Cell tracking using photoconvertible proteins during zebrafish development

Journal of Visualized Experiments

Volumn , Issue 67, 2012, Pages

  Lombardo, Verónica A ^a   Sporbert, Anje ^a   Abdelilah Seyfried, Salim ^a  

^a Max Delbrück Center for Molecular Medicine   (United Kingdom)

Author keywords

Cell tracking; Cellular biology; Danio rerio; Developmental biology; Embryo; Issue 67; Live cell imaging; Molecular biology; Photoconvertible fluorescent proteins; Tissue morphogenesis; Zebrafish

Indexed keywords

EID: 84868293600     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/4350     Document Type: Article

References (22)

1. Day, R.N. & Davidson, M.W. The fluorescent protein palette: tools for cellular imaging. Chem. Soc. Rev. 38, 2887-2921 (2009).
2. Ando, R., Hama, H., Yamamoto-Hino, M., Mizuno, H., & Miyawaki, A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. U. S. A. 99, 12651-12656 (2002).
3. Tsutsui, H., Karasawa, S., Shimizu, H., Nukina, N., & Miyawaki, A. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6, 233-238 (2005).
4. Gurskaya, N.G., Verkhusha, V.V., Shcheglov, A.S., Staroverov, D.B., Chepurnykh, T.V., Fradkov, A.F., Lukyanov, S., & Lukyanov, K.A. Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nat. Biotechnol. 24, 461-465 (2006).
5. Wiedenmann, J., Ivanchenko, S., Oswald, F., Schmitt, F., Rocker, C., Salih, A., Spindler, K.D., & Nienhaus, G.U. EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. Proc. Natl. Acad. Sci. U.S.A. 101, 15905-15910 (2004).
6. Hatta, K., Tsujii, H., & Omura, T. Cell tracking using a photoconvertible fluorescent protein. Nat. Protoc. 1, 960-967 (2006).
7. Stark, D.A., & Kulesa, P.M. An in vivo comparison of photoactivatable fluorescent proteins in an avian embryo model. Dev. Dyn. 236, 1583-1594 (2007).
8. Kulesa, P.M., Teddy, J.M., Stark, D.A., Smith, S.E., & McLennan, R. Neural crest invasion is a spatially-ordered progression into the head with higher cell proliferation at the migratory front as revealed by the photoactivatable protein, KikGR. Dev. Biol. 316, 275-287 (2008).
9. Nowotschin, S. & Hadjantonakis, A.K. Use of KikGR a photoconvertible green-to-red fluorescent protein for cell labeling and lineage analysis in ES cells and mouse embryos. BMC Dev. Biol. 9, 49 (2009).
10. Griswold, S.L., Sajja, K.C., Jang, C.W., & Behringer, R.R. Generation and characterization of iUBC-KikGR photoconvertible transgenic mice for live time-lapse imaging during development. Genesis. 49, 591-598 (2011).
11. Baker, S.M., Buckheit, R.W., 3rd, & Falk, M.M. Green-to-red photoconvertible fluorescent proteins: tracking cell and protein dynamics on standard wide-field mercury arc-based microscopes. BMC Cell Biol. 11, 15 (2010).
12. Watanabe, S., Punge, A., Hollopeter, G., Willig, K.I., Hobson, R.J., Davis, M.W., Hell, S.W., & Jorgensen, E.M. Protein localization in electron micrographs using fluorescence nanoscopy. Nat. Methods. 8, 80-84 (2011).
13. Habuchi, S., Tsutsui, H., Kochaniak, A.B., Miyawaki, A., & van Oijen, A.M. mKikGR, a monomeric photoswitchable fluorescent protein. PLoS One. 3, e3944 (2008).
14. Fang, Z., Takizawa, N., Wilson, K.A., Smith, T.C., Delprato, A., Davidson, M.W., Lambright, D.G., & Luna, E.J. The membrane-associated protein, supervillin, accelerates F-actin-dependent rapid integrin recycling and cell motility. Traffic. 11, 782-799 (2010).
15. Kimmel, C.B., Ballard, W.W., Kimmel, S.R., Ullmann, B., & Schilling, T.F. Stages of embryonic development of the zebrafish. Dev. Dyn. 203, 253-310 (1995).
16. Isogai, S., Horiguchi, M., & Weinstein, B.M. The vascular anatomy of the developing zebrafish: an atlas of embryonic and early larval development. Dev. Biol. 230, 278-301 (2001).
17. Lazic, S. & Scott, I.C. Mef2cb regulates late myocardial cell addition from a second heart field-like population of progenitors in zebrafish. Dev. Biol. 354, 123-133 (2011).
18. McKinney, S.A., Murphy, C.S., Hazelwood, K.L., Davidson, M.W., & Looger, L.L. A bright and photostable photoconvertible fluorescent protein. Nat. Methods. 6, 131-133 (2009).
19. Godinho, L. Live imaging of zebrafish development. Cold Spring Harb Protoc. 2011, 770-777 (2011).
20. Curran, K., Lister, J.A., Kunkel, G.R., Prendergast, A., Parichy, D.M., & Raible, D.W. Interplay between Foxd3 and Mitf regulates cell fate plasticity in the zebrafish neural crest. Dev. Biol. 344, 107-118 (2010).
21. Nienhaus, G.U., Nienhaus, K., Holzle, A., Ivanchenko, S., Renzi, F., Oswald, F., Wolff, M., Schmitt, F., Rocker, C., Vallone, B., Weidemann, W., Heilker, R., Nar, H., & Wiedenmann, J. Photoconvertible fluorescent protein EosFP: biophysical properties and cell biology applications. Photochem. Photobiol. 82, 351-358 (2006).
22. th ed., Univ. of Oregon Press, Eugene (2000).