Heterogeneity in White Blood Cells Has Potential to Confound DNA Methylation Measurements

PLoS ONE

Volumn 7, Issue 10, 2012, Pages

  Adalsteinsson, Bjorn T ^a,b   Gudnason, Haukur ^a   Aspelund, Thor ^a,b   Harris, Tamara B ^c   Launer, Lenore J ^c   Eiriksdottir, Gudny ^a   Smith, Albert V ^a,b   Gudnason, Vilmundur ^a,b  

^a ICELANDIC HEART ASSOCIATION   (Iceland)

^b UNIVERSITY OF ICELAND   (Iceland)

^c NATIONAL INSTITUTE ON AGING   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; INWARDLY RECTIFYING POTASSIUM CHANNEL SUBUNIT KIR6.2;

ARTICLE; BASOPHIL; CELL FRACTIONATION; CELL HETEROGENEITY; CELL ISOLATION; CELL TYPE; CONTROLLED STUDY; CPG ISLAND; DENSITY GRADIENT CENTRIFUGATION; DNA DETERMINATION; DNA METHYLATION; EOSINOPHIL COUNT; FEMALE; GENE; GENE LOCATION; GENETIC DIFFERENCE; GENETIC VARIABILITY; HHEX GENE; HUMAN; HUMAN CELL; KCNJ11 GENE; KCNQ1 GENE; LEUKOCYTE COUNT; LYMPHOCYTE COUNT; MALE; MONOCYTE; MONONUCLEAR CELL; NEUTROPHIL COUNT; PM20D1 ENE; POLYMORPHONUCLEAR CELL; PYROSEQUENCING;

ADULT; AGED; AGED, 80 AND OVER; CPG ISLANDS; DNA METHYLATION; EPIGENESIS, GENETIC; FEMALE; GENETIC VARIATION; HOMEODOMAIN PROTEINS; HUMANS; KCNQ1 POTASSIUM CHANNEL; LEUKOCYTES, MONONUCLEAR; MALE; MIDDLE AGED; NEUTROPHILS; POTASSIUM CHANNELS, INWARDLY RECTIFYING; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION FACTORS;

EID: 84867185999     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0046705     Document Type: Article

References (23)

1. Eckhardt F, Lewin J, Cortese R, Rakyan VK, Attwood J, et al. (2006) DNA methylation profiling of human chromosomes 6, 20 and 22. Nat Genet 38: 1378-1385.
2. Fan S, Zhang X, (2009) CpG island methylation pattern in different human tissues and its correlation with gene expression. Biochem Biophys Res Commun 383: 421-425.
3. Irizarry RA, Ladd-Acosta C, Wen B, Wu Z, Montano C, et al. (2009) The human colon cancer methylome shows similar hypo- and hypermethylation at conserved tissue-specific CpG island shores. Nat Genet 41: 178-186.
4. Illingworth R, Kerr A, DeSousa D, Jørgensen H, Ellis P, et al. (2008) A Novel CpG Island Set Identifies Tissue-Specific Methylation at Developmental Gene Loci. PLoS Biol 6: e22.
5. De Bustos C, Ramos E, Young JM, Tran RK, Menzel U, et al. (2009) Tissue-specific variation in DNA methylation levels along human chromosome I. Epigenetics Chromatin 2: 7.
6. Kochanek S, Toth M, Dehmel A, Renz D, Doerfler W, (1990) Interindividual concordance of methylation profiles in human genes for tumor necrosis factors α and β. Proc Natl Acad Sci USA 87: 8830-8834.
7. Kochanek S, Radbruch A, Tesch H, Renz D, Doerfler W, (1991) DNA methylation profiles in the human genes for tumor necrosis factors α and β in subpopulations of leukocytes and in leukemias. Proc Natl Acad Sci USA 88: 5759-5763.
8. Wu HC, Delgado-Cruzata L, Flom JD, Kappil M, Ferris JS, et al. (2011) Global methylation profiles in DNA from different blood cell types. Epigenetics 6: 76-85.
9. Martin GM, (2005) Epigenetic drift in aging identical twins. Proc Natl Acad Sci USA 102: 10413-10414.
10. Talens RP, Boomsma DI, Tobi EW, Kremer D, Jukema JW, et al. (2010) Variation, patterns, and temporal stability of DNA methylation: considerations for epigenetic epidemiology. Faseb J 24: 3135-3144.
11. Feinberg AP, Irizarry RA, Fradin D, Aryee MJ, Murakami P, et al. (2010) Personalized Epigenomic Signatures That Are Stable Over Time and Covary with Body Mass Index. Sci Transl Med 2: 49ra67.
12. Kent WJ, Sugnet CW, Furey TS, Roskin KM, Pringle TH, et al. (2002) The Human Genome Browser at UCSC. Genome Res 12: 996-1006.
13. Crompton MR, Bartlett TJ, MacGregor AD, Manfioletti G, Buratti E, et al. (1992) Identification of a novel vertebrate homeobox gene expressed in haematopoietic cells. Nucleic Acids Res 20: 5661-5667.
14. Bedford FK, Ashworth A, Enver T, Wiedemann LM, (1993) HEX: a novel homeobox gene expressed during haematopoiesis and conserved between mouse and human. Nucleic Acids Res 21: 1245-1249.
15. Manfioletti G, Gattei V, Buratti E, Rustighi A, De Iuliis A, et al. (1995) Differential expression of a novel proline-rich homeobox gene (Prh) in human hematolymphopoietic cells. Blood 85: 1237-1245.
16. Kerkel K, Schupf N, Hatta K, Pang D, Salas M, et al. (2010) Altered DNA Methylation in Leukocytes with Trisomy 21. PLoS Genet 6: e1001212.
17. Koestler DC, Marsit CJ, Christensen BC, Accomando W, Langevin SM, et al. (2012) Peripheral blood immune cell methylation profiles are associated with non-hematopoietic cancers. Cancer Epidemiol Biomarkers Prev In press.
18. Houseman EA, Accomando WP, Koestler DC, Christensen BC, Marsit CJ, et al. (2012) DNA methylation arrays as surrogate measures of cell mixture distribution. BMC Bioinformatics 13: 86 In press.
19. Margolis KL, Rodabough RJ, Thomson CA, Lopez AM, McTiernan A, (2007) Prospective Study of Leukocyte Count as a Predictor of Incident Breast, Colorectal, Endometrial, and Lung Cancer and Mortality in Postmenopausal Women. Arch Intern Med 167: 1837-1844.
20. Danesh J, Collins R, Appleby P, Peto R, (1998) Association of fibrinogen, C-reactive protein, albumin, or leukocyte count with coronary heart disease: meta-analyses of prospective studies. JAMA 279: 1477-1482.
21. Byun HM, Siegmund KD, Pan F, Weisenberger DJ, Kanel G, et al. (2009) Epigenetic profiling of somatic tissues from human autopsy specimens identifies tissue- and individual-specific DNA methylation patterns. Hum Mol Genet 18: 4808-4817.
22. Bjornsson HT, Sigurdsson MI, Fallin MD, Irizarry RA, Aspelund T, et al. (2008) Intra-individual Change Over Time in DNA Methylation With Familial Clustering. Jama 299: 2877-2883.
23. Harris TB, Launer LJ, Eiriksdottir G, Kjartansson O, Jonsson PV, et al. (2007) Age, Gene/Environment Susceptibility-Reykjavik Study: multidisciplinary applied phenomics. Am J Epidemiol 165: 1076-1087.