Characterization of methicillin-susceptible and -resistant staphylococci in the clinical setting: A multicentre study in Nigeria

BMC Infectious Diseases

Volumn 12, Issue , 2012, Pages

  Shittu, Adebayo ^a,b   Oyedara, Omotayo ^a,c   Abegunrin, Fadekemi ^a   Okon, Kenneth ^d   Raji, Adeola ^e   Taiwo, Samuel ^f   Ogunsola, Folasade ^g   Onyedibe, Kenneth ^h   Elisha, Gay ^b  

^a OBAFEMI AWOLOWO UNIVERSITY   (Nigeria)

^b UNIVERSITY OF CAPE TOWN   (South Africa)

^c OSUN STATE UNIVERSITY   (Nigeria)

^d UNIVERSITY OF MAIDUGURI   (Nigeria)

^e LAGOS STATE UNIVERSITY TEACHING HOSPITAL   (Nigeria)

^f LADOKE AKINTOLA UNIVERSITY OF TECHNOLOGY   (Nigeria)

^g UNIVERSITY OF LAGOS   (Nigeria)

^h UNIVERSITY OF JOS   (Nigeria)

Author keywords

Coagulase negative staphylococci; mecA gene; Multiresistance; Nigeria; Panton Valentine Leukocidin; SCCmec typing; ST152; Staphylococcus aureus; Staphylococcus haemolyticus; Staphylococcus sciuri

Indexed keywords

CIPROFLOXACIN; CLINDAMYCIN; COTRIMOXAZOLE; ERYTHROMYCIN; FUSIDIC ACID; GENTAMICIN; LINEZOLID; MOXIFLOXACIN; OXACILLIN; PANTON VALENTINE LEUKOCIDIN; PENICILLIN BINDING PROTEIN 2A; PENICILLIN G; PSEUDOMONIC ACID; RIFAMPICIN; TEICOPLANIN; TETRACYCLINE; TIGECYCLINE; VANCOMYCIN;

ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM EXAMINATION; BACTERIUM ISOLATE; CLONE; COAGULASE NEGATIVE STAPHYLOCOCCUS; DRFA GENE; MECA GENE; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; METHICILLIN SUSCEPTIBLE STAPHYLOCOCCUS AUREUS; MULTILOCUS SEQUENCE TYPING; NIGERIA; NONHUMAN; PANTON VALENTINE LEUKOCIDIN GENE; POLYMERASE CHAIN REACTION; SCCMEC TYPING; SPA TYPING; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS HAEMOLYTICUS; STAPHYLOCOCCUS SCIURI;

ANTI-BACTERIAL AGENTS; BACTERIAL TYPING TECHNIQUES; HOSPITALS; HUMANS; METHICILLIN RESISTANCE; MICROBIAL SENSITIVITY TESTS; MOLECULAR EPIDEMIOLOGY; MOLECULAR TYPING; NIGERIA; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS;

EID: 84868248541     PISSN: None     EISSN: 14712334     Source Type: Journal    
DOI: 10.1186/1471-2334-12-286     Document Type: Article

References (52)

1. Chambers HF, Deleo FR. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nat Rev Microbiol 2009, 7(9):629-641. 10.1038/nrmicro2200, 2871281, 19680247.
2. Nickerson EK, West TE, Day NP, Peacock SJ. Staphylococcus aureus disease and drug resistance in resource-limited countries in South and East Asia. Lancet Infect Dis 2009, 9(2):130-135. 10.1016/S1473-3099(09)70022-2, 19179228.
3. Fry DE, Barie PS. The changing face of Staphylococcus aureus: a continuing surgical challenge. Surg Infect (Larchmt) 2011, 12(3):191-203.
4. Huebner J, Goldmann DA. Coagulase-negative staphylococci: role as pathogens. Ann Rev Med 1999, 50:223-236. 10.1146/annurev.med.50.1.223, 10073274.
5. O'Gara JP, Humpreys H. Staphylococcus epidermidis biofilms: importance and implications. J Med Microbiol 2001, 50(7):582-587.
6. Deepa S, Kumari A, Venkatesha D. Increasing trends of methicillin resistant coagulase negative Staphylococcus in neonatal septicaemia - a study in a tertiary care hospital, Mysore, South India. OJHAS 2010, 9(4):1-3.
7. Zong Z, Peng C, Lu X. Diversity of SCCmec elements in methicillin-resistant coagulase-negative staphylococci clinical isolates. PLoS One 2011, 6(5):e20191. 10.1371/journal.pone.0020191, 3102682, 21637845.
8. Venkatesh MP, Placencia F, Weisman LE. Coagulase negative staphylococcal infections in the neonate and child: an update. Semin Pediatr Infect Dis 2006, 17(3):120-127. 10.1053/j.spid.2006.06.005, 16934706.
9. Anderson-Berry A, Brinton B, Lyden E, Faix RG. Risk factors associated with development of persistent coagulase negative staphylococci bacteremia in the neonate and associated short-term and discharge morbidities. Neonatology 2011, 99(1):23-31. 10.1159/000292567, 20588067.
10. Bassetti M, Trecharichi EM, Mesini A, Spanu T, Giacobbe DR, Rossi M, Shenone E, Pascale GD, Molinari MP, Cauda R, Viscoli C, Tumbarello M. Risk factors and mortality of healthcare-associated and community-acquired Staphylococcus aureus bacteraemia. Clin Microbiol Infect 2011, Epub ahead of print.
11. de Kraker ME, Davey PG, Grundmann H, . on behalf of the BURDEN study group Mortality and hospital stay associated with resistant Staphylococcus aureus and Escherichia coli bacteremia: estimating the burden of antibiotic resistance in Europe. PLoS Med 2011, 8(10):e1001104. 10.1371/journal.pmed.1001104, 3191157, 22022233, on behalf of the BURDEN study group.
12. Jain A, Agarwal A, Verma RK, Awasthi S, Singh KP. Intravenous device associated blood stream staphylococcal infection in paediatric patients. Indian J Med Res 2011, 134(2):193-199. 3181020, 21911972.
13. Jean-Baptiste N, Benjamin DK, Cohen-Wolkowiez M, Fowler VG, Laughon M, Clark RH Smith PB. Coagulase negative staphylococcal infections in the neonatal intensive care unit. Infect Control Hosp Epidemiol 2011, 32(7):679-686. 10.1086/660361, 3238054, 21666399.
14. Marra AR, Camargo LF, Pignatari AC, Sukiennik T, Behar PR, Medeiros EA, Ribeiro J, Girão E, Correa L, Guerra C, Brites C, Pereira CA, Carneiro I, Reis M, de Souza MA, Tranchesi R, Barata CU, Edmond MB, . Brazilian SCOPE Study Group Nosocomial bloodstream infections in Brazilian hospitals: analysis of 2,563 cases from a prospective nationwide surveillance study. J Clin Microbiol 2011, 49(5):1866-1871. 10.1128/JCM.00376-11, 3122653, 21411591, Brazilian SCOPE Study Group.
15. Akinkunmi EO, Lamikanra A. Species distribution and antibiotic resistance in coagulase negative staphylococci colonizing the gastrointestinal tract of children in Ile-Ife, Nigeria. Trop J Pharm Res 2010, 9(1):35-43.
16. Akinjogunla OJ, Enabulele IO. Virulence factors, plasmid profiling and curing analysis of multidrug resistant Staphylococcus aureus and coagulase negative Staphylococcus spp. isolated from patients with acute otitis media. J American Sci 2010, 6(11):1022-1033.
17. Efuntoye MO. Study of antibiotic sensitivity pattern and enterotoxigenicity of staphylococci isolated from swimming pools in Ibadan, Nigeria. World Appl Sci J 2010, 9(11):1324-1327.
18. Jombo GTA, Akpan S, Epoke J, Akaa PD, Eyong KI, Gyuse AN, Okwori EE, Etukumana EA. Antimicrobial susceptibility profile of community acquired and nosocomial isolates of Staphylococcus aureus and that of coagulase negative staphylococci from clinical blood culture specimens at a Nigerian University Teaching Hospital. J Clin Med Res 2010, 2(6):83-90.
19. Shittu AO, Lin J, Morrison D, Kolawole DO. Isolation and molecular characterization of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus associated with skin and soft-tissue infections. J Med Microbiol 2004, 53(Pt1):51-55.
20. Shittu AO, Lin J, Morrison D, Kolawole DO. Identification and molecular characterization of mannitol positive, coagulase negative staphylococci from nasal samples of medical personnel and students. J Med Microbiol 2006, 55(Pt3):317-324.
21. Ghebremedhin B, Olugbosi MO, Raji AM, Layer F, Bakare RA, König B, König W. Emergence of a community-associated methicillin-resistant Staphylococcus aureus strain with a unique resistance profile in Southwest Nigeria. J Clin Microbiol 2009, 47(9):2975-2980. 10.1128/JCM.00648-09, 2738091, 19571020.
22. Okon KO, Basset P, Uba A, Lin J, Shittu AO, Blanc DS. Co-occurrence of predominant Panton-Valentine leukocidin-positive sequence type (ST) 152 and multi-drug resistant ST241 Staphylococcus aureus clones in Nigerian hospitals. J Clin Microbiol 2009, 47(9):3000-3003. 10.1128/JCM.01119-09, 2738054, 19605575.
23. Shittu AO, Okon K, Adesida S, Oyedara O, Witte W, Strommenger B, Layer F, Nübel U. Antibiotic resistance and molecular epidemiology of Staphylococcus aureus in Nigeria. BMC Microbiol 2011, 11:92. 10.1186/1471-2180-11-92, 3112067, 21545717.
24. Martineau F, Picard FJ, Ke D, Paradis S, Roy PH, Ouellette M, Bergeron MG. Development of a PCR assay for identification of staphylococci at genus and species levels. J Clin Microbiol 2001, 39(7):2541-2547. 10.1128/JCM.39.7.2541-2547.2001, 88182, 11427566.
25. Ender M, Berger-Bächi B, McCallum N. Variability in SCCmecN1 spreading among injection drug users in Zurich, Switzerland. BMC Microbiol 2007, 7:62. 10.1186/1471-2180-7-62, 3225876, 17605795.
26. Milheiriço C, Oliveira DC, de Lencastre H. Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus. Antimicrob Agents Chemother 2007, 51(9):3374-3377. Epub 2007 Jun 18. Erratum in: Antimicrob Agents Chemother 2007, 51(12):4537, 10.1128/AAC.00275-07, 2043198, 17576837.
27. Kondo Y, Ito T, Ma XX, Watanabe S, Kreiswirth BN, Etienne J, Hiramatsu K. Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec, ccr and major differences in junkyard regions. Antimicrob Agents Chemother 2007, 51(1):264-274. 10.1128/AAC.00165-06, 1797693, 17043114.
28. International Working Group on the classification of Staphylococcal Cassette Chromosome elements (IWG-SCC) Classification of staphylococcal cassette chromosome mec (SCCmec): guidelines for reporting novel SCCmec elements. Antimicrob Agents Chemother 2009, 53(12):4961-4967. 2786320, 19721075, International Working Group on the classification of Staphylococcal Cassette Chromosome elements (IWG-SCC).
29. Lina G, Piémont Y, Godail-Gamot F, Bes M, Peter MO, Gauduchon V, Vandenesch F, Etienne J. Involvement of Panton-Valentine Leukocidin-Producing Staphylococcus aureus in Primary Skin Infections and Pneumonia. Clin Infect Dis 1999, 29(5):1128-1132. 10.1086/313461, 10524952.
30. Harmsen D, Claus H, Witte W, Rothgänger J, Claus H, Turnwald D, Vogel U. Typing of methicillin-resistant Staphylococcus aureus in a university setting by using novel software for spa repeat determination and database management. J Clin Microbiol 2003, 41(12):5442-5448. 10.1128/JCM.41.12.5442-5448.2003, 309029, 14662923.
31. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 2000, 38(3):1008-1015. 86325, 10698988.
32. Ing MB, Baddour LM, Bayer AS. Bacteremia and infective endocarditis: pathogenesis, diagnosis, and complications. The Staphylococci In Human Disease 1997, 331-354. New York: Churchill Livingstone, Crossley KB, Archer GL.
33. Bouchami O, Ben Hassen A, de Lencastre H, Miragaia M. High prevalence ofmeccomplex C andccrCis independent of SCCmectype V inStaphylococcus haemolyticus. Eur J Clin Microbiol Infect Dis 2012, 31(4):605-614. 10.1007/s10096-011-1354-3, 21822565.
34. Wallet F, Stuit L, Boulanger E, Roussel-Delvallez M, Dequiedt P, Courcol RJ. Peritonitis due to Staphylococcus sciuri in a patient on continuous ambulatory peritoneal dialysis. Scand J Infect Dis 2000, 32(6):697-698. 10.1080/003655400459667, 11200385.
35. Stepanovic S, Jezek P, Vukovic D, Dakic I, Petras P. Isolation of members of the Staphylococcus sciuri group from urine and their relationship to urinary tract infections. J Clin Microbiol 2003, 41(11):5262-5264. 10.1128/JCM.41.11.5262-5264.2003, 262515, 14605178.
36. Coimbra DG, Almeida AGCS, Júnior JBO, da Silva AF, Pimentel BJ, Gitaí DLG, Moreira LS, Silva-Filho EA, de Andrade TG. Wound infection by multiresistant Staphylococcus sciuri identified by molecular methods. New Microbiol 2011, 34(4):425-427.
37. Shittu AO, Lin J, Kolawole DO. Antimicrobial susceptibility patterns of Staphylococcus aureus and characterization of MRSA in Southwestern Nigeria. WOUNDS 2006, 18(4):77-84.
38. Garza-González E, Morfin-Otero R, Llaca-Diaz JM, Rodriguez-Noriega E. Staphylococcal cassette chromosome (SCCmec) in methicillin-resistant coagulase negative staphylococci. A review and the experience in a tertiary-care setting. Epidemiol Infect 2010, 138(5):645-654. 10.1017/S0950268809991361, 19961645.
39. Hanssen AM, Ericson Sollid JU. SCCmec in staphylococci: genes on the move. FEMS Immunol Med Microbiol 2006, 46(1):8-20. 10.1111/j.1574-695X.2005.00009.x, 16420592.
40. Ito T, Katayama Y, Hiramatsu K. Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315. Antimicrob Agents Chemother 1999, 43(6):1449-1458. 89295, 10348769.
41. Ito T, Katayama Y, Asada K, Mori N, Tsutsumimoto K, Tiensasitorn C, Hiramatsu K. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2001, 45(5):1323-1336. 10.1128/AAC.45.5.1323-1336.2001, 90469, 11302791.
42. Ma XX, Ito T, Tiensasitorn C, Jamklang M, Chongtrakool P, Boyle-Vavra S, Daum RS, Hiramatsu K. Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob Agents Chemother 2002, 46(4):1147-1152. 10.1128/AAC.46.4.1147-1152.2002, 127097, 11897611.
43. Ito T, Ma XX, Takeuchi F, Okuma K, Yuzawa H, Hiramatsu K. Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC. Antimicrob Agents Chemother 2004, 48(7):2637-2651. 10.1128/AAC.48.7.2637-2651.2004, 434217, 15215121.
44. Oliveira DC, Milheiriço C, de Lencastre H. Redefining a structural variant of staphylococcal cassette chromosome mec, SCCmec type VI. Antimicrob Agents Chemother 2006, 50(10):3457-3459. 10.1128/AAC.00629-06, 1610060, 17005831.
45. Berglund C, Ito T, Ikeda M, Ma XX, Söderquist B, Hiramatsu K. Novel type of staphylococcal cassette chromosome mec in a methicillin-resistant Staphylococcus aureus strain isolated in Sweden. Antimicrob Agents Chemother 2008, 52(10):3512-3516. 10.1128/AAC.00087-08, 2565894, 18676883.
46. Zhang K, McClure JA, Elsayed S, Conly JM. Novel staphylococcal cassette chromosome mec type, tentatively designated type VIII, harboring class A mec and type 4 ccr gene complexes in a Canadian epidemic strain of methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2009, 53(2):531-540. 10.1128/AAC.01118-08, 2630601, 19064897.
47. Li S, Skov RL, Han X, Larsen AR, Larsen J, Sørum M, Wulf M, Voss A, Hiramatsu K, Ito T. Novel types of staphylococcal cassette chromosome mec elements identified in clonal complex 398 methicillin resistant Staphylococcus aureus strains. Antimicrob Agents Chemother 2011, 55(6):3046-3050. 10.1128/AAC.01475-10, 3101438, 21422209.
48. Shore AC, Deasy EC, Slickers P, Brennan G, O'Connell B, Monecke S, Ehricht R, Coleman DC. Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2011, 55(8):3765-3773. 10.1128/AAC.00187-11, 3147645, 21636525.
49. Barros EM, Ceotto H, Bastos MCF, dos Santos KRN, Giambiagi-deMarval M. Staphylococcus haemolyticus as an important hospital pathogen and carrier of methicillin resistance genes. J Clin Microbiol 2012, 50(1):166-168. 10.1128/JCM.05563-11, 3256717, 21976766.
50. Ruimy R, Maiga A, Armand-Lefevre L, Maiga I, Diallo A, Koumaré AK, Ouattara K, Soumaré S, Gaillard K, Lucet JC, Andremont A, Feil EJ. The carriage population of Staphylococcus aureus from Mali is composed of a combination of pandemic clones and the divergent Panton-Valentine leukocidin-positive genotype ST152. J Bacteriol 2008, 190(11):3962-3968. 10.1128/JB.01947-07, 2395057, 18375551.
51. Breurec S, Fall C, Pouillot R, Boisier P, Brisse S, Diene-Sarr F, Djibo S, Etienne J, Fonkoua MC, Perrier-Gros-Claude JD, Ramarokoto CE, Randrianirina F, Thiberge JM, Zriouil SB, Garin B, Laurent F, . Working Group on Staphylococcus aureus Infections Epidemiology of methicillin-susceptible Staphylococcus aureus lineages in five major African towns: high prevalence of Panton-Valentine leukocidin genes. Clin Microbiol Infect 2010, 17(4):633-639. Working Group on Staphylococcus aureus Infections.
52. Schaumburg F, Ngoa AU, Kösters K, Köck R, Adegnika AA, Kremsner PG, Lell B, Peters G, Mellmann A, Becker K. Virulence and genotypes of Staphylococcus aureus from infection and carriage in Gabon. Clin Microbiol Infect 2011, 17(10):1507-1513. 10.1111/j.1469-0691.2011.03534.x, 21595798.