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Bioorganic and Medicinal Chemistry Letters
Volumn 22, Issue 23, 2012, Pages 7123-7126
T-type Ca2+ channel blocker, KYS05047 induces G1 phase cell cycle arrest by decreasing intracellular Ca2+ levels in human lung adenocarcinoma A549 cells
Author keywords

3,4 Dihydroquinazoline; Cell cycle arrest; Intracellular Ca2+ level; T type Ca2+ channel blocker
Indexed keywords

4 (BENZYLCARBAMOYLMETHYL) 2 (BIPHENYL 4 YLAMINO) 3 (5 TERT BUTYLOXYCARBAMOYL 1 PENTYL) 3,4 DIHYDROQUINAZOLINE; CALCIUM CHANNEL BLOCKING AGENT RECEPTOR; CALCIUM CHANNEL T TYPE; CYCLIN D1; CYCLIN DEPENDENT KINASE 2; CYCLIN DEPENDENT KINASE 4; CYCLIN DEPENDENT KINASE INHIBITOR 1B; CYCLIN E; KYS 05047; PACLITAXEL; QUINAZOLINE DERIVATIVE; UNCLASSIFIED DRUG; 4-(BENZYLCARBAMOYLMETHYL)-2-(BIPHENYL-4-YLAMINO)-3-(5-TERT-BUTYLOXYCARBAMOYL-1-P ENTYL)-3,4-DIHYDROQUINAZOLINE; CALCIUM; CALCIUM CHANNEL BLOCKING AGENT;
EID: 84869085153     PISSN: 0960894X     EISSN: 14643405     Source Type: Journal    
DOI: 10.1016/j.bmcl.2012.09.076     Document Type: Article
Times cited : (28)
References (27)
  • 15
    • 84855323238 scopus 로고    scopus 로고
    • Appendix 3, Appendix 3B. Trypan blue exclusion test: A549 cells, cultured in the presence of KYS05047 at various concentrations (0, 4, 6, 8, 10, and 12 μM) for different times (0, 6, 12, 24, 36, and 48 h), were collected by centrifugation and resuspended with 0.4% trypan blue solution. And then incubate 3 min at room temperature and viable cells (unstained cells) were counted using hemocytometer
    • W. Strober Curr. Protoc. Immunol. 1 2001 2 Appendix 3, Appendix 3B. Trypan blue exclusion test: A549 cells, cultured in the presence of KYS05047 at various concentrations (0, 4, 6, 8, 10, and 12 μM) for different times (0, 6, 12, 24, 36, and 48 h), were collected by centrifugation and resuspended with 0.4% trypan blue solution. And then incubate 3 min at room temperature and viable cells (unstained cells) were counted using hemocytometer
    • (2001) Curr. Protoc. Immunol. , vol.1 , pp. 2
    • Strober, W.1
  • 16
    • 84870441818 scopus 로고
    • Cell cycle analysis: cells were treated with various concentrations of KYS05047, and then washed with 1 ml phosphate-buffered saline (PBS), fixed in 70% ice-cold ethanol and kept in a freezer overnight. The fixed cells were centrifuged, washed twice with PBS and re-suspended in PBS containing 50 mg/ml PI and 100 μg/ml DNase-free RNase A. The cell suspension, which was hidden from light, was incubated for 30 min and analyzed using the fluorescence- activated cell sorting cater-plus FACS (Becton Dickinson Co, Heidelberg, Germany)
    • K.W. Lee, H.J. Kim, Y.S. Lee, H.J. Park, J.W. Choi, J. Ha, and K.T. Lee Carcinogenesis 2007 1928 28 Cell cycle analysis: cells were treated with various concentrations of KYS05047, and then washed with 1 ml phosphate-buffered saline (PBS), fixed in 70% ice-cold ethanol and kept in a freezer overnight. The fixed cells were centrifuged, washed twice with PBS and re-suspended in PBS containing 50 mg/ml PI and 100 μg/ml DNase-free RNase A. The cell suspension, which was hidden from light, was incubated for 30 min and analyzed using the fluorescence-activated cell sorting cater-plus FACS (Becton Dickinson Co, Heidelberg, Germany)
    • (1928) Carcinogenesis , vol.2007 , pp. 28
    • Lee, K.W.1    Kim, H.J.2    Lee, Y.S.3    Park, H.J.4    Choi, J.W.5    Ha, J.6    Lee, K.T.7
  • 22
    • 0034015954 scopus 로고    scopus 로고
    • 2+ concentration was measured using Flou-4 calcium assay kit (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions
    • 2+ concentration was measured using Flou-4 calcium assay kit (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's instructions
    • (2000) Cell Calcium , vol.27 , pp. 97
    • Gee, K.R.1    Brown, K.A.2    Chen, W.N.3    Bishop-Stewart, J.4    Gray, D.5    Johnson, I.6
  • 27
    • 84855659193 scopus 로고    scopus 로고
    • A549 xenograft model: A549 cells (5 × 106 cells/mice) were injected subcutaneously into the left flank of BALB/cnu/nu mice. Mice were separated into four groups (n = 7). After 8 days when average tumor volume reached approximately 50-100 mm3, paclitaxel (20 mg/kg; twice/week; ip) and KYS05047 (2 or 10 mg/kg; 5 times/week; po) were administered for 21 days. Tumor lengths and widths were measured using a caliper and tumor volume was calculated as 1/2 × length × width2 until animal sacrifice. Control animals and the animals treated with paclitaxel or KYS05047 were euthanized after 21 days and the tumors were excised. Tumor weights of each group were measured and the representative tumors were photographed
    • H.B. Kang, H.K. Rim, J.Y. Park, H.W. Choi, D.L. Choi, J.H. Seo, K.S. Chung, G. Huh, J. Kim, D.J. Choo, K.T. Lee, and J.Y. Lee Bioorg. Med. Chem. Lett. 22 2012 1198 A549 xenograft model: A549 cells (5 × 106 cells/mice) were injected subcutaneously into the left flank of BALB/cnu/nu mice. Mice were separated into four groups (n = 7). After 8 days when average tumor volume reached approximately 50-100 mm3, paclitaxel (20 mg/kg; twice/week; ip) and KYS05047 (2 or 10 mg/kg; 5 times/week; po) were administered for 21 days. Tumor lengths and widths were measured using a caliper and tumor volume was calculated as 1/2 × length × width2 until animal sacrifice. Control animals and the animals treated with paclitaxel or KYS05047 were euthanized after 21 days and the tumors were excised. Tumor weights of each group were measured and the representative tumors were photographed
    • (2012) Bioorg. Med. Chem. Lett. , vol.22 , pp. 1198
    • Kang, H.B.1    Rim, H.K.2    Park, J.Y.3    Choi, H.W.4    Choi, D.L.5    Seo, J.H.6    Chung, K.S.7    Huh, G.8    Kim, J.9    Choo, D.J.10    Lee, K.T.11    Lee, J.Y.12

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.