Real-time protein unfolding: A method for determining the kinetics of native protein denaturation using a quantitative real-time thermocycler

BioTechniques

Volumn 53, Issue 4, 2012, Pages 231-238

  Biggar, Kyle K ^a   Dawson, Neal J ^a   Storey, Kenneth B ^a  

^a CARLETON UNIVERSITY   (Canada)

Author keywords

Fluorescence; Hexokinase; Lysozyme; Protein stability; QRT PCR; SYPRO orange; Urea

Indexed keywords

HEXOKINASE; LYSOZYME; UREA;

ARTICLE; CONSTANTS AND COEFFICIENTS; CONTROLLED STUDY; KINETICS; LABORATORY DEVICE; METHODOLOGY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN DETERMINATION; PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN UNFOLDING; QUANTITATIVE ANALYSIS; SCIENTIST; TEMPERATURE; THERMOCYCLER;

DIFFERENTIAL THERMAL ANALYSIS; KINETICS; PROTEIN DENATURATION; PROTEIN STABILITY;

EID: 84867771644     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/0000113922     Document Type: Article

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