A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

Analytical Biochemistry

Volumn 431, Issue 1, 2012, Pages 1-3

  Kao, Chien Han ^a   Cheng, Chiu Min ^b   Chuang, Kuo Hsiang ^c   Chuang, Chih Hung ^d   Tzou, Shey Cherng ^e   Cheng, Ta Chun ^a   Hsieh, Yuan Chin ^a   Liao, Kuang Wen ^e   Wang, Yun Ming ^e   Chang, Long Sen ^f   Roffler, Steve R ^g   Chen, Fang Ming ^h   Cheng, Tian Lu ^a,f  

^a KAOHSIUNG MEDICAL UNIVERSITY   (Taiwan)

^b NATIONAL KAOHSIUNG UNIVERSITY OF APPLIED SCIENCES   (Taiwan)

^c TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^d NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

^e NATIONAL CHIAO TUNG UNIVERSITY   (Taiwan)

^f NATIONAL SUN YAT SEN UNIVERSITY   (Taiwan)

^g INSTITUTE OF BIOMEDICAL SCIENCES   (Taiwan)

^h KAOHSIUNG MEDICAL UNIVERSITY   (Taiwan)

Author keywords

14 Epitope tag protein ladder; Anti epitope tag antibodies; Epitope tags; Protein molecular estimation; Sodium dodecyl sulfate polyacrylamide gel electrophoresis; Western blot analysis

Indexed keywords

ANTIBODIES; ELECTROPHORESIS; LADDERS; MOLECULAR WEIGHT; RECOMBINANT PROTEINS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

HIGHLY-CORRELATED; IMMUNOBLOTS; RELATIVE MOBILITY; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTS; WESTERN-BLOT ANALYSIS;

EPITOPES;

EPITOPE; MEGA TAG PROTEIN LADDER; PROTEIN TAG; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; GENE; MOLECULAR IMAGING; MOLECULAR WEIGHT; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; WESTERN BLOTTING;

EID: 84867698112     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.08.001     Document Type: Article

References (14)

1. H. Towbin, T. Staehelin, and J. Gordon Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications Proc. Natl. Acad. Sci. USA 76 1979 4350 4354
2. V.C. Tsang, K. Hancock, and A.R. Simons Calibration of prestained protein molecular weight standards for use in the "Western" or enzyme-linked immunoelectrotransfer blot techniques Anal. Biochem. 143 1984 304 307
3. D. Parkinson, and J.D. Redshaw Visible labeling of proteins for polyacrylamide gel electrophoresis with dabsyl chloride Anal. Biochem. 141 1984 121 126
4. M.M. Compton, S.A. Lapp, and R. Pedemonte Generation of multicolored, prestained molecular weight markers for gel electrophoresis Electrophoresis 23 2002 3262 3265
5. D. Della-Penna, R.E. Christoffersen, and A.B. Bennett Biotinylated proteins as molecular weight standards on Western blots Anal. Biochem. 152 1986 329 332
6. M. Neumaier, U. Fenger, and C. Wagener Transblot studies with biotin-labeled proteins: electrophoretic mobilities and detection limits Anal. Biochem. 156 1986 76 80
7. M. Chang, H.Y. Hsu, and H.J. Lee Dye-free protein molecular weight markers Electrophoresis 26 2005 3062 3068
8. C. Lindbladh, K. Mosbach, and L. Bulow Standard calibration proteins for Western blotting obtained by genetically prepared protein A conjugates Anal. Biochem. 197 1991 187 190
9. Y.M. Zhang, L. Geng, and D.W. Huang Generate Western blot protein marker from a single construct Anal. Biochem. 390 2009 206 208
10. R.R. Burgess, T.M. Arthur, and B.C. Pietz Mapping protein-protein interaction domains using ordered fragment ladder far-Western analysis of hexahistidine-tagged fusion proteins Methods Enzymol. 328 2000 141 157
11. K. Daskalow, P. Boisguerin, B. Jandrig, R. Volkmer, B. Micheel, and J.A. Schenk Epitope mapping of antibodies against S-tagged fusion proteins and molecular weight markers Biosci. Biotechnol. Biochem. 72 2008 346 351
12. K. Terpe Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems Appl. Microbiol. Biotechnol. 60 2003 523 533
13. R. Rydzanicz, X.S. Zhao, and P.E. Johnson Assembly PCR oligo maker: a tool for designing oligodeoxynucleotides for constructing long DNA molecules for RNA production Nucleic Acids Res. 33 2005 W521 W525
14. K. Nakajima, and Y. Yaoita Construction of multiple-epitope tag sequence by PCR for sensitive Western blot analysis Nucleic Acids Res. 25 1997 2231 2232