Rapid molecular detection of extended-spectrum β-lactamase gene variants with a novel ligation-mediated real-time PCR

Journal of Medical Microbiology

Volumn 61, Issue PART 11, 2012, Pages 1563-1567

  Nijhuis, Roel ^a   van Zwet, Anton ^a   Stuart, James Cohen ^b   Weijers, Thijs ^a   Savelkoul, Paul ^c  

^a Department of Medical Microbiology and Medical Immunology ^*  (Netherlands)

^b UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^c VU UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CARBAPENEMASE; EXTENDED SPECTRUM BETA LACTAMASE;

ARTICLE; BACTERIUM ISOLATE; COMBINATION DISC TEST; ENTEROBACTERIACEAE; GENETIC VARIABILITY; LIGATION MEDIATED REAL TIME POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEIC ACID ANALYSIS; PHENOTYPE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANTI-BACTERIAL AGENTS; BETA-LACTAMASES; DNA, BACTERIAL; DRUG RESISTANCE, MULTIPLE, BACTERIAL; ENTEROBACTERIACEAE; GENETIC VARIATION; PHENOTYPE; PREDICTIVE VALUE OF TESTS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 84867541979     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.047910-0     Document Type: Article

References (26)

1. Beceiro, A., Maharjan, S., Gaulton, T., Doumith, M., Soares, N. C., Dhanji, H., Warner, M., Doyle, M., Hickey, M. & other authors (2011). False extended-spectrum b-lactamase phenotype in clinical isolates of Escherichia coli associated with increased expression of OXA-1 or TEM-1 penicillinases and loss of porins. J Antimicrob Chemother 66, 2006-2010.
2. Bonnet, R. (2004). Growing group of extended-spectrum blactamases: the CTX-M enzymes. Antimicrob Agents Chemother 48, 1-14.
3. Bradford, P. A. (2001). Extended-spectrum b-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev 14, 933-951.
4. Cantón, R., Novais, A., Valverde, A., Machado, E., Peixe, L., Baquero, F. & Coque, T. M. (2008). Prevalence and spread of extendedspectrum b-lactamase-producing Enterobacteriaceae in Europe. Clin Microbiol Infect 14 (Suppl. 1), 144-153.
5. Carter, M. W., Oakton, K. J., Warner, M. & Livermore, D. M. (2000). Detection of extended-spectrum β-lactamases in klebsiellae with the Oxoid combination disk method. J Clin Microbiol 38, 4228-4232.
6. CLSI (2009). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard, 8th edn. Wayne, PA: Clinical Laboratory Standards Institute.
7. Cohen Stuart, J., Dierikx, C., Al Naiemi, N., Karczmarek, A., Van Hoek, A. H. A. M., Vos, P., Fluit, A. C., Scharringa, J., Duim, B. & other authors (2010). Rapid detection of TEM, SHV and CTX-M extendedspectrum β-lactamases in Enterobacteriaceae using ligation-mediated amplification with microarray analysis. J Antimicrob Chemother 65, 1377-1381.
8. Cuzon, G., Naas, T., Bogaerts, P., Glupczynski, Y. & Nordmann, P. (2012). Evaluation of a DNA microarray for the rapid detection of extended-spectrum β-lactamases (TEM, SHV and CTX-M), plasmidmediated cephalosporinases (CMY-2-like, DHA, FOX, ACC-1, ACT/ MIR and CMY-1-like/MOX) and carbapenemases (KPC, OXA-48, VIM, IMP and NDM). J Antimicrob Chemother 67, 1865-1869.
9. Dallenne, C., Da Costa, A., Decré, D., Favier, C. & Arlet, G. (2010). Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae. J Antimicrob Chemother 65, 490-495.
10. Drieux, L., Brossier, F., Sougakoff, W. & Jarlier, V. (2008). Phenotypic detection of extended-spectrum b-lactamase production in Enterobacteriaceae: review and bench guide. Clin Microbiol Infect 14 (Suppl. 1), 90-103.
11. Endimiani, A., Hujer, A. M., Hujer, K. M., Gatta, J. A., Schriver, A. C., Jacobs, M. R., Rice, L. B. & Bonomo, R. A. (2010). Evaluation of a commercial microarray system for detection of SHV-, TEM-, CTXM-, and KPC-type β-lactamase genes in Gram-negative isolates. J Clin Microbiol 48, 2618-2622.
12. Garrec, H., Drieux-Rouzet, L., Golmard, J.-L., Jarlier, V. & Robert, J. (2011). Comparison of nine phenotypic methods for detection of extended-spectrum β-lactamase production by Enterobacteriaceae. J Clin Microbiol 49, 1048-1057.
13. Gniadkowski, M. (2008). Evolution of extended-spectrum β-lactamases by mutation. Clin Microbiol Infect 14 (Suppl. 1), 11-32.
14. Kluytmans-Vandenbergh, M. F. Q., Kluytmans, J. A. J. W. & Voss, A. (2005). Dutch guideline for preventing nosocomial transmission of highly resistant microorganisms (HRMO). Infection 33, 309-313.
15. Kohner, P. C., Robberts, F. J. L., Cockerill, F. R., III & Patel, R. (2009). Cephalosporin MIC distribution of extended-spectrum-β-lactamaseand pAmpC-producing Escherichia coli and Klebsiella species. J Clin Microbiol 47, 2419-2425.
16. Nijhuis, R. H. T., van Zwet, A. A., Savelkoul, P. H. M., Roovers, E. A., Bosboom, R. W., Postma, B. & van Griethuysen, A. J. (2011). Distribution of extended-spectrum β-lactamase genes using a commercial DNA micro-array system. J Hosp Infect 79, 349-353.
17. Nijssen, S., Florijn, A., Bonten, M. J. M., Schmitz, F. J., Verhoef, J. & Fluit, A. C. (2004). β-Lactam susceptibilities and prevalence of ESBL-producing isolates among more than 5000 European Enterobacteriaceae isolates. Int J Antimicrob Agents 24, 585-591.
18. Queenan, A. M., Foleno, B., Gownley, C., Wira, E. & Bush, K. (2004). Effects of inoculum and β-lactamase activity in AmpC-and extendedspectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae clinical isolates tested by using NCCLS ESBL methodology. J Clin Microbiol 42, 269-275.
19. Quirante, O. F., Cerrato, S. G. & Pardos, S. L. (2011). Risk factors for bloodstream infections caused by extended-spectrum b-lactamaseproducing Escherichia coli and Klebsiella pneumoniae. Braz J Infect Dis 15, 370-376.
20. Robberts, F. J. L., Kohner, P. C. & Patel, R. (2009). Unreliable extended-spectrum β-lactamase detection in the presence of plasmidmediated AmpC in Escherichia coli clinical isolates. J Clin Microbiol 47, 358-361.
21. Song, K.-H., Jeon, J. H., Park, W. B., Park, S.-W., Kim, H. B., Oh, M. D., Lee, H.-S., Kim, N. J. & Choe, K. W. (2009). Clinical outcomes of spontaneous bacterial peritonitis due to extended-spectrum blactamase-producing Escherichia coli and Klebsiella species: a retrospective matched case-control study. BMC Infect Dis 9, 41.
22. van Doornum, G. J. J., Guldemeester, J., Osterhaus, A. D. M. E. & Niesters, H. G. M. (2003). Diagnosing herpesvirus infections by realtime amplification and rapid culture. J Clin Microbiol 41, 576-580.
23. Voets, G. M., Fluit, A. C., Scharringa, J., Cohen Stuart, J. & Leverstein-van Hall, M. A. (2011). A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases. Int J Antimicrob Agents 37, 356-359.
24. Wassenberg, M. W. M., Kluytmans, J. A., Box, A. T. A., Bosboom, R. W., Buiting, A. G. M., van Elzakker, E. P. M., Melchers, W. J. G., van Rijen, M. M. L., Thijsen, S. F. T. & other authors (2010). Rapid screening of methicillin-resistant Staphylococcus aureus using PCR and chromogenic agar: a prospective study to evaluate costs and effects. Clin Microbiol Infect 16, 1754-1761.
25. Willemsen, I., Overdevest, I., Al Naiemi, N., Rijnsburger, M., Savelkoul, P., Vandenbroucke-Grauls, C., Kluytmans, J. & TRIANGLe Study Group (2011). New diagnostic microarray (Check-KPC ESBL) for detection and identification of extended-spectrum β-lactamases in highly resistant Enterobacteriaceae. J Clin Microbiol 49, 2985-2987.
26. Wu, T.-L., Siu, L. K., Su, L.-H., Lauderdale, T. L., Lin, F. M., Leu, H.-S., Lin, T.-Y. & Ho, M. (2001). Outer membrane protein change combined with co-existing TEM-1 and SHV-1 β-lactamases lead to false identification of ESBL-producing Klebsiella pneumoniae. J Antimicrob Chemother 47, 755-761.