Neo-islet formation in liver of diabetic mice by helper-dependent adenoviral vector-mediated gene transfer

Journal of Visualized Experiments

Volumn , Issue 68, 2012, Pages 1-9

  Li, Rongying ^a   Oka, Kazuhiro ^a   Yechoor, Vijay ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Betacellulin; Gene therapy; Genetics; Helper dependent adenoviral vectors; Islet neogenesis; Issue 68; Medicine; Neurogenin3; Physiology; Type 1diabetes

Indexed keywords

EID: 84868266409     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/4321     Document Type: Article

References (10)

1. Parks, R.J., et al. A helper-dependent adenovirus vector system: removal of helper virus by Cre-mediated excision of the viral packaging signal. Proc. Natl. Acad. Sci. U. S. A. 93, 13565-13570 (1996).
2. Kim, I.H., Jozkowicz, A., Piedra, P.A., Oka, K., & Chan, L. Lifetime correction of genetic deficiency in mice with a single injection of helperdependent adenoviral vector. Proc. Natl. Acad. Sci. U.S.A. 98, 13282-13287 (2001).
3. Belalcazar, L.M., et al. Long-term stable expression of human apolipoprotein A-I mediated by helper-dependent adenovirus gene transfer inhibits atherosclerosis progression and remodels atherosclerotic plaques in a mouse model of familial hypercholesterolemia. Circulation. 107, 2726-2732 (2003).
4. Oka, K., et al. Long-term stable correction of low-density lipoprotein receptor-deficient mice with a helper-dependent adenoviral vector expressing the very low-density lipoprotein receptor. Circulation. 103, 1274-1281 (2001).
5. Nomura, S., et al. Low-density lipoprotein receptor gene therapy using helper-dependent adenovirus produces long-term protection against atherosclerosis in a mouse model of familial hypercholesterolemia. Gene Ther. 11, 1540-1548 (2004).
6. Ng, P., et al. A high-efficiency Cre/loxP-based system for construction of adenoviral vectors. Hum. Gene Ther. 10, 2667-2672 (1999).
7. Palmer, D. & Ng, P. Improved system for helper-dependent adenoviral vector production. Mol. Ther. 8, 846-852 (2003).
8. Oka, K. & Chan, L. Helper-Dependent Adenoviral Vectors In: Current Protocols in Molecular Biology. 16.24.1-16.24.23 (2005).
9. Yechoor, V., et al. Neurogenin3 is sufficient for transdetermination of hepatic progenitor cells into neo-islets in vivo but not transdifferentiation of hepatocytes. Dev. Cell. 16, 358-373 (2009).
10. Yechoor, V., et al. Gene Therapy with Neurogenin 3 and Betacellulin Reverses Major Metabolic Problems in Insulin-Deficient Diabetic Mice. Endocrinology., (2009).