Crystal Structure Determination and Mutagenesis Analysis of the Ene Reductase NCR

ChemBioChem

Volumn 13, Issue 16, 2012, Pages 2400-2407

  Reich, Sabrina ^a   Hoeffken, Hans Wolfgang ^b   Rosche, Bettina ^c   Nestl, Bettina M ^a   Hauer, Bernhard ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

^b BASF SE   (Germany)

^c UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

Crystal structures; Enzyme models; Ketones; Loop swapping; Multisequence alignment; Mutagenesis

Indexed keywords

ACETIC ACID; ALKENE; ENE NICOTINAMIDE DEPENDENT CYCLOHEX 2 EN 1 ONE REDUCTASE; FLAVINE MONONUCLEOTIDE; NICOTINAMIDE; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE DEHYDROGENASE; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENANTIOSELECTIVITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBSTRATE; GENETIC VARIABILITY; LOOP DIRECTED MUTAGENESIS; MUTATIONAL ANALYSIS; NONHUMAN; PRIORITY JOURNAL; REDUCTION; SACCHAROMYCES; SACCHAROMYCES PASTORIANUS; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; WILD TYPE;

CRYSTALLOGRAPHY, X-RAY; MODELS, MOLECULAR; MOLECULAR STRUCTURE; MUTAGENESIS; OXIDOREDUCTASES; PROTEIN CONFORMATION; ZYMOMONAS;

SACCHAROMYCES PASTORIANUS; ZYMOMONAS MOBILIS;

EID: 84868087352     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.201200404     Document Type: Article

References (29)

1. R. M. Kohli, V. Massey, J. Biol. Chem. 1998, 273, 32763-32770; B. J. Brown, J.-W. Hyun, S. Duvvuri, P. A. Karplus, V. Massey, J. Biol. Chem. 2002, 277, 2138-2145; D. Xu, R. M. Kohli, V. Massey, Proc. Natl. Acad. Sci. USA 1999, 96, 3556-3561.
2. K. Kitzing, T. B. Fitzpatrick, C. Wilken, J. Sawa, G. P. Bourenkov, P. Macheroux, T. Clausen, J. Biol. Chem. 2005, 280, 27904-27913.
3. H. S. Toogood, J. M. Gardiner, N. S. Scrutton, ChemCatChem 2010, 2, 892-914.
4. R. Stuermer, B. Hauer, M. Hall, K. Faber, Curr. Opin. Chem. Biol. 2007, 11, 203-213.
5. M. L. Hall, A. S. Bommarius, Chem. Rev. 2011, 111, 4088-4110.
6. K. M. Fox, P. A. Karplus, Structure 1994, 2, 1089-1105; T. M. Barna, H. Khan, N. C. Bruce, I. Barsukov, N. S. Scrutton, P. C. E. Moody, J. Mol. Biol. 2001, 310, 433-447.
7. A. Fryszkowska, H. Toogood, M. Sakuma, J. M. Gardiner, G. M. Stephens, N. S. Scrutton, Adv. Synth. Catal. 2009, 351, 2976-2990; M. Hall, C. Stueckler, H. Ehammer, E. Pointner, G. Oberdorfer, K. Gruber, B. Hauer, R. Stuermer, W. Kroutil, P. Macheroux, K. Faber, Adv. Synth. Catal. 2008, 350, 411-418.
8. M. Hall, C. Stueckler, B. Hauer, R. Stuermer, T. Friedrich, M. Breuer, W. Kroutil, K. Faber, Eur. J. Org. Chem. 2008, 1511-1516.
9. M. Hall, C. Stueckler, W. Kroutil, P. Macheroux, K. Faber, Angew. Chem. 2007, 119, 4008-4011; Angew. Chem. Int. Ed. 2007, 46, 3934-3937.
10. N. J. Mueller, C. Stueckler, B. Hauer, N. Baudendistel, H. Housden, N. C. Bruce, K. Faber, Adv. Synth. Catal. 2010, 352, 387-394; C. Stueckler, N. J. Mueller, C. K. Winkler, S. M. Glueck, K. Gruber, G. Steinkellner, K. Faber, Dalton Trans. 2010, 39, 8472-8476; R. E. Williams, N. C. Bruce, Microbiology 2002, 148, 1607-1614; A. Mueller, R. Stuermer, B. Hauer, B. Rosche, Angew. Chem. 2007, 119, 3380-3382; Angew. Chem. Int. Ed. 2007, 46, 3316-3318.
11. A. Mueller, B. Hauer, B. Rosche, Biotechnol. Bioeng. 2007, 98, 22-29.
12. O. Warburg, W. Christian, Naturwissenschaften 1932, 20, 980-981.
13. A. Mueller, B. Hauer, B. Rosche, J. Mol. Catal. B 2006, 38, 126-130.
14. F. H. Arnold, Acc. Chem. Res. 1998, 31, 125-131; O. Kuchner, F. H. Arnold, Trends Biotechnol. 1997, 15, 523-530; W. P. C. Stemmer, Nature 1994, 370, 389-391.
15. S. Bartsch, R. Kourist, U. T. Bornscheuer, Angew. Chem. 2008, 120, 1531-1534; Angew. Chem. Int. Ed. 2008, 47, 1508-1511.
16. D. J. Bougioukou, S. Kille, A. Taglieber, M. T. Reetz, Adv. Synth. Catal. 2009, 351, 3287-3305.
17. M. Hoehne, S. Schaetzle, H. Jochens, K. Robins, U. T. Bornscheuer, Nat. Chem. Biol. 2010, 6, 807-813.
18. S. K. Padhi, D. J. Bougioukou, J. D. Stewart, J. Am. Chem. Soc. 2009, 131, 3271-3280.
19. G. Oberdorfer, G. Steinkellner, C. Stueckler, K. Faber, K. Gruber, ChemCatChem 2011, 3, 1562-1566.
20. Z. Prokop, Y. Sato, J. Brezovsky, T. Mozga, R. Chaloupkova, T. Koudelakova, P. Jerabek, V. Stepankova, R. Natsume, J. G. E. van Leeuwen, D. B. Janssen, J. Florian, Y. Nagata, T. Senda, J. Damborsky, Angew. Chem. 2010, 122, 6247-6251; Angew. Chem. Int. Ed. 2010, 49, 6111-6115.
21. P. A. Karplus, K. M. Fox, V. Massey, FASEB J. 1995, 9, 1518-1526.
22. C. Breithaupt, R. Kurzbauer, F. Schaller, A. Stintzi, A. Schaller, R. Huber, P. Macheroux, T. Clausen, J. Mol. Biol. 2009, 392, 1266-1277.
23. A. Brigé, D. van den Hemel, W. Carpentier, L. De Smet, J. J. Van Beeumen, Biochem. J. 2006, 394, 335-344.
24. T. Barna, H. L. Messiha, C. Petosa, N. C. Bruce, N. S. Scrutton, P. C. E. Moody, J. Biol. Chem. 2002, 277, 30976-30983.
25. H. G. W. Leuenberger, W. Boguth, E. Widmer, R. Zell, Helv. Chim. Acta 1976, 59, 1832-1849.
26. M. Hall, B. Hauer, R. Stuermer, W. Kroutil, K. Faber, Tetrahedron: Asymmetry 2006, 17, 3058-3062.
27. W. Kabsch, Acta Crystallogr. Sect. D Biol. Crystallogr. 2010, 66, 125-132.
28. Collaborative Computational Project, Acta Crystallogr. Sect. D Biol. Crystallogr. 1994, 50, 760-763.
29. G. N. Murshudov, P. Skubák, A. A. Lebedev, N. S. Pannu, R. A. Steiner, R. A. Nicholls, M. D. Winn, F. Long, A. A. Vagin, Acta Crystallogr. Sect. D Biol. Crystallogr. 2011, 67, 355-367.