Quantification of Helicobacter pylori in gastric mucosa by real-time polymerase chain reaction: Comparison with traditional diagnostic methods

Diagnostic Microbiology and Infectious Disease

Volumn 74, Issue 3, 2012, Pages 248-252

  Belda, Sofía ^a   Saez, Jesús ^a   Santibáñez, Miguel ^b   Rodríguez, Juan Carlos ^a   Galiana, Antonio ^c   Sola Vera, Javier ^a   Ruiz García, Montserrat ^a   Brotons, Alicia ^a   López Girona, Elena ^a   Girona, Eva ^a   Sillero, Carlos ^a   Royo, Gloria ^a,c  

^a HOSPITAL GENERAL UNIVERSITARIO DE ELCHE   (Spain)

^b HOSPITAL UNIVERSITARIO MARQUÉS DE VALDECILLA   (Spain)

^c MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

Author keywords

Gastric mucosa; Helicobacter pylori; Real time PCR

Indexed keywords

AREA UNDER THE CURVE; ARTICLE; BACTERIUM DETECTION; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; HELICOBACTER INFECTION; HELICOBACTER PYLORI; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PREDICTIVE VALUE; PRIORITY JOURNAL; PROSPECTIVE STUDY; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STOMACH ANTRUM; STOMACH BIOPSY; STOMACH BODY; STOMACH MUCOSA; VALIDITY; YOUDEN INDEX;

BACTERIAL LOAD; BIOPSY; FEMALE; GASTRIC MUCOSA; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; MALE; PREDICTIVE VALUE OF TESTS; PROSPECTIVE STUDIES; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 84867649812     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2012.07.007     Document Type: Article

References (25)

1. Akada J.K., Ogura K., Dailidiene D., Dailide G., Cheverud J.M., Berg D.E. Helicobacter pylori tissue tropism: mouse-colonizing strains can target different gastric niches. Microbiology 2003, 149(7):1901-1909.
2. Belda S., Saez J., Santibáñez M., Rodríguez J.C., Sola-Vera J., Ruiz-García M., et al. Relationship between bacterial load, morbidity and cagA gene in patients infected by Helicobacter pylori. Clin Microbiol Infect 2012, 18:E251-E253.
3. Blaser M.J. Who are we? Indigenous microbes and the ecology of human diseases. EMBO Rep 2006, 7:956-960.
4. Bruden D.L., Bruce M.G., Miernyk K.M., Morris J., Hurlburt D., Hennessy T.W., et al. Diagnostic accuracy of tests for Helicobacter pylori in an Alaska Native population. W J Gastroenterol 2011, 17:4682-4688.
5. Calvet X., Sánchez-Delgado J., Montserrat A., Lario S., Ramírez-Lázaro M.J., Quesada M., et al. Accuracy of diagnostic tests for Helicobacter pylori: a reappraisal. Clin Infect Dis 2009, 48:1385-1391.
6. Flores E., et al. Real time PCR with internal amplification control form detecting tuberculosis: method design and validation. Acta Pathol Microbiol Inmunol Scand (APMIS) 2009, 117:392-397.
7. Fox J.G., Megraud F. Helicobacter. Manual of clinical microbiology 2007, 947-962. ASM Press, Washington, D.C. P.R. Murray, E.J. Baron, J.H. Jorgensen, M.L. Landry, M.A. Pfaller (Eds.).
8. Graham D.Y., Opekun A.R., Hammoud F., Yamaoka Y., Reddy R., Osato M.S., et al. Studies regarding the mechanism of false negative urea breath tests with proton pump inhibitors. Am J Gastroenterol 2003, 98:1005-1009.
9. Granstrom M., Lehours P., Bengtsson C., Mégraud F. Diagnosis of Helicobacter pylori. Helicobacter 2008, 13(Suppl 1):7-12.
10. Griñó P., Pascual S., Such J., Casellas J.A., Niveiro M., Andreu M., et al. Comparison of stool immunoassay with standard methods for detection of Helicobacter pylori infection in patients with upper-gastrointestinal bleeding of peptic origin. Eur J Gastroenterol Hepatol 2003, 15:525-529.
11. Hirschl A.M., Makristathis A. Methods to detect Helicobacter pylori: from culture to molecular biology. Helicobacter 2007, 12(Suppl 2):6-11.
12. Islam S., Weilert F., Babington R., Dickson G., Smith A.C. Stool antigen testing for the diagnosis and confirmation of eradication of Helicobacter pylori infection: a prospective blinded trial. Intern Med J 2005, 35:526-529.
13. Lai Y.C., Wang T.H., Huang S.H., Yang S.S., Wu C.H., Chen T.K., et al. Density of Helicobacter pylori may affect the efficacy of eradication therapy and ulcer healing in patients with active duodenal ulcers. World J Gastroenterol 2003, 9:1537-1540.
14. Lascols C., Lamarque D., Costa J.M., Copie-Bergman C., Le Glaunec J.M., Deforges L., et al. Fast and accurate quantitative detection of Helicobacter pylori and identification of clarithromycin resistance mutations in H. pylori isolates from gastric biopsy specimens by real-time PCR. J Clin Microbiol 2003, 41:4573-4577.
15. Macgraud F., Lehours P. Helicobacter pylori detection and antimicrobial susceptibility testing. Clin Microbiol Rev 2007, 20:280-322.
16. Mishra S., Singh V., Rao G.R., Dixit V.K., Gulati A.K., Nath G. Prevalence of Helicobacter pylori in asymptomatic subjects-a nested PCR based study. Infect Genet Evol 2008, 8:815-819.
17. Peleteiro B., Lunet N., Barros R., La Vecchia C., Barros H. Factors contributing to the underestimation of Helicobacter pylori-associated gastric cancer risk in a high-prevalence population. Cancer Causes Control 2010, 21:1257-1264.
18. Roussel Y., Harris A., Lee M.H., Wilks M. Novel methods of quantitative real-time PCR data analysis in a murine Helicobacter pylori vaccine model. Vaccine 2007, 25:2919-2929.
19. Ruzsovics A., Molnar B., Tulassay Z. Deoxyribonucleic acid-based diagnostic techniques to detect Helicobacter pylori. Aliment Pharmacol Ther 2004, 19:1137-1146.
20. Schabereiter-Gurtner C., Hirschl A.M., Dragosics B., Hufnagl P., Puz S., Kovách Z., et al. Novel real-time PCR assay for detection of Helicobacter pylori infection and simultaneous clarithromycin susceptibility testing of stool and biopsy specimens. J Clin Microbiol 2004, 42:4512-4518.
21. Schilling D., Demel A., Adamek H.E., Nusse T., Weidmann E., Riemann J.F. A negative rapid urease test is unreliable for exclusion of Helicobacter pylori infection during acute phase of ulcer bleeding. A prospective case control study. Dig Liver Dis 2003, 35:217-221.
22. Simala-Grant J.L., Taylor D.E. Molecular biology methods for the characterization of Helicobacter pylori infections and their diagnosis. APMIS 2004, 112:886-897.
23. Van IJzendoorn M.C., Laheij R.J., de Boer W.A., Jansen J.B. The importance of corpus biopsies for the determination of Helicobacter pylori infection. Neth J Med 2005, 63:141-145.
24. Yakoob J., Jafri W., Abbas Z., Abid S., Islam M., Ahmed Z. The diagnostic yield of various tests for helicobacter pylori infection in patients on acid-reducing drugs. Dig Dis Sci 2008, 53:95-100.
25. Zweig M.H., Campbell G. Receiver-operating characteristic (ROC) plots: a fundamental evaluation tool in clinical medicine. Clin Chem 1993, 39:561-577.