A highly stable protein chimera built from fragments of different folds

Protein Engineering, Design and Selection

Volumn 25, Issue 11, 2012, Pages 699-703

  Shanmugaratnam, Sooruban ^a   Eisenbeis, Simone ^a   Höcker, Birte ^a  

^a MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY   (Germany)

Author keywords

( )8 barrel; chimera; flavodoxin like; hybrid proteins; protein design

Indexed keywords

CHEMICAL DENATURATION; CHIMERA; CHIMERIC PROTEINS; DESIGN STRATEGIES; FLAVODOXIN-LIKE; NOVEL FOLDS; PROTEIN DESIGN; PROTEIN FOLDS;

CHEMICAL STABILITY; CURRICULA; PROTEIN FOLDING;

PROTEINS;

PROTEIN;

CHIMERA; CONFERENCE PAPER; ESCHERICHIA COLI; GENE; MOLECULAR EVOLUTION; MOLECULAR WEIGHT; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE;

BACTERIAL PROTEINS; CLONING, MOLECULAR; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HOT TEMPERATURE; MODELS, MOLECULAR; PROPIONIBACTERIUM; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS; THERMOTOGA MARITIMA;

EID: 84868531723     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs074     Document Type: Conference Paper

References (20)

1. Bharat, T. A., Eisenbeis, S., Zeth, K. and Höcker, B. (2008) Proc. Natl Acad. Sci. USA, 105, 9942-9947
2. Carstensen, L., Zoldak, G., Schmid, F. X. and Sterner, R. (2012) Biochemistry Claren, J., Malisi, C., Höcker, B. and Sterner, R. (2009) Proc. Natl Acad. Sci. USA, 106, 3704-3709
3. Eisenbeis, S. and Höcker, B. (2010) J. Pept. Sci., 16, 538-544
4. Eisenbeis, S., Proffitt, W., Coles, M., Truffault, V., Shanmugaratnam, S., Meiler, J. and Höcker, B. (2012) J. Am. Chem. Soc., 134, 4019-4022
5. Fortenberry, C., Bowman, E. A., Proffitt, W., Dorr, B., Combs, S., Harp, J., Mizoue, L. and Meiler, J. (2011) J. Am. Chem. Soc., 133, 18026-18029
6. Grishin, N. V. (2001) J. Struct. Biol., 134, 167-185
7. Höcker, B., Beismann-Driemeyer, S., Hettwer, S., Lustig, A. and Sterner, R. (2001) Nat. Struct. Biol., 8, 32-36
8. Höcker, B., Schmidt, S. and Sterner, R. (2002) FEBS Lett., 510, 133-135
9. Höcker, B., Claren, J. and Sterner, R. (2004) Proc. Natl Acad. Sci. USA, 101, 16448-16453
10. Höcker, B., Lochner, A., Seitz, T., Claren, J. and Sterner, R. (2009) Biochemistry, 48, 1145-1147
11. Holm, L. and Sander, C. (1993) J. Mol. Biol., 233, 123-138
12. Huynen, M. A. and Bork, P. (1998) Proc. Natl Acad. Sci. USA, 95, 5849-5856
13. Lang, D., Thoma, R., Henn-Sax, M., Sterner, R. and Wilmanns, M. (2000) Science, 289, 1546-1550
14. Lynch, M. and Conery, J. S. (2000) Science, 290, 1151-1155
15. Mancia, F., Keep, N. H., Nakagawa, A., Leadlay, P. F., McSweeney, S., Rasmussen, B., Bosecke, P., Diat, O. and Evans, P. R. (1996) Structure, 4, 339-350
16. Sali, A. and Blundell, T. L. (1993) J. Mol. Biol., 234, 779-815
17. Seitz, T., Bocola, M., Claren, J. and Sterner, R. (2007) J. Mol. Biol., 372, 114-129
18. Söding, J. and Lupas, A. N. (2003) Bioessays, 25, 837-846
19. Sterner, R. and Höcker, B. (2005) Chem. Rev., 105, 4038-4055
20. Stock, A. M., Robinson, V. L. and Goudreau, P. N. (2000) Annu. Rev. Biochem., 69, 183-215