lagas JS, Sparidans RW, Wagenaar E, van der Kruijssen CM, Goldstein JA, Zeldin DC, Beijnen JH, and Schinkel AH (2008) Midazolam metabolism in cytochrome P450 3A knockout mice can be attributed to up-regulated CYP2C enzymes. Mol Pharmacol 73:1029-1036. (Pubitemid 351397908)
33. Webler AC, Popp R, Korff T, Michaelis UR, Urbich C, Busse R, and Fleming I (2008) Cytochrome P450 2C9-induced angiogenesis is dependent on EphB4. Arterioscler Thromb Vasc Biol 28:1123-1129.
34. Williams JA, Hyland R, Jones BC, Smith DA, Hurst S, Goosen TC, Peterkin V, Koup JR, and Ball SE. (2004) Drug-drug interactions for UDP- glucuronosyltransferase substrates: a pharmacokinetic explanation for typically observed low exposure (AUCi/AUC) ratios. Drug Metab Dispos 32:1201-1208. (Pubitemid 39410902)
35. Zhang Y, Buchholz F, Muyrers JP, and Stewart AF (1998) A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet 20:123-128. (Pubitemid 28455443)