Epithelial mucosa as an alternative tissue for DNA extraction in amphibians

Conservation Genetics Resources

Volumn 4, Issue 4, 2012, Pages 1097-1099

  Mendoza, Ángela María ^a   García Ramirez, Juan Carlos ^b   Cárdenas Henao, Heiber ^c  

^a INSTITUTO DE INVESTIGACIONES EN ECOSISTEMAS Y SUSTENTABILIDAD   (Mexico)

^b MASSEY UNIVERSITY   (New Zealand)

^c UNIVERSIDAD DEL VALLE   (Colombia)

Author keywords

Amphibians; Comparison protocols; DNA extraction; Epithelial Mucosa; mtDNA; Non destructive sampling

Indexed keywords

EID: 84867605081     PISSN: 18777252     EISSN: 18777260     Source Type: Journal    
DOI: 10.1007/s12686-012-9714-6     Document Type: Article

References (19)

1. Barbour T (1914) A contribution to the zoogeography of the West Indies, with special reference to amphibians and reptiles. Memoirs of the museum of comparative zoology. Cambridge, Massachusetts Vol 44, p 205-359.
2. Bessetti J (2007) An introduction to PCR inhibitors. Profiles DNA 10: 9-10.
3. Egloff C, Lambrosse A, Hebert C, Crump D (2009) A nondestructive method for obtaining maternal DNA from avian eggshells and its application to embryonic viability determination in herring gulls (Larus argentatus). Mol Ecol Resour 9: 19-27.
4. Funk WC, Donnelly MA, Lips KR (2005) Alternative views of amphibian toe-clipping. Nature 433: 193.
5. Goldberg CS, Kaplan ME, Schwalbe CR (2003) From the frog's mouth: buccal swabs for collection of DNA from amphibians. Herptol Rev 34: 220-221.
6. Gosner RA, Collura RV (1996) Waste not, want not: toe-clips as a source of DNA. J Herpetol 30: 445-447.
7. Hleap JS, Cárdenas H, García-Vallejo F (2009) Preservación no criogénica de tejido y extracción de DNA: una aplicación para peces cartilaginosos. PanamJAS 4: 545-555.
8. Kaiser H, Barrio-Amoros CL, Trujillo JD, Lynch JD (2002) Expansion of Eleutherodactylus johnstonei in northern South America. Herptol Rev 33: 290-294.
9. Kessing B, Croom H, Martin A, McIntosh C, Owen MW, and Palumbi SP (2004) The simple fool's guide to PCR, version 1. 0. Special publication. Dept. of Zoology, University of Hawaii, Honolulu, USA.
10. Lopera-Barrero NM, Ribeiro RP, Sirol RN, Povh JA, Gomes PC, Streit DP Jr, Vargas LM (2008) Comparison of DNA extraction protocols of fish fin and larvae samples: modified salt (NaCl) extraction. Cienc Investig Agrar 35: 65-74.
11. Lucentini L, Palomba A, Lacioni H, Natali M, Fausto P (2006) A nondestructive, rapid, reliable and inexpensive method to sample, store and extract high-quality DNA from fish body mucus and buccal cells. Mol Ecol Notes 6: 257-260.
12. Mendoza AM, García-Ramírez JC, Cardenas-Henao H (2012) Blood puncture as a non-destructive sampling tool to obtain DNA in frogs: comparison of protocols and survival analysis. Mol Ecol Res 12: 470-475.
13. Parris KM, McCall SC, McCarthy MA, Minteer BA, Steele K, Bekessy S, Medvecky F (2010) Assessing ethical trade-offs in ecological field studies. J Appl Ecol 47: 227-234.
14. Perry G, Wallace MC, Perry D, Curzer H, Muhlberger P (2011) Toe clipping of amphibians and reptiles: science, ethics, and the law. J Herpetol 45: 547-555.
15. Pidancier N, Miquel NC, Miaud C (2003) Buccal swabs as a nondestructive tissue sampling method for DNA analysis in amphibians. Herpetol J 13: 175-178.
16. Poschadel JR, Moller D (2004) A versatile field method for tissue sampling on small reptiles and amphibians, applied to ponds turtles, newts, frogs and toads. Conserv Genet 5: 865-867.
17. Prunier J, Kaufmann B, Grolet O, Picard D, Pompanon F, Joly P (2012) Skin swabbing as a new efficient DNA sampling technique in amphibians, and 14 new microsatellite markers in the alpine newt (Ichthyosaura alpestris). Mol Ecol Res 12: 524-553. doi: 10. 1111/j. 1755-0998. 2012. 03116. x.
18. Taberlet R, Walts LP, Luckart G (1999) Noninvasive genetic sampling: look before you leap. Trends Ecol Evol 14: 323-327.
19. Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-513.