Expression and purification of a chimeric protein consisting of the ectodomains of M and GP5 proteins of porcine reproductive and respiratory syndrome virus (PRRSV)

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 911, Issue , 2012, Pages 43-48

  Hu, Jianzhong ^a   Ni, Yanyan ^b   Meng, X J ^b   Zhang, Chenming ^a  

^a Virginia Polytechnic Institute and State University   (United States)

^b CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

Author keywords

Heparan sulfate; Porcine reproductive and respiratory syndrome virus (PRRSV); Protein purification; Protein refolding; Vaccine

Indexed keywords

ANION EXCHANGE CHROMATOGRAPHY; CELLULAR RECEPTORS; CHIMERIC PROTEINS; E. COLI; ECONOMIC LOSS; HEPARAN SULFATES; IMMOBILIZED METAL ION AFFINITY CHROMATOGRAPHIES; INCLUSION BODIES; INFECTIOUS DISEASE; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS (PRRSV); PROTEIN PURIFICATION; PROTEIN REFOLDING; PURIFIED PROTEIN; REFOLDING; SUBUNIT VACCINE; VIRUS INFECTION;

AFFINITY CHROMATOGRAPHY; DIALYSIS; ESCHERICHIA COLI; LOSSES; PURIFICATION; VACCINES; VIRUSES;

PROTEINS;

CHIMERIC PROTEIN; HEPARAN SULFATE; VIRUS GLYCOPROTEIN; VIRUS VACCINE;

ANION EXCHANGE CHROMATOGRAPHY; ANTIGEN SPECIFICITY; ARTERIVIRUS; ARTICLE; BROTH DILUTION; CONTROLLED STUDY; DIALYSIS; ESCHERICHIA COLI; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; NONHUMAN; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN REFOLDING; SWINE;

BLOTTING, WESTERN; CHROMATOGRAPHY, ION EXCHANGE; ESCHERICHIA COLI; HEPARITIN SULFATE; HISTIDINE; OLIGOPEPTIDES; PROTEIN REFOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; VIRAL ENVELOPE PROTEINS; VIRAL MATRIX PROTEINS;

PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; SUIDAE; SUS;

EID: 84868686942     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2012.10.014     Document Type: Article

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