Purification of urease from jack bean (Canavalia ensiformis) with copper (II) chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(l)-histidine methyl ester) cryogels

Journal of Molecular Recognition

Volumn 25, Issue 11, 2012, Pages 549-554

  Tekiner, Pinar ^a   Perçin, Işik ^a   Ergün, Bahar ^a   Yavuz, Handan ^a   Aksöz, Erol ^a  

^a HACETTEPE UNIVERSITY   (Turkey)

Author keywords

Canavalia ensiformis; cryogels; IMAC; jack bean urease (JBU); PHEMAH

Indexed keywords

BUFFER; CHELATING AGENT; COPPER; DRUG DERIVATIVE; HISTIDINE; POLY(HYDROXYETHYL METHACRYLATE N METHACRYLOYL HISTIDINE METHYL ESTER); POLY(HYDROXYETHYL METHACRYLATE-N-METHACRYLOYL-HISTIDINE METHYL ESTER); POLYMETHACRYLIC ACID DERIVATIVE; UREASE;

AFFINITY CHROMATOGRAPHY; ATOMIC ABSORPTION SPECTROMETRY; CANAVALIA; CHEMISTRY; CONFERENCE PAPER; CRYOGEL; ISOLATION AND PURIFICATION; KINETICS; METHODOLOGY; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; SCANNING ELECTRON MICROSCOPY;

BUFFERS; CANAVALIA; CHELATING AGENTS; CHROMATOGRAPHY, AFFINITY; COPPER; CRYOGELS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HISTIDINE; HYDROGEN-ION CONCENTRATION; KINETICS; MICROSCOPY, ELECTRON, SCANNING; MOLECULAR WEIGHT; POLYMETHACRYLIC ACIDS; SPECTROPHOTOMETRY, ATOMIC; UREASE;

CANAVALIA ENSIFORMIS;

EID: 84868123230     PISSN: 09523499     EISSN: 10991352     Source Type: Journal    
DOI: 10.1002/jmr.2204     Document Type: Conference Paper

References (27)

1. Alkan H, Bereli N, Baysal Z, Denizli A,. 2010. Selective removal of the autoantibodies from rheumatoid arthritis patient plasma using protein A carrying affinity cryogels. Biochem. Eng. J. 51: 153-159.
2. Allen SJ, Koumanova B, Kircheva Z, Nenkova S,. 2005. Binding of 2-nitrophenol by technical hydrolysis lignin: Kinetics, mass transfer, and equilibrium studies. Ind Eng Chem Res. 44: 2281-2287.
3. Arnold FH,. 1991. Metal-affinity separations: a new dimension in protein processing. Biotechnology (N. Y). 9: 151-156.
4. Arvidsson P, Plieva FM, Lozinsky VI, Galaev IY, Mattiasson B,. 2003. Direct chromatographic capture of enzyme from crude homogenate using immobilized metal affinity chromatography on a continuous supermacroporous adsorbent. J. Chromatogr. A 986: 275-290.
5. Balasubramanian A, Ponnuraj K,. 2009. Purification, crystallization and preliminary X-ray analysis of urease from jack bean (Canavalia ensiformis). Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. F65: 949-951.
6. Bankapalli LK, Singh AN, Patra S, Dubey VK,. 2011. Purification, characterization and immobilization of urease from Momordica charantia seeds. Process Biochem. 46: 1486-1491.
7. Bereli N, Andac M, Baydemir G, Say R, Galaev IY, Denizli A,. 2008. Protein recognition via ion-coordinated molecularly imprinted supermacroporous cryogels. J. Chromatogr. A 1190: 18-26.
8. Bereli N, Sener G, Yavuz H, Denizli A,. 2011. Oriented immobilized anti-LDL antibody carrying poly(hydroxyethyl methacrylate) cryogel for cholesterol removal from human plasma. Mat Sci Eng C. 31: 1078-1083.
9. Boyer PD,. 1970. The Enzymes. Academic Press: New York.
10. Bradford MM,. 1976. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72: 248-254.
11. Chaga GS,. 2001. Twenty-five years of immobilized metal ion affinity chromatography: past, present and future. J. Biochem. Biophys. Chromatogr. 49: 313-334.
12. Cheung CW, Porter JF, Mckay G,. 2001. Sorption kinetic analysis for the removal of cadmium ions from effluents using bone char. Water Res. 35: 605-612.
13. Garberc-Porekar V, Menart V,. 2001. Perspectives of immobilized-metal affinity chromatography. J. Biochem. Biophys. Meth. 49: 335-360.
14. Garipcan B, Denizli A,. 2002. A novel affinity support material for the separation of immunoglobulin G from human plasma. Macromol. Biosci. 2: 135-144.
15. Jabri J, Lee MH, Hausinger RP, Karplus PA,. 1992. Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes. J. Mol. Biol. 227: 934-937.
16. Laemmli UK,. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
17. Lozinsky VI, Galaev IY, Plieva FM, Savina IN, Jungvid H, Mattiasson B,. 2003. Polymeric cryogels as promising materials of biotechnological interest. Trends Biotechnol. 21: 445-451.
18. Mendes MJ, Karmali A, Brown P,. 1988. One-step affinity purification of urease from jack beans. Biochimie 70: 1369-1371.
19. Öncel S, Uzun L, Garipcan B, Denizli A,. 2005. Synthesis of phenylalanine-containing hydrophobic beads for lysozyme binding. Ind Eng Chem Res. 44: 7049-7056.
20. Porath J, Carlsson J, Olsson I, Belfrage G,. 1975. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature, 258: 598-599.
21. Sumner JB,. 1926. The isolation and crystallization of the enzyme urease: preliminary paper. J. Biol. Chem. 69: 435-441.
22. Sumner JB, Hand DB,. 1928. Crystalline Urease II. J. Biol. Chem. 76: 149-162.
23. Sumner JB, Myrback K,. 1951. The Enzymes. Merck and Co. Inc.: New Jersey.
24. Vial S, Prevot V, Leroux F, Forano C,. 2008. Immobilization of urease in ZnAl Layered Double Hydroxides by soft chemistry routes. Microporous Mesoporous Mater. 107: 190-201.
25. 4, and NaCl as precipitants. Cryst. Growth Des. 8: 711-716.
26. Weber M, Jones J, Ulrich, J,. 2008. Optimisation of isolation and purification of the jack bean enzyme urease by extraction and subsequent crystallization. Food Bioprod. Process. 86: 43-52.
27. Yilmaz F, Bereli N, Yavuz H, Denizli A,. 2009. Supermacroporous hydrophobic affinity cryogels for protein chromatography. Biochem. Eng. J. 43: 272-279.