Automated identification and susceptibility determination directly from blood cultures facilitates early targeted antibiotic therapy

Scandinavian Journal of Infectious Diseases

Volumn 44, Issue 11, 2012, Pages 860-865

  Nilsen, Einar ^a,b  

^a Molde Hospital   (Norway)

^b UNIVERSITY HOSPITAL OF NORTH NORWAY   (Norway)

Author keywords

Antimicrobial therapy; Infectious disease; Microbiology

Indexed keywords

ACCURACY; ANTIBIOTIC SENSITIVITY; ANTIBIOTIC THERAPY; ARTICLE; AUTOMATION; BLOOD CULTURE; CONTROLLED STUDY; GRAM NEGATIVE BACTERIUM; GRAM STAINING; INTERMETHOD COMPARISON; NONHUMAN; TURNAROUND TIME;

ANTI-BACTERIAL AGENTS; BACTEREMIA; BACTERIAL TYPING TECHNIQUES; DRUG RESISTANCE, BACTERIAL; GRAM-NEGATIVE BACTERIA; GRAM-NEGATIVE BACTERIAL INFECTIONS; HUMANS; MICROBIAL SENSITIVITY TESTS;

EID: 84867803311     PISSN: 00365548     EISSN: 16511980     Source Type: Journal    
DOI: 10.3109/00365548.2012.689848     Document Type: Article

References (21)

1. Bruins MJ, Bloembergen P, Ruijs GJ, Wolfhagen MJ. Identification and susceptibility testing of Enterobacteriaceae and Pseudomonas aeruginosa by direct inoculation from positive BACTEC blood culture bottles into Vitek 2. J Clin Microbiol 2004;42:7 - 11.
2. Chen JR, Lee SY, Yang BH, Lu JJ. Rapid identification and susceptibility testing using the VITEK 2 system using culture fluids from positive BacT/ALERT blood cultures. J Microbiol Immunol Infect 2008;41:259 - 64.
3. de Cueto M, Ceballos E, Martinez-Martinez L, Perea EJ, Pascual A. Use of positive blood cultures for direct identification and susceptibility testing with the Vitek 2 system. J Clin Microbiol 2004;42:3734 - 8.
4. Fontanals D, Salceda F, Hernandez J, Sanfeliu I, Torra M. Evaluation of wider system for direct identification and antimicrobial susceptibility testing of Gram-negative bacilli from positive blood culture bottles. Eur J Clin Microbiol Infect Dis 2002;21:693 - 5.
5. Funke G, Funke-Kissling P. Use of the BD PHOENIX Automated Microbiology System for direct identification and susceptibility testing of Gram-negative rods from positive blood cultures in a three-phase trial. J Clin Microbiol 2004; 42:1466 - 70.
6. Hansen DS, Jensen AG, Norskov-Lauritsen N, Skov R, Bruun B. Direct identification and susceptibility testing of enteric bacilli from positive blood cultures using VITEK (GNI + /GNS-GA). Clin Microbiol Infect 2002;8:38 - 44.
7. Kerremans JJ, Verboom P, Stijnen T, Hakkaart-van Roijen L, Goessens W, Verbrugh HA, et al. Rapid identification and antimicrobial susceptibility testing reduce antibiotic use and accelerate pathogen-directed antibiotic use. J Antimicrob Chemother 2008;61:428 - 35.
8. Ling TK, Liu ZK, Cheng AF. Evaluation of the VITEK 2 system for rapid direct identification and susceptibility testing of Gram-negative bacilli from positive blood cultures. J Clin Microbiol 2003;41:4705 - 7.
9. Lupetti A, Barnini S, Castagna B, Nibbering PH, Campa M. Rapid identification and antimicrobial susceptibility testing of Gram-positive cocci in blood cultures by direct inoculation into the BD Phoenix system. Clin Microbiol Infect 2010; 16:986 - 91.
10. Waites KB, Brookings ES, Moser SA, Zimmer BL. Direct susceptibility testing with positive BacT/Alert blood cultures by using MicroScan overnight and rapid panels. J Clin Microbiol 1998;36:2052 - 6.
11. Munoz-Davila MJ, Yague G, Albert M, Garcia-Lucas T. Comparative evaluation of Vitek 2 identification and susceptibility testing of Gram-negative rods directly and isolated from BacT/ALERT-positive blood culture bottles. Eur J Clin Microbiol Infect Dis 2012;31:663-9.
12. Bryan CS, Reynolds KL, Brenner ER. Analysis of 1,186 episodes of Gram-negative bacteremia in non-university hospitals: the effects of antimicrobial therapy. Rev Infect Dis 1983;5:629 - 38.
13. Ibrahim EH, Sherman G, Ward S, Fraser VJ, Kollef MH. The influence of inadequate antimicrobial treatment of bloodstream infections on patient outcomes in the ICU setting. Chest 2000;118:146 - 55.
14. Kollef MH, Sherman G, Ward S, Fraser VJ. Inadequate antimicrobial treatment of infections: a risk factor for hospital mortality among critically ill patients. Chest 1999;115: 462 - 74.
15. Kreger BE, Craven DE, McCabe WR. Gram-negative bacteremia. IV. Re-evaluation of clinical features and treatment in 612 patients. Am J Med 1980;68:344 - 55.
16. Leibovici L, Paul M, Poznanski O, Drucker M, Samra Z, Konigsberger H, et al. Monotherapy versus beta-lactam - aminoglycoside combination treatment for Gram-negative bacteremia: a prospective, observational study. Antimicrob Agents Chemother 1997;41:1127 - 33.
17. Leibovici L, Shraga I, Drucker M, Konigsberger H, Samra Z, Pitlik SD. The benefit of appropriate empirical antibiotic treatment in patients with bloodstream infection. J Intern Med 1998;244:379 - 86.
18. Kumar A, Roberts D, Wood KE, Light B, Parrillo JE, Sharma S, et al. Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 2006;34: 1589 - 96.
19. Statens Helsetilsyn. Bruk av antibiotika i sykehus. Oslo: Elanders Publishing; 2001.
20. Loonen A, Jansz A, Stalpers J, Wolffs P, van den Brule A. An evaluation of three processing methods and the effect of reduced culture times for faster direct identification of pathogens from BacT/ALERT blood cultures by MALDITOF MS. Eur J Clin Microbiol Infect Dis 2012:1 - 9. 11 Nov 2012 [Epub ahead of print] .
21. Barenfanger J, Graham DR, Kolluri L, Sangwan G, Lawhorn J, Drake CA, et al. Decreased mortality associated with prompt Gram staining of blood cultures. Am J Clin Pathol 2008;130:870 - 6.