TetR-based gene regulation systems for Francisella tularensis

Applied and Environmental Microbiology

Volumn 78, Issue 19, 2012, Pages 6883-6889

  LoVullo, Eric D ^a   Miller, Cheryl N ^a   Pavelka, Martin S ^b   Kawula, Thomas H ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; LUMINESCENCE; TRANSCRIPTION;

ANHYDROTETRACYCLINE; ETIOLOGICAL AGENT; EXPRESSION SYSTEM; FRANCISELLA TULARENSIS; GENE-REGULATION; GENES EXPRESSION; GENETIC TOOLS; INDUCIBLE SYSTEMS; PROMOTER SEQUENCES; REGULATED PROMOTERS;

PROTEINS;

EID: 84868322922     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01679-12     Document Type: Article

References (39)

1. Akerley BJ, et al. 1998. Systematic identification of essential genes by in vitro mariner mutagenesis. Proc. Natl. Acad. Sci. U. S. A. 95: 8927-8932.
2. Ausubel FM, et al. 1987. Current protocols in molecular biology. John Wiley and Sons, Inc, New York, NY.
3. Bakshi CS, et al. 2006. Superoxide dismutase B gene (sodB)-deficient mutants of Francisella tularensis demonstrate hypersensitivity to oxidative stress and attenuated virulence. J. Bacteriol. 188: 6443-6448.
4. Beare PA, et al. 2011. Dot/Icm type IVB secretion system requirements for Coxiella burnetii growth in human macrophages. mBio 2: e00175-e00111. doi: 10.1128/mBio.00175-11.
5. Benson RE, Gottlin EB, Christensen DJ, Hamilton PT. 2003. Intracellular expression of peptide fusions for demonstration of protein essentiality in bacteria. Antimicrob. Agents Chemother. 47: 2875-2881.
6. Bertram R, Hillen W. 2008. The application of Tet repressor in prokaryotic gene regulation and expression. Microb. Biotechnol. 1: 2-16.
7. Bina XR, Miller MA, Bina JE. 2010. Construction of a bioluminescence reporter plasmid for Francisella tularensis. Plasmid 64: 156-161.
8. Cabelli RJ, Chen L, Tai PC, Oliver DB. 1988. SecA protein is required for secretory protein translocation into E. coli membrane vesicles. Cell 55: 683-692.
9. Caspers M, Freudl R. 2008. Corynebacterium glutamicum possesses two secA homologous genes that are essential for viability. Arch. Microbiol. 189: 605-610.
10. Chamberlain RE. 1965. Evaluation of live tularemia vaccine prepared in a chemically defined medium. Appl. Microbiol. 13: 232-235.
11. Cunningham K, Wickner W. 1989. Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 86: 8630-8634.
12. de Lorenzo V, Timmis KN. 1994. Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5- and Tn10-derived minitransposons. Methods Enzymol. 235: 386-405.
13. Dennis DT, et al. 2001. Tularemia as a biological weapon: medical and public health management. JAMA 285: 2763-2773.
14. Ehrt S, et al. 2005. Controlling gene expression in mycobacteria with anhydrotetracycline and Tet repressor. Nucleic Acids Res. 33: e21. doi: 10.1093/nar/gni013.
15. Ericsson M, Golovliov I, Sandstrom G, Tarnvik A, Sjostedt A. 1997. Characterization of the nucleotide sequence of the groE operon encoding heat shock proteins chaperone-60 and -10 of Francisella tularensis and determination of the T-cell response to the proteins in individuals vaccinated with F. tularensis. Infect. Immun. 65: 1824-1829.
16. Fagan RP, Fairweather NF. 2011. Clostridium difficile has two parallel and essential Sec secretion systems. J. Biol. Chem. 286: 27483-27493.
17. Forsyth RA, et al. 2002. A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol. Microbiol. 43: 1387-1400.
18. Fuller JR, et al. 2008. RipA, a cytoplasmic membrane protein conserved among Francisella species, is required for intracellular survival. Infect. Immun. 76: 4934-4943.
19. Fuller JR, Kijek TM, Taft-Benz S, Kawula TH. 2009. Environmental and intracellular regulation of Francisella tularensis ripA. BMC Microbiol. 9: 216. doi: 10.1186/1471-2180-9-216.
20. Gallagher LA, et al. 2007. A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc. Natl. Acad. Sci. U. S. A. 104: 1009-1014.
21. Gossen M, Bujard H. 1993. Anhydrotetracycline, a novel effector for tetracycline controlled gene expression systems in eukaryotic cells. Nucleic Acids Res. 21: 4411-4412.
22. Grall N, et al. 2009. Pivotal role of the Francisella tularensis heat-shock sigma factor RpoH. Microbiology 155: 2560-2572.
23. Guo XV, et al. 2007. Silencing Mycobacterium smegmatis by using tetracycline repressors. J. Bacteriol. 189: 4614-4623.
24. Horzempa J, Carlson PE, Jr, O'Dee DM, Shanks RM, Nau GJ. 2008. Global transcriptional response to mammalian temperature provides new insight into Francisella tularensis pathogenesis. BMC Microbiol. 8: 172. doi: 10.1186/1471-2180-8-172.
25. Horzempa J, et al. 2010. Utilization of an unstable plasmid and the I-SceI endonuclease to generate routine markerless deletion mutants in Francisella tularensis. J. Microbiol. Methods 80: 106-108.
26. Horzempa J, Tarwacki DM, Carlson PE, Jr, Robinson CM, Nau GJ. 2008. Characterization and application of a glucose-repressible promoter in Francisella tularensis. Appl. Environ. Microbiol. 74: 2161-2170.
27. Lathem WW, Price PA, Miller VL, Goldman WE. 2007. A plasminogenactivating protease specifically controls the development of primary pneumonic plague. Science 315: 509-513.
28. LoVullo ED, Molins-Schneekloth CR, Schweizer HP, Pavelka MS, Jr. 2009. Single-copy chromosomal integration systems for Francisella tularensis. Microbiology 155: 1152-1163.
29. LoVullo ED, Sherrill LA, Pavelka MS, Jr. 2009. Improved shuttle vectors for Francisella tularensis genetics. FEMS Microbiol. Lett. 291: 95-102.
30. LoVullo ED, Sherrill LA, Perez LL, Pavelka MS, Jr. 2006. Genetic tools for highly pathogenic Francisella tularensis subsp. tularensis. Microbiology 152: 3425-3435.
31. Margolis JJ, et al. 2010. Contributions of Francisella tularensis subsp. novicida chitinases and Sec secretion system to biofilm formation on chitin. Appl. Environ. Microbiol. 76: 596-608.
32. Meyer TH, et al. 1999. The bacterial SecY/E translocation complex forms channel-like structures similar to those of the eukaryotic Sec61p complex. J. Mol. Biol. 285: 1789-1800.
33. Rigel NW, et al. 2009. The accessory SecA2 system of mycobacteria requires ATP binding and the canonical SecA1. J. Biol. Chem. 284: 9927-9936.
34. Scholz O, et al. 2004. Activity reversal of Tet repressor caused by single amino acid exchanges. Mol. Microbiol. 53: 777-789.
35. Segers K, Anne J. 2011. Traffic jam at the bacterial sec translocase: targeting the SecA nanomotor by small-molecule inhibitors. Chem. Biol. 18: 685-698.
36. Shaner NC, et al. 2008. Improving the photostability of bright monomeric orange and red fluorescent proteins. Nat. Methods 5: 545-551.
37. Starr T, et al. 2012. Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle. Cell Host Microbe 11: 33-45.
38. Tarnok A, Dorger M, Berg I, Gercken G, Schluter T. 2001. Rapid screening of possible cytotoxic effects of particulate air pollutants by measurement of changes in cytoplasmic free calcium, cytosolic pH, and plasma membrane potential in alveolar macrophages by flow cytometry. Cytometry 43: 204-210.
39. Valent QA, et al. 1998. The Escherichia coli SRP and SecB targeting pathways converge at the translocon. EMBO J. 17: 2504-2512.