Expression, purification and characterization of a human single-chain Fv antibody fragment fused with the Fc of an IgG1 targeting a rabies antigen in Pichia pastoris

Protein Expression and Purification

Volumn 86, Issue 1, 2012, Pages 75-81

  Wang, Ding Ding ^a   Su, Man Man ^b   Sun, Yan ^a   Huang, Shu Lin ^a   Wang, Ju ^c   Yan, Wei Qun ^b  

^a GUANGDONG PHARMACEUTICAL UNIVERSITY   (China)

^b JILIN UNIVERSITY   (China)

^c National Engineering Research Centre of Genetic Medicine   (China)

Author keywords

Expression; Pichia pastoris; Purification; Rabies; ScFv Fc

Indexed keywords

HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G; NEUTRALIZING ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; VIRUS ANTIBODY; VIRUS ANTIGEN;

ANIMAL; ARTICLE; GENE VECTOR; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; LD 50; METABOLISM; MOUSE; PICHIA; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; RABIES VIRUS; SERODIAGNOSIS; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, NEUTRALIZING; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENETIC VECTORS; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; LETHAL DOSE 50; MICE; NEUTRALIZATION TESTS; PICHIA; PLASMIDS; RABIES VIRUS; RECOMBINANT FUSION PROTEINS; SINGLE-CHAIN ANTIBODIES;

EID: 84867851159     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.08.015     Document Type: Article

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