Cell specific analysis of Arabidopsis leaves using fluorescence activated cell sorting

Journal of Visualized Experiments

Volumn , Issue 68, 2012, Pages

  Grønlund, Jesper T ^a   Eyres, Alison ^a   Kumar, Sanjeev ^a   Buchanan Wollaston, Vicky ^a,b   Gifford, Miriam L ^a,b  

^a UNIVERSITY OF WARWICK   (United Kingdom)

^b UNIVERSITY OF WARWICK   (United Kingdom)

Author keywords

Cell type specificity; Cellular biology; Developmental stage specificity; FACS; Fluorescence activated cell sorting; GFP; Green fluorescent protein; Issue 68; Leaf protoplasts; Molecular biology; Plant biology

Indexed keywords

EID: 84868298337     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/4214     Document Type: Article

References (14)

1. Efroni, I., Eshed, Y., & Lifschitz, E. Morphogenesis of Simple and Compound Leaves: A Critical Review. Plant Cell. 22, 1019-1032, Review., (2010).
2. Kleber, R. & Kehr, J. Preparation and Quality Assessment of RNA From Cell-Specific Samples Obtained by Laser Microdissection. Methods Mol. Biol. 323, 367-377 (2006).
3. Birnbaum, K., Shasha, D.E., Wang, J.Y., Jung, J.W., Lamberts, G.M., Galbraith, D.W., & Benfey, P.N. A Gene Expression Map of the Arabidopsis root. Science. 302, 1956-1960 (2003).
4. Warnasooriya, S.N. & Montgomery, B.L. Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana. J. Vis. Exp. (39), e1925, DOI: 10.3791/1925 (2010).
5. Beal, R.B. & Henikoff, S. A Simple Method For Gene Expression and Chromatin Profiling of Individual Cell Types Within a Tissue. Dev. Cell. 18, 1030-1040 (2010).
6. Mustroph, A., Zanetti, M.E., Jang, C.J.H., Holtan, H.E., Repetti, P.P., Galbraith, D.W., Girke, T., & Bailey-Serres, J. Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis. PNAS. 106, 18843-18848 (2009).
7. Brady S.M., Orlando D.A., Lee J.Y., Wang, J.Y., Koch J., Dinneny J.R., Mace D., Ohler U., & Benfey P.N. Dominant Root Expression Patterns Revealed in a High-Resolution Spatiotemporal Expression Map. Science. 318, 801-806 (2007).
8. Gifford M.L., Dean, A., Gutierrez R.A., Coruzzi G.M., & Birnbaum K.D. Cell-specific nitrogen responses mediate developmental plasticity. PNAS. 105, 803-808 (2008).
9. Dinneny J.R., Long T.A., Wang J.Y., Jung J.W., Mace D. Pointer S., Barron C., Brady S.M., Schiefelbein J., & Benfey P.N. Cell identity mediates the response of Arabidopsis roots to abiotic stress. Science. 320, 942-945 (2008).
10. Peterson, S.V., Johansson, A.I., Kowalczyk, M., Makoveychuk, A., Wang, J.Y., Moritz, T., Grebe, M., Benfey, P.N. Sandberg, G., & Ljung, K. An Auxin Gradient and Maximum in the Arabidopsis Root Apex Shown by High-Resolution Cell-Specific Analysis of IAA Distribution and Synthesis. Plant Cell. 21, 1659-1668 (2009).
11. Yadav, R.K., Girke, T., Pasala, S., Xie, M., & Reddy, G.V. Gene expression map of the Arabidopsis shoot apical meristem stem cell niche. PNAS. 106, 4941-4946 (2009).
12. Harkins, K.R., Jefferson, R.A., Kavanagh, T.A., Bevan, M.W., & Galbraith, D.W. Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts. PNAS. 87, 816-820 (1990).
13. Yang, Y., Costa, A., Leonhardt, N., Siegel, R.S., & Schroeder, J.I. Isolation of a strong Arabidopsis guard cell promoter and it's potential as a research tool. Plant Methods. 4, 6 (2008).
14. Gardner, M.J., Baker, A.J., Assie, J.-M., Poethig, R.S., Haseloff, J.P., & Webb, A.A.R. GAL4 GFP enhancer trap lines for analysis of stomatal guard cell development and gene expression. J. Exp. Bot. 60, 213-226 (2009).