Detection of nitric oxide and superoxide radical anion by electron paramagnetic resonance spectroscopy from cells using spin traps

Journal of Visualized Experiments

Volumn , Issue 66, 2012, Pages

  Gopalakrishnan, Bhavani ^a   Nash, Kevin M ^a   Velayutham, Murugesan ^a   Villamena, Frederick A ^a,b  

^a OHIO STATE UNIVERSITY   (United States)

^b THE OHIO STATE UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

Biophysics; Cellular biology; eNOS; EPR; Issue 66; Molecular biology; NO; Physics; ROS; Spin trap; Superoxide

Indexed keywords

NITRIC OXIDE; NITROGEN OXIDE; NITRONES; SUPEROXIDE;

ANIMAL; ARTICLE; CATTLE; CHEMISTRY; ELECTRON SPIN RESONANCE; ENDOTHELIUM CELL; METHODOLOGY; SPIN TRAPPING;

ANIMALS; CATTLE; ELECTRON SPIN RESONANCE SPECTROSCOPY; ENDOTHELIAL CELLS; NITRIC OXIDE; NITROGEN OXIDES; SPIN TRAPPING; SUPEROXIDES;

EID: 84868266756     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2810     Document Type: Article

References (26)

1. Winterbourn, C.C. Reconciling the chemistry and biology of reactive oxygen species. Nat. Chem. Biol. 4, 278-86 (2008).
2. Winterbourn, C.C. & Hampton, M.B. Thiol chemistry and specificity in redox signaling. Free Radic. Biol. Med. 45, 549-61 (2008).
3. Clerch, L.B., Massaro, D.J., & Editors. Oxygen, Gene Expression, and Cellular Function. (1997).
4. Gutteridge, J.M.C. & Halliwell, B. Antioxidants: Molecules, medicines, and myths. Biochem. Biophys. Res. Commun. 393, 561-564 (2010).
5. Sumimoto, H. Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species. FEBS J. 275, 3249-3277 (2008).
6. Ignarro, L.J., Editor Nitric Oxide: Biology and Pathobiology. Academic Press., San Diego, CA, (2009).
7. Moreau, M., et al. Differential effects of alkyl- and arylguanidines on the stability and reactivity of inducible NOS heme-dioxygen complexes. Biochemistry. 45, 3988-3999 (2006).
8. Vasquez-Vivar, J., et al. Superoxide generation by endothelial nitric oxide synthase: the influence of cofactors. Proc. Natl. Acad. Sci. U. S. A. 95, 9220-9225 (1998).
9. Villamena, F.A. & Zweier, J.L. Detection of reactive oxygen and nitrogen species by EPR spin trapping. Antioxid. Redox Signal. 6, 619-29 (2004).
10. Finkelstein, E., Rosen, G.M., & Rauckman, E.J. Spin trapping of superoxide and hydroxyl radical: practical aspects. Arch. Biochem. Biophys. 200, 1-16 (1980).
11. Frejaville, C., et al. 5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO): a new phosphorylated nitrone for the efficient in vitro and in vivo spin trapping of oxygen-centered radicals. J. Chem. Soc., Chem. Commun. 1793-4 (1994).
12. Olive, G., Mercier, A., Le Moigne, F., Rockenbauer, A., & Tordo, P. 2-Ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide: Evaluation of the spin trapping properties. Free Radical. Biol. Med. 28, 403-408 (2000).
13. Villamena, F.A. & Zweier, J.L. Superoxide radical trapping and spin adduct decay of 5-tert-butoxycarbonyl-5-methyl-1-pyrroline N-oxide (BocMPO): kinetics and theoretical analysis. J. Chem. Soc., Perkin Trans. 2, 1340-1344 (2002).
14. Tsuchiya, K., et al. Nitric oxide-forming reactions of the water-soluble nitric oxide spin-trapping agent, MGD. Free Radical Biol. Med. 27, 347-355 (1999).
15. Vanin, A.F., Poltorakov, A.P., Mikoyan, V.D., Kubrina, L.N., & van Faassen, E. Why iron-dithiocarbamates ensure detection of nitric oxide in cells and tissues. Nitric Oxide. 15, 295-311 (2006).
16. Rojas Wahl, R.U. Decomposition mechanism of 3-N-morpholinosydnonimine (SIN-1): A density functional study on intrinsic structures and reactivities. J. Mol. Model. 10, 121-129 (2004).
17. Klempner, M.S. & Gallin, J.I. Separation and functional characterization of human neutrophil subpopulations. Blood. 51, 659-69 (1978).
18. Pou, S., et al. Spin trapping of nitric oxide by ferro-chelates: kinetic and in vivo pharmacokinetic studies. Biochim. Biophys. Acta. 1427, 216-26 (1999).
19. Finkelstein, E., Rosen, G.M., & Rauckman, E.J. Spin trapping. Kinetics of the reaction of superoxide and hydroxyl radicals with nitrones. J. Am. Chem. Soc. 102, 4994-9 (1980).
20. Pou, S., Cohen, M., Britigan, B.E., & Rosen, G. M. Spin-trapping and human neutrophils. Limits of detection of hydroxyl radical. J. Biol. Chem. 264, 12299-12302 (1989).
21. Frejaville, C., et al. 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide: A new efficient phosphorylated nitrone for the in vitro and in vivo spin trapping of oxygen-centered radicals. J. Med. Chem. 38, 258-265 (1995).
22. Snyrychova, I. Improvement of the sensitivity of EPR spin trapping in biological systems by cyclodextrins: A model study with thylakoids and photosystem II particles. Free Radical Biol. Med. 48, 264-274 (2010).
23. Han, Y., et al. Lipophilic beta-cyclodextrin cyclic-nitrone conjugate: Synthesis and spin trapping studies. J. Org. Chem. 74, 5369-5380 (2009).
24. Han, Y., Tuccio, B., Lauricella, R., & Villamena, F.A. Improved spin trapping properties by beta-cyclodextrin-cyclic nitrone conjugate. J. Org. Chem. 73, 7108-7117 (2008).
25. Hardy, M., et al. Detection, characterization, and decay kinetics of ROS and thiyl adducts of mito-DEPMPO spin trap. Chem. Res. Toxicol. 20, 1053-1060 (2007).
26. Kim, S.-U., et al. Fast reactivity of a cyclic nitrone-calix[4]pyrrole conjugate with superoxide radical anion: Theoretical and experimental studies. J. Am. Chem. Soc. 132, 17157-17173 (2010).