Preparation of polyclonal antibodies and development of a direct competitive enzyme-linked immunosorbent assay to detect residues of phenylethanolamine a in urine samples

Journal of Agricultural and Food Chemistry

Volumn 60, Issue 46, 2012, Pages 11618-11624

  Bai, Yuhui ^a   Liu, Zhihong ^a   Bi, Yanfeng ^a   Wang, Xia ^a   Jin, Yinzhen ^a   Sun, Lei ^a   Wang, Hejia ^a   Zhang, Congmin ^a   Xu, Shixin ^a  

^a China Institute of Veterinary Drug Control   (China)

Author keywords

agonists; ELISA; phenylethanolamine A; polyclonal antibody; residues

Indexed keywords

COEFFICIENT OF VARIATION; CROSS-REACTIVITY; DETECTION LIMITS; ELISA; ENZYME LINKED IMMUNOSORBENT ASSAY; HIGH SENSITIVITY; LARGE GROUPS; LIMIT OF DETECTION; PHENYLETHANOLAMINE A; POLYCLONAL ANTIBODY; RACTOPAMINE; REAL SAMPLES; RECOVERY RATE; RESIDUES; UPLC-MS/MS; URINE SAMPLE;

CHEMICAL DETECTION;

ANTIBODIES;

ANTIBODY; BETA ADRENERGIC RECEPTOR STIMULATING AGENT; DRUG RESIDUE; ETHANOLAMINE;

ANIMAL; ARTICLE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; EVALUATION; GROWTH, DEVELOPMENT AND AGING; METHODOLOGY; RABBIT; SWINE; URINE;

ADRENERGIC BETA-AGONISTS; ANIMALS; ANTIBODIES; DRUG RESIDUES; ENZYME-LINKED IMMUNOSORBENT ASSAY; ETHANOLAMINE; RABBITS; SWINE;

EID: 84869475519     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf3036066     Document Type: Article

References (21)

1. Crome, P. K.; McKeith, F. K.; Carr, T. R.; Jones, D. J.; Mowrey, D. H.; Cannon, J. E. Effect of ractopmine on growth performance, carcass composition and cutting yields of pogs slaughtered at 107 and 125 kg J. Anim. Sci. 1996, 74, 709-716
2. Watkins, L. E.; Jones, D. J.; Mowrey, D. H.; Anderson, D. B.; Veenhuizen, E. L. The effect of various levels of ractopamine hydrochloride on the performance and carcass characteristics of finishing swine J. Anim. Sci. 1990, 68, 3588-3595
3. Engeseth, N. J.; Lee, K. O.; Bergen, W. G.; Helferich, W. H.; Knudson, B. K.; Merkel, R. A. Fatty acid profiles of lipid depots and cholesterol concentration in muscle tissue of finishing feed ractopamine J. Food Sci. 1992, 57, 1060-1062
4. Xiong, Y. L.; Gower, M. J.; Li, C.; Elmore, C. A.; Cromwell, G. L.; Lindemann, M. D. Effect of dietary ractopamine on tenderness and postmortem protein degradation of pork muscle Meat Sci. 2006, 73, 600-604
5. Brambilla, G.; Cenci, T.; Franconi, F. Clinical and pharmacological profile in a clenbuterol epidemic poisoning of contaminated beefmeat in Italy Toxicol. Lett. 2000, 114, 47-53
6. 5--luminol chemiluminescence detection J. Agric. Food Chem. 2007, 55, 4949-4956
7. Turberg, M. P.; Macy, T. D.; Lewis, J. J.; Coleman, M. R. Determination of ractopamine hydrochloride in swine, cattle, and turkey feeds by liquid chromatography with coulometric detection J. AOAC Int. 1994, 77, 840-847
8. Shishani, E.; Chai, S. C.; Jamokha, S.; Aznar, G.; Hoffman, M. K. Determination of ractopamine in animal tissues by liquid chromatography- fluorescence and liquid chromatography/tandem mass spectrometry Anal. Chim. Acta 2003, 483, 137-145
9. Antignac, J. P.; Marchand, P.; Bizec, B.; Le Andre, F. Identification of ractopamine residues in tissue and urine samples at ultra-trace level using liquid chromatographypositive electrospray tandem mass spectrometry J. Chromatogr., B 2002, 774, 59-66
10. Churchwell, M. I.; Holder, C. L.; Little, D.; Preece, S.; Smith, D. J.; Doerge, D. R. Liquid chromatography/electrospray tandem mass spectrometric analysis of incurred ractopamine residues in livestock tissues Rapid Commun. Mass Spectrom. 2002, 16, 1261-1265
11. Wang, J. P.; Li, X. W.; Zhang, W.; Shen, J. Z. Development of immunoaffinity sample-purification for GC-MS analysis of ractopamine in swine tissue Chromatographia 2006, 64, 613-617
12. Haasnoot, W.; Cazemier, G.; Stouten, P.; Kemmers-Voncken, A. Immunochemical approaches to the analysis of β-agonistic drugs. Immunoassays for Residue Analysis; American Chemical Society: Washington, DC, USA, 1996, Chapter 5, pp 60-73.
13. Degand, G.; Bernes-Duyckaerts, A.; Maghuin-Rogister, G. Determination of clenbuterol in bovine tissues and urine by enzyme immunoassay J. Agric. Food Chem. 1992, 40, 70-75
14. Sun, Z. W.; Yan, X. F. The detection of phenylethanolamine A in porcine muscle tissue using liquid chromatography tandem mass spectrometry method Chin. J. Vet. Med. 2011, 47, 72-74
15. Dong, Y. C.; Xia, X.; Wang, X.; Ding, S. Y.; Li, X. W.; Zhang, S. X. Validation of an ultra-performance liquid chromatography-tandem mass spectrometry method for determination of ractopamine: Application to residue depletion study in swine Food Chem. 2011, 127, 327-332
16. Hermanson, G. T. Bioconjugate Techniques; Academic Press: San Diego, CA, 1996.
17. Xing, Q. Y.; Xu, R. Basic Organic Chemistry; Advanced Education Press: Beijing, China, 2005.
18. Yang, L. G.; Hu, S. Y.; Wei, P. H.; Guo, A. Z. Enzyme Immunoassay Technology; Nanjing University Press: Beijing, China, 1998; pp 272-273.
19. Jiang, J. Q.; Wang, Z. L.; Zhang, H. T.; Zhang, X. J.; Liu, X. Y.; Wang, S. H. Monoclonal antibody-based ELISA and colloidal gold immunoassay for detecting 19-nortestosterone residue in animal tissues J. Agric. Food Chem. 2011, 59, 9763-9769
20. Zhang, Y.; Wang, F. X.; Fang, L.; Wang, S.; Fang, G. Z. Rapid determination of ractopamine residues in edible animal products by enzyme-linked immunosorbent assay: Development and investigation of matrix effects J. Biomed. Biotechnol. 2009, 2009, 579175
21. Crescenzi, C.; Bayoudh, S.; Cormack, P. A. G.; Klein, T.; Ensing, K. Determination of clenbuterol in bovine liver by combining matrix solid-phase dispersion and molecularly imprinted solid-phase extraction followed by liquid chromatography/electrospray ion trap multiple-stage mass spectrometry Anal. Chem. 2001, 73, 2171-2177